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Your search keyword '"Potapenko, A. I."' showing total 3 results
Start Over Author "Potapenko, A. I." Topic dna
3 results on '"Potapenko, A. I."'

1. [A trial of the enzyme nuclease S1 for the recognition of defects in the secondary structure of DNA].

Author

Potapenko AI and Obukhova LL

Subjects
Aging drug effects, Aging genetics, Aging radiation effects, Animals, Calibration, DNA radiation effects, DNA, Single-Stranded drug effects, DNA, Single-Stranded radiation effects, Dose-Response Relationship, Radiation, Enzyme Stability, Mice, Nucleic Acid Conformation radiation effects, Plasmids, DNA drug effects, DNA Damage, Nucleic Acid Conformation drug effects, Single-Strand Specific DNA and RNA Endonucleases pharmacology
Abstract

In order to develop an adequate method for quantitative determination of age-related defects of DNA secondary structure in somatic cells, the testing of enzyme nuclease S1 ("Sigma") was carried out. The conditions have been selected under which this enzyme revealed all single-stranded breaks and did not induce nonspecific breaks in DNA. It was shown that homopurine-homopyridine blocks of DNA capable of changing to H-form hypersensitive to S1, were not detected with the enzyme in the selected conditions.

Published
1992

2. [Defects in the secondary structure of DNA recognizable by nuclease S1 and their possible role in the aging of mammalian somatic cells].

Author

Potapenko AI and Obukhova LK

Subjects
Aging drug effects, Aging radiation effects, Animals, Base Composition drug effects, Base Composition radiation effects, DNA radiation effects, Female, Liver drug effects, Liver radiation effects, Mice, Mice, Inbred CBA, Nucleic Acid Conformation radiation effects, Aging genetics, DNA drug effects, DNA Damage, Nucleic Acid Conformation drug effects, Single-Strand Specific DNA and RNA Endonucleases pharmacology
Abstract

The amount of defects in DNA secondary structure recognized by nuclease S1 and their localization by sequences of various nucleotide composition were studied in relation to the age factor. It was established that a significant twofold increase in defects of DNA secondary structure was observed in hepatic cells of very old (30 months and older) intact mice only, as well as in radiation-induced acceleration of aging at the age 19 months. The prevailing localization of the defects of DNA secondary structure has been demonstrated in those sequences which were enriched with AT pairs by 3% (as compared with the average level). It was concluded that the mentioned defects of DNA secondary structure are not the cause of aging. Nevertheless they can play an essential role in the process of irreversible destruction of genome in the terminal phase of aging.

Published
1992

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