1. LIM protein Ajuba promotes liver cell proliferation through its involvement in DNA replication and DNA damage control.
- Author
-
Dommann N, Gavini J, Sánchez-Taltavull D, Baier FA, Birrer F, Loforese G, Candinas D, and Stroka D
- Subjects
- Animals, Cell Proliferation genetics, Mice, Mice, Knockout, DNA Damage, DNA Replication, LIM Domain Proteins genetics, LIM Domain Proteins metabolism, Liver cytology
- Abstract
The LIM-domain protein Ajuba is associated with cell proliferation, a fundamental process of tissue regeneration and cancer. We report that in the liver, Ajuba expression is increased during regeneration and in tumour cells and tissues. Knockout of Ajuba using CRISPR/Cas9 is embryonic lethal in mice. shRNA targeting of Ajuba reduces cell proliferation, delays cell entry into S-phase, reduces cell survival and tumour growth in vivo and increases expression of the DNA damage marker γH2AX. Ajuba binding partners include proteins involved in DNA replication and damage, such as SKP2, MCM2, MCM7 and RPA70. Taken together, our data support that Ajuba promotes liver cell proliferation associated with development, regeneration and tumour growth and is involved in DNA replication and damage repair., (© 2022 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)
- Published
- 2022
- Full Text
- View/download PDF