1. DNA fluorescent labeling with naphtho[1,2,3-cd]indol-6(2H)-one for investigation of protein-DNA interactions.
- Author
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Vasilyeva SV, Kuznetsov NA, Kuznetsova AS, Khalyavina JG, Tropina DA, Lavrikova TI, Kargina OI, and Gornostaev LM
- Subjects
- DNA Glycosylases metabolism, Fluorescent Dyes chemical synthesis, Humans, Indoles chemical synthesis, Molecular Structure, Naphthalenes chemical synthesis, Structure-Activity Relationship, DNA chemistry, DNA Glycosylases chemistry, Fluorescence, Fluorescent Dyes chemistry, Indoles chemistry, Naphthalenes chemistry
- Abstract
Fluorescently labeled DNA to study protein-DNA interactions was synthesized using the Cu(I)-catalysed cycloaddition (CuAAC) reaction. For this purpose, a new azido-containing fluorophore based on the naphtho[1,2,3-cd]indol-6(2H)-one derivative was obtained. The fluorescent properties of naphtho[1,2,3-cd]indol-6(2H)-one derivatives and labeled DNA were studied. The new fluorescent DNA conjugate was shown to be a useful tool to study complex mechanisms of protein-DNA interactions., (Copyright © 2017 Elsevier Inc. All rights reserved.) more...
- Published
- 2017
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