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Start Over Author "Piazzetta, Maria H. O." Topic dna damage
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1. An ultrasoft X-ray multi-microbeam irradiation system for studies of DNA damage responses by fixed- and live-cell fluorescence microscopy.

Author

van Oven C, Krawczyk PM, Stap J, Melo AM, Piazzetta MH, Gobbi AL, van Veen HA, Verhoeven J, and Aten JA

Subjects
Cell Line, Tumor, Green Fluorescent Proteins, Humans, Immunohistochemistry methods, Microscopy, Fluorescence methods, DNA radiation effects, DNA Damage radiation effects, DNA Repair, X-Rays
Abstract

Localized induction of DNA damage is a valuable tool for studying cellular DNA damage responses. In recent decades, methods have been developed to generate DNA damage using radiation of various types, including photons and charged particles. Here we describe a simple ultrasoft X-ray multi-microbeam system for high dose-rate, localized induction of DNA strand breaks in cells at spatially and geometrically adjustable sites. Our system can be combined with fixed- and live-cell microscopy to study responses of cells to DNA damage.

Published
2009
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