1. Deoxyribonucleic acid replication in fetal cells
- Author
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Sagesaka, Toshiaki, Boubnov, Nikolai, Okuyama, Teruaki, Paulus, Henry, and Sarkar, Nilima
- Subjects
Fetal tissues -- Growth ,Cell culture -- Methods ,DNA ,Hydroxyurea -- Usage ,Health - Abstract
Growth factors in modified culture mediums, hydroxyurea and flow cytometry appear to be helpful in the evaluation of the DNA replication time of human genes in fetal cells. Culture mediums are substances used to support the growth of microorganisms or other cells. Hydroxyurea inhibits enzymes involved in DNA synthesis. Flow cytometry is a method of counting cells. Researchers cultured human male and female fetal lung cells in a variety of growth mediums and studied DNA replication. Flow cytometry indicated a high degree of synchronization among the fetal cells during the S phase of the cell cycle, which is the phase in which DNA is synthesized. DNA synthesis was completed in a well-defined time period of seven hours. In addition, the replication of the human topoisomerase II gene occurred in a short time span only three hours after entry into the S phase.
- Published
- 1994