37 results on '"Just P"'
Search Results
2. X chromosome dosage and the genetic impact across human tissues
- Author
-
Viuff, Mette, Skakkebæk, Anne, Johannsen, Emma B., Chang, Simon, Pedersen, Steen Bønlykke, Lauritsen, Katrine Meyer, Pedersen, Mette Glavind Bülow, Trolle, Christian, Just, Jesper, and Gravholt, Claus H.
- Published
- 2023
- Full Text
- View/download PDF
3. Short- and intermediate-term exposure to ambient fine particulate elements and leukocyte epigenome-wide DNA methylation in older men: the Normative Aging Study
- Author
-
Wang, Cuicui, Cardenas, Andres, Hutchinson, John N, Just, Allan, Heiss, Jonathan, Hou, Lifang, Zheng, Yinan, Coull, Brent A, Kosheleva, Anna, Koutrakis, Petros, Baccarelli, Andrea A, and Schwartz, Joel D
- Subjects
Genetics ,Human Genome ,Aging ,Good Health and Well Being ,Aged ,Air Pollutants ,DNA Methylation ,Epigenome ,Humans ,Leukocytes ,Male ,Particulate Matter ,PM2.5 ,PM2.5 elments ,DNA methylation ,Epigenome-wide association study ,Distributed-lag ,Pathway analyses ,PM(2.5) ,PM(2.5) elements ,Environmental Sciences - Abstract
BackgroundSeveral epigenome-wide association studies (EWAS) of ambient particulate matter with aerodynamic diameter ≤ 2.5 µm (PM2.5) have been reported. However, EWAS of PM2.5 elements (PEs), reflecting different emission sources, are very limited.ObjectivesWe performed EWAS of short- and intermediate-term exposure to PM2.5 and 13 PEs. We hypothesized that significant changes in DNAm may vary by PM2.5 mass and its elements.MethodsWe repeatedly collected blood samples in the Normative Aging Study and measured leukocyte DNA methylation (DNAm) with the Illumina HumanMethylation450K BeadChip. We collected daily PM2.5 and 13 PEs at a fixed central site. To estimate the associations between each PE and DNAm at individual cytosine-phosphate-guanine (CpG) sites, we incorporated a distributed-lag (0-27 d) term in the setting of median regression with subject-specific intercept and examined cumulative lag associations. We also accounted for selection bias due to loss to follow-up and mortality prior to enrollment. Significantly differentially methylated probes (DMPs) were identified using Bonferroni correction for multiple testing. We further conducted regional and pathway analyses to identify significantly differentially methylated regions (DMRs) and pathways.ResultsWe included 695 men with 1,266 visits between 1999 and 2013. The subjects had a mean age of 75 years. The significant DMPs, DMRs, and pathways varied by to PM2.5 total mass and PEs. For example, PM2.5 total mass was associated with 2,717 DMPs and 10,470 DMRs whereas Pb was associated with 3,173 DMPs and 637 DMRs. The identified pathways by PM2.5 mass were mostly involved in mood disorders, neuroplasticity, immunity, and inflammation, whereas the pathways associated with motor vehicles (BC, Cu, Pb, and Zn) were related with cardiovascular disease and cancer (e.g., "PPARs signaling").ConclusionsPM2.5 and PE were associated with methylation changes at multiple probes and along multiple pathways, in ways that varied by particle components.
- Published
- 2022
4. Blood DNA methylation biomarkers of cumulative lead exposure in adults.
- Author
-
Colicino, Elena, Just, Allan, Kioumourtzoglou, Marianthi-Anna, Vokonas, Pantel, Cardenas, Andres, Sparrow, David, Weisskopf, Marc, Nie, Linda H, Hu, Howard, Schwartz, Joel D, Wright, Robert O, and Baccarelli, Andrea A
- Subjects
DNA methylation ,Epidemiology ,Exposure Modeling ,Metals ,Methods ,Personal exposure ,Chemical Sciences ,Environmental Sciences ,Medical and Health Sciences - Abstract
BackgroundLead is a ubiquitous toxicant following three compartment kinetics with the longest half-life found in bones. Patella and tibia lead levels-validated measures of cumulative exposure-require specialized X-ray-fluorescence-spectroscopy available only in a few centers worldwide. We developed minimally invasive biomarkers reflecting individual cumulative lead exposure using blood DNA methylation profiles-obtainable via Illumina 450K or IlluminaEPIC bead-chip assays.MethodsWe developed and tested two methylation-based biomarkers from 348 Normative Aging Study (NAS) elderly men. We selected methylation sites with strong associations with bone lead levels via robust regressions analysis and constructed the biomarkers using elastic nets. Results were validated in a NAS subset, reporting specificity, and sensitivity.FindingsParticipants were 73 years old on average (standard deviation, SD = 6), with moderate lead levels of (mean ± SD patella: 27 ± 18 µg/g; tibia:21 ± 13 µg/g). Methylation-based biomarkers for lead in patella and tibia included 59 and 138 DNA methylation sites, respectively. Estimated lead levels were significantly correlated with actual measured values, (r = 0.62 patella, r = 0.59 tibia) and had low mean square error (MSE) (MSE = 0.68 patella, MSE = 0.53 tibia). Means and distributions of the estimated and actual lead levels were not significantly different across patella and tibia bones (p > 0.05). Methylation-based biomarkers discriminated participants highly exposed (>median) to lead with a specificity of 74 and 73% for patella and tibia lead levels, respectively, with 70% sensitivity.InterpretationDNA methylation-based lead biomarkers are novel tools that can be used to reconstruct decades' worth of individual cumulative lead exposure using only blood DNA methylation profiles and may help identify the consequences of cumulative exposure.
- Published
- 2021
5. Blood Leukocyte DNA Methylation Predicts Risk of Future Myocardial Infarction and Coronary Heart Disease
- Author
-
Agha, Golareh, Mendelson, Michael M, Ward-Caviness, Cavin K, Joehanes, Roby, Huan, TianXiao, Gondalia, Rahul, Salfati, Elias, Brody, Jennifer A, Fiorito, Giovanni, Bressler, Jan, Chen, Brian H, Ligthart, Symen, Guarrera, Simonetta, Colicino, Elena, Just, Allan C, Wahl, Simone, Gieger, Christian, Vandiver, Amy R, Tanaka, Toshiko, Hernandez, Dena G, Pilling, Luke C, Singleton, Andrew B, Sacerdote, Carlotta, Krogh, Vittorio, Panico, Salvatore, Tumino, Rosario, Li, Yun, Zhang, Guosheng, Stewart, James D, Floyd, James S, Wiggins, Kerri L, Rotter, Jerome I, Multhaup, Michael, Bakulski, Kelly, Horvath, Steven, Tsao, Philip S, Absher, Devin M, Vokonas, Pantel, Hirschhorn, Joel, Fallin, M Daniele, Liu, Chunyu, Bandinelli, Stefania, Boerwinkle, Eric, Dehghan, Abbas, Schwartz, Joel D, Psaty, Bruce M, Feinberg, Andrew P, Hou, Lifang, Ferrucci, Luigi, Sotoodehnia, Nona, Matullo, Giuseppe, Peters, Annette, Fornage, Myriam, Assimes, Themistocles L, Whitsel, Eric A, Levy, Daniel, and Baccarelli, Andrea A
- Subjects
Human Genome ,Heart Disease ,Clinical Research ,Prevention ,Heart Disease - Coronary Heart Disease ,Atherosclerosis ,Cardiovascular ,Genetics ,Adult ,Aged ,Cohort Studies ,Coronary Disease ,CpG Islands ,DNA Methylation ,Europe ,Female ,Genome-Wide Association Study ,Humans ,Incidence ,Leukocytes ,Male ,Middle Aged ,Myocardial Infarction ,Population Groups ,Prognosis ,Prospective Studies ,Risk ,United States ,coronary artery disease ,coronary heart disease ,epigenetics ,genomics ,gene expression regulation ,Cardiorespiratory Medicine and Haematology ,Clinical Sciences ,Public Health and Health Services ,Cardiovascular System & Hematology - Abstract
BackgroundDNA methylation is implicated in coronary heart disease (CHD), but current evidence is based on small, cross-sectional studies. We examined blood DNA methylation in relation to incident CHD across multiple prospective cohorts.MethodsNine population-based cohorts from the United States and Europe profiled epigenome-wide blood leukocyte DNA methylation using the Illumina Infinium 450k microarray, and prospectively ascertained CHD events including coronary insufficiency/unstable angina, recognized myocardial infarction, coronary revascularization, and coronary death. Cohorts conducted race-specific analyses adjusted for age, sex, smoking, education, body mass index, blood cell type proportions, and technical variables. We conducted fixed-effect meta-analyses across cohorts.ResultsAmong 11 461 individuals (mean age 64 years, 67% women, 35% African American) free of CHD at baseline, 1895 developed CHD during a mean follow-up of 11.2 years. Methylation levels at 52 CpG (cytosine-phosphate-guanine) sites were associated with incident CHD or myocardial infarction (false discovery rate
- Published
- 2019
6. Meta-analysis of epigenome-wide association studies in neonates reveals widespread differential DNA methylation associated with birthweight.
- Author
-
Küpers, Leanne K, Monnereau, Claire, Sharp, Gemma C, Yousefi, Paul, Salas, Lucas A, Ghantous, Akram, Page, Christian M, Reese, Sarah E, Wilcox, Allen J, Czamara, Darina, Starling, Anne P, Novoloaca, Alexei, Lent, Samantha, Roy, Ritu, Hoyo, Cathrine, Breton, Carrie V, Allard, Catherine, Just, Allan C, Bakulski, Kelly M, Holloway, John W, Everson, Todd M, Xu, Cheng-Jian, Huang, Rae-Chi, van der Plaat, Diana A, Wielscher, Matthias, Merid, Simon Kebede, Ullemar, Vilhelmina, Rezwan, Faisal I, Lahti, Jari, van Dongen, Jenny, Langie, Sabine AS, Richardson, Tom G, Magnus, Maria C, Nohr, Ellen A, Xu, Zongli, Duijts, Liesbeth, Zhao, Shanshan, Zhang, Weiming, Plusquin, Michelle, DeMeo, Dawn L, Solomon, Olivia, Heimovaara, Joosje H, Jima, Dereje D, Gao, Lu, Bustamante, Mariona, Perron, Patrice, Wright, Robert O, Hertz-Picciotto, Irva, Zhang, Hongmei, Karagas, Margaret R, Gehring, Ulrike, Marsit, Carmen J, Beilin, Lawrence J, Vonk, Judith M, Jarvelin, Marjo-Riitta, Bergström, Anna, Örtqvist, Anne K, Ewart, Susan, Villa, Pia M, Moore, Sophie E, Willemsen, Gonneke, Standaert, Arnout RL, Håberg, Siri E, Sørensen, Thorkild IA, Taylor, Jack A, Räikkönen, Katri, Yang, Ivana V, Kechris, Katerina, Nawrot, Tim S, Silver, Matt J, Gong, Yun Yun, Richiardi, Lorenzo, Kogevinas, Manolis, Litonjua, Augusto A, Eskenazi, Brenda, Huen, Karen, Mbarek, Hamdi, Maguire, Rachel L, Dwyer, Terence, Vrijheid, Martine, Bouchard, Luigi, Baccarelli, Andrea A, Croen, Lisa A, Karmaus, Wilfried, Anderson, Denise, de Vries, Maaike, Sebert, Sylvain, Kere, Juha, Karlsson, Robert, Arshad, Syed Hasan, Hämäläinen, Esa, Routledge, Michael N, Boomsma, Dorret I, Feinberg, Andrew P, Newschaffer, Craig J, Govarts, Eva, Moisse, Matthieu, Fallin, M Daniele, Melén, Erik, and Prentice, Andrew M
- Subjects
Fetus ,Humans ,Prenatal Exposure Delayed Effects ,Birth Weight ,Folic Acid ,DNA ,Body Mass Index ,Smoking ,DNA Methylation ,Epigenesis ,Genetic ,CpG Islands ,Fetal Development ,Pregnancy ,Genome ,Human ,Adolescent ,Adult ,Child ,Infant ,Newborn ,Female ,Male ,Genome-Wide Association Study ,Epigenesis ,Genetic ,Genome ,Human ,Infant ,Newborn - Abstract
Birthweight is associated with health outcomes across the life course, DNA methylation may be an underlying mechanism. In this meta-analysis of epigenome-wide association studies of 8,825 neonates from 24 birth cohorts in the Pregnancy And Childhood Epigenetics Consortium, we find that DNA methylation in neonatal blood is associated with birthweight at 914 sites, with a difference in birthweight ranging from -183 to 178 grams per 10% increase in methylation (PBonferroni
- Published
- 2019
7. Cohort Profile: Pregnancy And Childhood Epigenetics (PACE) Consortium
- Author
-
Felix, Janine F, Joubert, Bonnie R, Baccarelli, Andrea A, Sharp, Gemma C, Almqvist, Catarina, Annesi-Maesano, Isabella, Arshad, Hasan, Baïz, Nour, Bakermans-Kranenburg, Marian J, Bakulski, Kelly M, Binder, Elisabeth B, Bouchard, Luigi, Breton, Carrie V, Brunekreef, Bert, Brunst, Kelly J, Burchard, Esteban G, Bustamante, Mariona, Chatzi, Leda, Munthe-Kaas, Monica Cheng, Corpeleijn, Eva, Czamara, Darina, Dabelea, Dana, Smith, George Davey, De Boever, Patrick, Duijts, Liesbeth, Dwyer, Terence, Eng, Celeste, Eskenazi, Brenda, Everson, Todd M, Falahi, Fahimeh, Fallin, M Daniele, Farchi, Sara, Fernandez, Mariana F, Gao, Lu, Gaunt, Tom R, Ghantous, Akram, Gillman, Matthew W, Gonseth, Semira, Grote, Veit, Gruzieva, Olena, Håberg, Siri E, Herceg, Zdenko, Hivert, Marie-France, Holland, Nina, Holloway, John W, Hoyo, Cathrine, Hu, Donglei, Huang, Rae-Chi, Huen, Karen, Järvelin, Marjo-Riitta, Jima, Dereje D, Just, Allan C, Karagas, Margaret R, Karlsson, Robert, Karmaus, Wilfried, Kechris, Katerina J, Kere, Juha, Kogevinas, Manolis, Koletzko, Berthold, Koppelman, Gerard H, Küpers, Leanne K, Ladd-Acosta, Christine, Lahti, Jari, Lambrechts, Nathalie, Langie, Sabine AS, Lie, Rolv T, Liu, Andrew H, Magnus, Maria C, Magnus, Per, Maguire, Rachel L, Marsit, Carmen J, McArdle, Wendy, Melén, Erik, Melton, Phillip, Murphy, Susan K, Nawrot, Tim S, Nisticò, Lorenza, Nohr, Ellen A, Nordlund, Björn, Nystad, Wenche, Oh, Sam S, Oken, Emily, Page, Christian M, Perron, Patrice, Pershagen, Göran, Pizzi, Costanza, Plusquin, Michelle, Raikkonen, Katri, Reese, Sarah E, Reischl, Eva, Richiardi, Lorenzo, Ring, Susan, Roy, Ritu P, Rzehak, Peter, Schoeters, Greet, Schwartz, David A, Sebert, Sylvain, Snieder, Harold, Sørensen, Thorkild IA, and Starling, Anne P
- Subjects
Epidemiology ,Public Health ,Health Sciences ,Statistics ,Mathematical Sciences ,Child Health ,Cohort Studies ,DNA Methylation ,Environmental Pollution ,Epigenesis ,Genetic ,Female ,Folic Acid ,Humans ,Infant ,Newborn ,Maternal Exposure ,Maternal Health ,Pregnancy ,Prenatal Exposure Delayed Effects ,Public Health and Health Services ,Public health - Published
- 2018
8. DNA Methylation Analysis Identifies Loci for Blood Pressure Regulation
- Author
-
Richard, Melissa A, Huan, Tianxiao, Ligthart, Symen, Gondalia, Rahul, Jhun, Min A, Brody, Jennifer A, Irvin, Marguerite R, Marioni, Riccardo, Shen, Jincheng, Tsai, Pei-Chien, Montasser, May E, Jia, Yucheng, Syme, Catriona, Salfati, Elias L, Boerwinkle, Eric, Guan, Weihua, Mosley, Thomas H, Bressler, Jan, Morrison, Alanna C, Liu, Chunyu, Mendelson, Michael M, Uitterlinden, André G, van Meurs, Joyce B, Consortium, BIOS, Heijmans, Bastiaan T, Hoen, Peter AC ’t, van Meurs, Joyce, Isaacs, Aaron, Jansen, Rick, Franke, Lude, Boomsma, Dorret I, Pool, René, van Dongen, Jenny, Hottenga, Jouke J, van Greevenbroek, Marleen MJ, Stehouwer, Coen DA, van der Kallen, Carla JH, Schalkwijk, Casper G, Wijmenga, Cisca, Zhernakova, Alexandra, Tigchelaar, Ettje F, Slagboom, P Eline, Beekman, Marian, Deelen, Joris, van Heemst, Diana, Veldink, Jan H, van den Berg, Leonard H, van Duijn, Cornelia M, Hofman, Albert, Jhamai, P Mila, Verbiest, Michael, Suchiman, H Eka D, Verkerk, Marijn, van der Breggen, Ruud, van Rooij, Jeroen, Lakenberg, Nico, Mei, Hailiang, van Iterson, Maarten, van Galen, Michiel, Bot, Jan, van ’t Hof, Peter, Deelen, Patrick, Nooren, Irene, Moed, Matthijs, Vermaat, Martijn, Zhernakova, Dasha V, Luijk, René, Bonder, Marc Jan, van Dijk, Freerk, Arindrarto, Wibowo, Kielbasa, Szymon M, Swertz, Morris A, van Zwet, Erik W, Franco, Oscar H, Zhang, Guosheng, Li, Yun, Stewart, James D, Bis, Joshua C, Psaty, Bruce M, Chen, Yii-Der Ida, Kardia, Sharon LR, Zhao, Wei, Turner, Stephen T, Absher, Devin, Aslibekyan, Stella, Starr, John M, McRae, Allan F, Hou, Lifang, Just, Allan C, Schwartz, Joel D, Vokonas, Pantel S, Menni, Cristina, Spector, Tim D, Shuldiner, Alan, Damcott, Coleen M, Rotter, Jerome I, Palmas, Walter, Liu, Yongmei, and Paus, Tomáš
- Subjects
Epidemiology ,Biological Sciences ,Health Sciences ,Genetics ,Human Genome ,Biotechnology ,Aetiology ,2.1 Biological and endogenous factors ,Aged ,Blood Pressure ,CpG Islands ,Cross-Sectional Studies ,DNA Methylation ,Epigenesis ,Genetic ,Genetic Variation ,Genome-Wide Association Study ,Humans ,Mendelian Randomization Analysis ,Middle Aged ,Nerve Tissue Proteins ,Quantitative Trait Loci ,Tetraspanins ,BIOS Consortium ,DNA methylation ,Mendelian randomization ,blood pressure ,epigenome-wide association study ,gene expression ,sequence variation ,Medical and Health Sciences ,Genetics & Heredity ,Biological sciences ,Biomedical and clinical sciences ,Health sciences - Abstract
Genome-wide association studies have identified hundreds of genetic variants associated with blood pressure (BP), but sequence variation accounts for a small fraction of the phenotypic variance. Epigenetic changes may alter the expression of genes involved in BP regulation and explain part of the missing heritability. We therefore conducted a two-stage meta-analysis of the cross-sectional associations of systolic and diastolic BP with blood-derived genome-wide DNA methylation measured on the Infinium HumanMethylation450 BeadChip in 17,010 individuals of European, African American, and Hispanic ancestry. Of 31 discovery-stage cytosine-phosphate-guanine (CpG) dinucleotides, 13 replicated after Bonferroni correction (discovery: N = 9,828, p < 1.0 × 10-7; replication: N = 7,182, p 30%) and independent of known BP genetic variants, explaining an additional 1.4% and 2.0% of the interindividual variation in systolic and diastolic BP, respectively. Bidirectional Mendelian randomization among up to 4,513 individuals of European ancestry from 4 cohorts suggested that methylation at cg08035323 (TAF1B-YWHAQ) influences BP, while BP influences methylation at cg00533891 (ZMIZ1), cg00574958 (CPT1A), and cg02711608 (SLC1A5). Gene expression analyses further identified six genes (TSPAN2, SLC7A11, UNC93B1, CPT1A, PTMS, and LPCAT3) with evidence of triangular associations between methylation, gene expression, and BP. Additional integrative Mendelian randomization analyses of gene expression and DNA methylation suggested that the expression of TSPAN2 is a putative mediator of association between DNA methylation at cg23999170 and BP. These findings suggest that heritable DNA methylation plays a role in regulating BP independently of previously known genetic variants.
- Published
- 2017
9. Exposure to Low Levels of Lead in Utero and Umbilical Cord Blood DNA Methylation in Project Viva: An Epigenome-Wide Association Study.
- Author
-
Wu, Shaowei, Hivert, Marie-France, Cardenas, Andres, Zhong, Jia, Rifas-Shiman, Sheryl L, Agha, Golareh, Colicino, Elena, Just, Allan C, Amarasiriwardena, Chitra, Lin, Xihong, Litonjua, Augusto A, DeMeo, Dawn L, Gillman, Matthew W, Wright, Robert O, Oken, Emily, and Baccarelli, Andrea A
- Subjects
Erythrocytes ,Fetal Blood ,Humans ,Prenatal Exposure Delayed Effects ,Lead ,Environmental Pollutants ,Prospective Studies ,Maternal Exposure ,DNA Methylation ,Pregnancy ,Adult ,Child ,Infant ,Newborn ,Female ,Male ,Genome-Wide Association Study ,Infant ,Newborn ,Toxicology ,Environmental Sciences ,Medical and Health Sciences - Abstract
BackgroundEarly-life exposure to lead is associated with deficits in neurodevelopment and with hematopoietic system toxicity. DNA methylation may be one of the underlying mechanisms for the adverse effects of prenatal lead on the offspring, but epigenome-wide methylation data for low levels of prenatal lead exposure are lacking.ObjectivesWe investigated the association between prenatal maternal lead exposure and epigenome-wide DNA methylation in umbilical cord blood nucleated cells in Project Viva, a prospective U.S.-based prebirth cohort with relatively low levels of lead exposure.MethodsAmong 268 mother-infant pairs, we measured lead concentrations in red blood cells (RBC) from prenatal maternal blood samples, and using HumanMethylation450 Bead Chips, we measured genome-wide methylation levels at 482,397 CpG loci in umbilical cord blood and retained 394,460 loci after quality control. After adjustment for batch effects, cell types, and covariates, we used robust linear regression models to examine associations of prenatal lead exposure with DNA methylation in cord blood at epigenome-wide significance level [false discovery rate (FDR)
- Published
- 2017
10. An epigenetic clock for gestational age at birth based on blood methylation data
- Author
-
Knight, Anna K, Craig, Jeffrey M, Theda, Christiane, Bækvad-Hansen, Marie, Bybjerg-Grauholm, Jonas, Hansen, Christine S, Hollegaard, Mads V, Hougaard, David M, Mortensen, Preben B, Weinsheimer, Shantel M, Werge, Thomas M, Brennan, Patricia A, Cubells, Joseph F, Newport, D Jeffrey, Stowe, Zachary N, Cheong, Jeanie LY, Dalach, Philippa, Doyle, Lex W, Loke, Yuk J, Baccarelli, Andrea A, Just, Allan C, Wright, Robert O, Téllez-Rojo, Mara M, Svensson, Katherine, Trevisi, Letizia, Kennedy, Elizabeth M, Binder, Elisabeth B, Iurato, Stella, Czamara, Darina, Räikkönen, Katri, Lahti, Jari MT, Pesonen, Anu-Katriina, Kajantie, Eero, Villa, Pia M, Laivuori, Hannele, Hämäläinen, Esa, Park, Hea Jin, Bailey, Lynn B, Parets, Sasha E, Kilaru, Varun, Menon, Ramkumar, Horvath, Steve, Bush, Nicole R, LeWinn, Kaja Z, Tylavsky, Frances A, Conneely, Karen N, and Smith, Alicia K
- Subjects
Biological Sciences ,Biomedical and Clinical Sciences ,Genetics ,Infant Mortality ,Clinical Research ,Preterm ,Low Birth Weight and Health of the Newborn ,Perinatal Period - Conditions Originating in Perinatal Period ,Pediatric ,4.2 Evaluation of markers and technologies ,Detection ,screening and diagnosis ,Reproductive health and childbirth ,Generic health relevance ,Adult ,Aging ,Biomarkers ,Birth Weight ,CpG Islands ,DNA Methylation ,Epigenesis ,Genetic ,Female ,Fetal Development ,Gestational Age ,Humans ,Infant ,Newborn ,Male ,Pregnancy ,Developmental age ,Epigenetic clock ,DNA methylation ,Preterm birth ,Cord blood ,Fetus ,Blood spot ,Biomarker ,Medicaid ,Socioeconomic status ,Birthweight ,Environmental Sciences ,Information and Computing Sciences ,Bioinformatics - Abstract
BackgroundGestational age is often used as a proxy for developmental maturity by clinicians and researchers alike. DNA methylation has previously been shown to be associated with age and has been used to accurately estimate chronological age in children and adults. In the current study, we examine whether DNA methylation in cord blood can be used to estimate gestational age at birth.ResultsWe find that gestational age can be accurately estimated from DNA methylation of neonatal cord blood and blood spot samples. We calculate a DNA methylation gestational age using 148 CpG sites selected through elastic net regression in six training datasets. We evaluate predictive accuracy in nine testing datasets and find that the accuracy of the DNA methylation gestational age is consistent with that of gestational age estimates based on established methods, such as ultrasound. We also find that an increased DNA methylation gestational age relative to clinical gestational age is associated with birthweight independent of gestational age, sex, and ancestry.ConclusionsDNA methylation can be used to accurately estimate gestational age at or near birth and may provide additional information relevant to developmental stage. Further studies of this predictor are warranted to determine its utility in clinical settings and for research purposes. When clinical estimates are available this measure may increase accuracy in the testing of hypotheses related to developmental age and other early life circumstances.
- Published
- 2016
11. DNA methylation-based measures of biological age: meta-analysis predicting time to death
- Author
-
Chen, Brian H, Marioni, Riccardo E, Colicino, Elena, Peters, Marjolein J, Ward-Caviness, Cavin K, Tsai, Pei-Chien, Roetker, Nicholas S, Just, Allan C, Demerath, Ellen W, Guan, Weihua, Bressler, Jan, Fornage, Myriam, Studenski, Stephanie, Vandiver, Amy R, Moore, Ann Zenobia, Tanaka, Toshiko, Kiel, Douglas P, Liang, Liming, Vokonas, Pantel, Schwartz, Joel, Lunetta, Kathryn L, Murabito, Joanne M, Bandinelli, Stefania, Hernandez, Dena G, Melzer, David, Nalls, Michael, Pilling, Luke C, Price, Timothy R, Singleton, Andrew B, Gieger, Christian, Holle, Rolf, Kretschmer, Anja, Kronenberg, Florian, Kunze, Sonja, Linseisen, Jakob, Meisinger, Christine, Rathmann, Wolfgang, Waldenberger, Melanie, Visscher, Peter M, Shah, Sonia, Wray, Naomi R, McRae, Allan F, Franco, Oscar H, Hofman, Albert, Uitterlinden, André G, Absher, Devin, Assimes, Themistocles, Levine, Morgan E, Lu, Ake T, Tsao, Philip S, Hou, Lifang, Manson, JoAnn E, Carty, Cara L, LaCroix, Andrea Z, Reiner, Alexander P, Spector, Tim D, Feinberg, Andrew P, Levy, Daniel, Baccarelli, Andrea, van Meurs, Joyce, Bell, Jordana T, Peters, Annette, Deary, Ian J, Pankow, James S, Ferrucci, Luigi, and Horvath, Steve
- Subjects
Biological Sciences ,Genetics ,Clinical Research ,Prevention ,Good Health and Well Being ,Aging ,DNA Methylation ,Epigenesis ,Genetic ,Female ,Humans ,Logistic Models ,Male ,Mortality ,Racial Groups ,Risk Factors ,Survival Analysis ,T-Lymphocyte Subsets ,all-cause mortality ,lifespan ,epigenetics ,epigenetic clock ,DNA methylation ,mortality ,Biochemistry and cell biology ,Clinical sciences - Abstract
Estimates of biological age based on DNA methylation patterns, often referred to as "epigenetic age", "DNAm age", have been shown to be robust biomarkers of age in humans. We previously demonstrated that independent of chronological age, epigenetic age assessed in blood predicted all-cause mortality in four human cohorts. Here, we expanded our original observation to 13 different cohorts for a total sample size of 13,089 individuals, including three racial/ethnic groups. In addition, we examined whether incorporating information on blood cell composition into the epigenetic age metrics improves their predictive power for mortality. All considered measures of epigenetic age acceleration were predictive of mortality (p≤8.2x10-9), independent of chronological age, even after adjusting for additional risk factors (p
- Published
- 2016
12. DNA Methylation in Newborns and Maternal Smoking in Pregnancy: Genome-wide Consortium Meta-analysis
- Author
-
Joubert, Bonnie R, Felix, Janine F, Yousefi, Paul, Bakulski, Kelly M, Just, Allan C, Breton, Carrie, Reese, Sarah E, Markunas, Christina A, Richmond, Rebecca C, Xu, Cheng-Jian, Küpers, Leanne K, Oh, Sam S, Hoyo, Cathrine, Gruzieva, Olena, Söderhäll, Cilla, Salas, Lucas A, Baïz, Nour, Zhang, Hongmei, Lepeule, Johanna, Ruiz, Carlos, Ligthart, Symen, Wang, Tianyuan, Taylor, Jack A, Duijts, Liesbeth, Sharp, Gemma C, Jankipersadsing, Soesma A, Nilsen, Roy M, Vaez, Ahmad, Fallin, M Daniele, Hu, Donglei, Litonjua, Augusto A, Fuemmeler, Bernard F, Huen, Karen, Kere, Juha, Kull, Inger, Munthe-Kaas, Monica Cheng, Gehring, Ulrike, Bustamante, Mariona, Saurel-Coubizolles, Marie José, Quraishi, Bilal M, Ren, Jie, Tost, Jörg, Gonzalez, Juan R, Peters, Marjolein J, Håberg, Siri E, Xu, Zongli, van Meurs, Joyce B, Gaunt, Tom R, Kerkhof, Marjan, Corpeleijn, Eva, Feinberg, Andrew P, Eng, Celeste, Baccarelli, Andrea A, Neelon, Sara E Benjamin, Bradman, Asa, Merid, Simon Kebede, Bergström, Anna, Herceg, Zdenko, Hernandez-Vargas, Hector, Brunekreef, Bert, Pinart, Mariona, Heude, Barbara, Ewart, Susan, Yao, Jin, Lemonnier, Nathanaël, Franco, Oscar H, Wu, Michael C, Hofman, Albert, McArdle, Wendy, Van der Vlies, Pieter, Falahi, Fahimeh, Gillman, Matthew W, Barcellos, Lisa F, Kumar, Ashish, Wickman, Magnus, Guerra, Stefano, Charles, Marie-Aline, Holloway, John, Auffray, Charles, Tiemeier, Henning W, Smith, George Davey, Postma, Dirkje, Hivert, Marie-France, Eskenazi, Brenda, Vrijheid, Martine, Arshad, Hasan, Antó, Josep M, Dehghan, Abbas, Karmaus, Wilfried, Annesi-Maesano, Isabella, Sunyer, Jordi, Ghantous, Akram, Pershagen, Göran, Holland, Nina, Murphy, Susan K, DeMeo, Dawn L, Burchard, Esteban G, Ladd-Acosta, Christine, Snieder, Harold, and Nystad, Wenche
- Subjects
Genetics ,Pediatric ,Human Genome ,Tobacco ,Tobacco Smoke and Health ,Prevention ,Aetiology ,2.1 Biological and endogenous factors ,2.2 Factors relating to the physical environment ,Respiratory ,Reproductive health and childbirth ,Good Health and Well Being ,Asthma ,Child ,Child ,Preschool ,Chromosome Mapping ,Cleft Lip ,Cleft Palate ,DNA Methylation ,Epigenesis ,Genetic ,Female ,Genetic Association Studies ,Humans ,Infant ,Infant ,Newborn ,Pregnancy ,Smoking ,White People ,Biological Sciences ,Medical and Health Sciences ,Genetics & Heredity - Abstract
Epigenetic modifications, including DNA methylation, represent a potential mechanism for environmental impacts on human disease. Maternal smoking in pregnancy remains an important public health problem that impacts child health in a myriad of ways and has potential lifelong consequences. The mechanisms are largely unknown, but epigenetics most likely plays a role. We formed the Pregnancy And Childhood Epigenetics (PACE) consortium and meta-analyzed, across 13 cohorts (n = 6,685), the association between maternal smoking in pregnancy and newborn blood DNA methylation at over 450,000 CpG sites (CpGs) by using the Illumina 450K BeadChip. Over 6,000 CpGs were differentially methylated in relation to maternal smoking at genome-wide statistical significance (false discovery rate, 5%), including 2,965 CpGs corresponding to 2,017 genes not previously related to smoking and methylation in either newborns or adults. Several genes are relevant to diseases that can be caused by maternal smoking (e.g., orofacial clefts and asthma) or adult smoking (e.g., certain cancers). A number of differentially methylated CpGs were associated with gene expression. We observed enrichment in pathways and processes critical to development. In older children (5 cohorts, n = 3,187), 100% of CpGs gave at least nominal levels of significance, far more than expected by chance (p value < 2.2 × 10(-16)). Results were robust to different normalization methods used across studies and cell type adjustment. In this large scale meta-analysis of methylation data, we identified numerous loci involved in response to maternal smoking in pregnancy with persistence into later childhood and provide insights into mechanisms underlying effects of this important exposure.
- Published
- 2016
13. DNA methylation age of blood predicts all-cause mortality in later life
- Author
-
Marioni, Riccardo E, Shah, Sonia, McRae, Allan F, Chen, Brian H, Colicino, Elena, Harris, Sarah E, Gibson, Jude, Henders, Anjali K, Redmond, Paul, Cox, Simon R, Pattie, Alison, Corley, Janie, Murphy, Lee, Martin, Nicholas G, Montgomery, Grant W, Feinberg, Andrew P, Fallin, M Daniele, Multhaup, Michael L, Jaffe, Andrew E, Joehanes, Roby, Schwartz, Joel, Just, Allan C, Lunetta, Kathryn L, Murabito, Joanne M, Starr, John M, Horvath, Steve, Baccarelli, Andrea A, Levy, Daniel, Visscher, Peter M, Wray, Naomi R, and Deary, Ian J
- Subjects
Genetics ,Prevention ,Aging ,Cardiovascular ,Heart Disease ,Clinical Research ,Human Genome ,4.1 Discovery and preclinical testing of markers and technologies ,Detection ,screening and diagnosis ,Good Health and Well Being ,Aged ,Blood ,Cause of Death ,Cohort Studies ,DNA Methylation ,Demography ,Female ,Humans ,Inheritance Patterns ,Leukocyte Count ,Male ,Mortality ,Risk Factors ,Survival Analysis ,Environmental Sciences ,Biological Sciences ,Information and Computing Sciences ,Bioinformatics - Abstract
BackgroundDNA methylation levels change with age. Recent studies have identified biomarkers of chronological age based on DNA methylation levels. It is not yet known whether DNA methylation age captures aspects of biological age.ResultsHere we test whether differences between people's chronological ages and estimated ages, DNA methylation age, predict all-cause mortality in later life. The difference between DNA methylation age and chronological age (Δage) was calculated in four longitudinal cohorts of older people. Meta-analysis of proportional hazards models from the four cohorts was used to determine the association between Δage and mortality. A 5-year higher Δage is associated with a 21% higher mortality risk, adjusting for age and sex. After further adjustments for childhood IQ, education, social class, hypertension, diabetes, cardiovascular disease, and APOE e4 status, there is a 16% increased mortality risk for those with a 5-year higher Δage. A pedigree-based heritability analysis of Δage was conducted in a separate cohort. The heritability of Δage was 0.43.ConclusionsDNA methylation-derived measures of accelerated aging are heritable traits that predict mortality independently of health status, lifestyle factors, and known genetic factors.
- Published
- 2015
14. Improved filtering of DNA methylation microarray data by detection p values and its impact on downstream analyses
- Author
-
Heiss, Jonathan A. and Just, Allan C.
- Published
- 2019
- Full Text
- View/download PDF
15. Analysis of repeated leukocyte DNA methylation assessments reveals persistent epigenetic alterations after an incident myocardial infarction
- Author
-
Ward-Caviness, Cavin K., Agha, Golareh, Chen, Brian H., Pfeiffer, Liliane, Wilson, Rory, Wolf, Petra, Gieger, Christian, Schwartz, Joel, Vokonas, Pantel S., Hou, Lifang, Just, Allan C., Bandinelli, Stefania, Hernandez, Dena G., Singleton, Andrew B., Prokisch, Holger, Meitinger, Thomas, Kastenmüller, Gabi, Ferrucci, Luigi, Baccarelli, Andrea A., Waldenberger, Melanie, and Peters, Annette
- Published
- 2018
- Full Text
- View/download PDF
16. Identifying mislabeled and contaminated DNA methylation microarray data: an extended quality control toolset with examples from GEO
- Author
-
Heiss, Jonathan A. and Just, Allan C.
- Published
- 2018
- Full Text
- View/download PDF
17. Multi-Omics Analysis Reveals MicroRNAs Associated With Cardiometabolic Traits
- Author
-
Michelle M. J. Mens, Silvana C. E. Maas, Jaco Klap, Gerrit Jan Weverling, Paul Klatser, Just P. J. Brakenhoff, Joyce B. J. van Meurs, André G. Uitterlinden, M. Arfan Ikram, Maryam Kavousi, Mohsen Ghanbari, Epidemiology, Genetic Identification, and Internal Medicine
- Subjects
0301 basic medicine ,lcsh:QH426-470 ,multi-omics data ,Population ,Genome-wide association study ,Computational biology ,Biology ,03 medical and health sciences ,Rotterdam Study ,0302 clinical medicine ,microRNA ,Genetics ,GWAS ,education ,Genetics (clinical) ,Original Research ,EWAS ,Genetic association ,education.field_of_study ,lcsh:Genetics ,030104 developmental biology ,CpG site ,030220 oncology & carcinogenesis ,DNA methylation ,Trait ,Molecular Medicine ,cardiometabolic traits - Abstract
MicroRNAs (miRNAs) are non-coding RNA molecules that regulate gene expression. Extensive research has explored the role of miRNAs in the risk for type 2 diabetes (T2D) and coronary heart disease (CHD) using single-omics data, but much less by leveraging population-based omics data. Here we aimed to conduct a multi-omics analysis to identify miRNAs associated with cardiometabolic risk factors and diseases. First, we used publicly available summary statistics from large-scale genome-wide association studies to find genetic variants in miRNA-related sequences associated with various cardiometabolic traits, including lipid and obesity-related traits, glycemic indices, blood pressure, and disease prevalence of T2D and CHD. Then, we used DNA methylation and miRNA expression data from participants of the Rotterdam Study to further investigate the link between associated miRNAs and cardiometabolic traits. After correcting for multiple testing, 180 genetic variants annotated to 67 independent miRNAs were associated with the studied traits. Alterations in DNA methylation levels of CpG sites annotated to 38 of these miRNAs were associated with the same trait(s). Moreover, we found that plasma expression levels of 8 of the 67 identified miRNAs were also associated with the same trait. Integrating the results of different omics data showed miR-10b-5p, miR-148a-3p, miR-125b-5p, and miR-100-5p to be strongly linked to lipid traits. Collectively, our multi-omics analysis revealed multiple miRNAs that could be considered as potential biomarkers for early diagnosis and progression of cardiometabolic diseases.
- Published
- 2020
18. Multi-Omics Analysis Reveals MicroRNAs Associated With Cardiometabolic Traits.
- Author
-
Mens, Michelle M. J., Maas, Silvana C. E., Klap, Jaco, Weverling, Gerrit Jan, Klatser, Paul, Brakenhoff, Just P. J., van Meurs, Joyce B. J., Uitterlinden, André G., Ikram, M. Arfan, Kavousi, Maryam, and Ghanbari, Mohsen
- Subjects
DISEASE risk factors ,NON-coding RNA ,HEART metabolism disorders ,TYPE 2 diabetes ,DNA methylation ,GLYCEMIC index - Abstract
MicroRNAs (miRNAs) are non-coding RNA molecules that regulate gene expression. Extensive research has explored the role of miRNAs in the risk for type 2 diabetes (T2D) and coronary heart disease (CHD) using single-omics data, but much less by leveraging population-based omics data. Here we aimed to conduct a multi-omics analysis to identify miRNAs associated with cardiometabolic risk factors and diseases. First, we used publicly available summary statistics from large-scale genome-wide association studies to find genetic variants in miRNA-related sequences associated with various cardiometabolic traits, including lipid and obesity-related traits, glycemic indices, blood pressure, and disease prevalence of T2D and CHD. Then, we used DNA methylation and miRNA expression data from participants of the Rotterdam Study to further investigate the link between associated miRNAs and cardiometabolic traits. After correcting for multiple testing, 180 genetic variants annotated to 67 independent miRNAs were associated with the studied traits. Alterations in DNA methylation levels of CpG sites annotated to 38 of these miRNAs were associated with the same trait(s). Moreover, we found that plasma expression levels of 8 of the 67 identified miRNAs were also associated with the same trait. Integrating the results of different omics data showed miR-10b-5p, miR-148a-3p, miR-125b-5p, and miR-100-5p to be strongly linked to lipid traits. Collectively, our multi-omics analysis revealed multiple miRNAs that could be considered as potential biomarkers for early diagnosis and progression of cardiometabolic diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
19. Epigenetic Signatures of Cigarette Smoking.
- Author
-
Joehanes, Roby, Just, Allan C., Marioni, Riccardo E., Pilling, Luke C., Reynolds, Lindsay M., Mandaviya, Pooja R., Weihua Guan, Tao Xu, Elks, Cathy E., Aslibekyan, Stella, Moreno-Macias, Hortensia, Smith, Jennifer A., Brody, Jennifer A., Dhingra, Radhika, Yousefi, Paul, Pankow, James S., Kunze, Sonja, Shah, Sonia H., McRae, Allan F., and Lohman, Kurt
- Subjects
SMOKING ,SMOKING cessation ,DNA methylation ,GENETICS - Abstract
Background--DNA methylation leaves a long-term signature of smoking exposure and is one potential mechanism by which tobacco exposure predisposes to adverse health outcomes, such as cancers, osteoporosis, lung, and cardiovascular disorders. Methods and Results--To comprehensively determine the association between cigarette smoking and DNA methylation, we conducted a meta-analysis of genome-wide DNA methylation assessed using the Illumina BeadChip 450K array on 15 907 blood-derived DNA samples from participants in 16 cohorts (including 2433 current, 6518 former, and 6956 never smokers). Comparing current versus never smokers, 2623 cytosine-phosphate-guanine sites (CpGs), annotated to 1405 genes, were statistically significantly differentially methylated at Bonferroni threshold of P<1?10
-7 (18 760 CpGs at false discovery rate <0.05). Genes annotated to these CpGs were enriched for associations with several smokingrelated traits in genome-wide studies including pulmonary function, cancers, inflammatory diseases, and heart disease. Comparing former versus never smokers, 185 of the CpGs that differed between current and never smokers were significant P<1?10-7 (2623 CpGs at false discovery rate <0.05), indicating a pattern of persistent altered methylation, with attenuation, after smoking cessation. Transcriptomic integration identified effects on gene expression at many differentially methylated CpGs. Conclusions--Cigarette smoking has a broad impact on genome-wide methylation that, at many loci, persists many years after smoking cessation. Many of the differentially methylated genes were novel genes with respect to biological effects of smoking and might represent therapeutic targets for prevention or treatment of tobacco-related diseases. Methylation at these sites could also serve as sensitive and stable biomarkers of lifetime exposure to tobacco smoke. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
20. DNA methylation-based measures of biological age: meta-analysis predicting time to death
- Author
-
Chen, Brian H., Marioni, Riccardo E., Colicino, Elena, Peters, Marjolein J., Ward-Caviness, Cavin K., Tsai, Pei-Chien, Roetker, Nicholas S., Just, Allan C., Demerath, Ellen W., Guan, Weihua, Bressler, Jan, Fornage, Myriam, Studenski, Stephanie, Vandiver, Amy R., Moore, Ann Zenobia, Tanaka, Toshiko, Kiel, Douglas P., Liang, Liming, Vokonas, Pantel, Schwartz, Joel, Lunetta, Kathryn L., Murabito, Joanne M., Bandinelli, Stefania, Hernandez, Dena G., Melzer, David, Nalls, Michael, Pilling, Luke C., Price, Timothy R., Singleton, Andrew B., Gieger, Christian, Holle, Rolf, Kretschmer, Anja, Kronenberg, Florian, Kunze, Sonja, Linseisen, Jakob, Meisinger, Christine, Rathmann, Wolfgang, Waldenberger, Melanie, Visscher, Peter M., Shah, Sonia, Wray, Naomi R., McRae, Allan F., Franco, Oscar H., Hofman, Albert, Uitterlinden, André G., Absher, Devin, Assimes, Themistocles, Levine, Morgan E., Lu, Ake T., Tsao, Philip S., Hou, Lifang, Manson, JoAnn E., Carty, Cara L., LaCroix, Andrea Z., Reiner, Alexander P., Spector, Tim D., Feinberg, Andrew P., Levy, Daniel, Baccarelli, Andrea, van Meurs, Joyce, Bell, Jordana T., Peters, Annette, Deary, Ian J., Pankow, James S., Ferrucci, Luigi, and Horvath, Steve
- Subjects
all-cause mortality ,lifespan ,epigenetics ,epigenetic clock ,DNA methylation ,mortality - Abstract
Estimates of biological age based on DNA methylation patterns, often referred to as “epigenetic age”, “DNAm age”, have been shown to be robust biomarkers of age in humans. We previously demonstrated that independent of chronological age, epigenetic age assessed in blood predicted all-cause mortality in four human cohorts. Here, we expanded our original observation to 13 different cohorts for a total sample size of 13,089 individuals, including three racial/ethnic groups. In addition, we examined whether incorporating information on blood cell composition into the epigenetic age metrics improves their predictive power for mortality. All considered measures of epigenetic age acceleration were predictive of mortality (p≤8.2×10−9), independent of chronological age, even after adjusting for additional risk factors (p<5.4×10−4), and within the racial/ethnic groups that we examined (non-Hispanic whites, Hispanics, African Americans). Epigenetic age estimates that incorporated information on blood cell composition led to the smallest p-values for time to death (p=7.5×10−43). Overall, this study a) strengthens the evidence that epigenetic age predicts all-cause mortality above and beyond chronological age and traditional risk factors, and b) demonstrates that epigenetic age estimates that incorporate information on blood cell counts lead to highly significant associations with all-cause mortality.
- Published
- 2016
- Full Text
- View/download PDF
21. An epigenetic clock for gestational age at birth based on blood methylation data
- Author
-
Knight, Anna K., Craig, Jeffrey M., Theda, Christiane, Bækvad-Hansen, Marie, Bybjerg-Grauholm, Jonas, Hansen, Christine S., Hollegaard, Mads V., Hougaard, David M., Mortensen, Preben B., Weinsheimer, Shantel M., Werge, Thomas M., Brennan, Patricia A., Cubells, Joseph F., Newport, D. Jeffrey, Stowe, Zachary N., Cheong, Jeanie L. Y., Dalach, Philippa, Doyle, Lex W., Loke, Yuk J., Baccarelli, Andrea A., Just, Allan C., Wright, Robert O., Téllez-Rojo, Mara M., Svensson, Katherine, Trevisi, Letizia, Kennedy, Elizabeth M., Binder, Elisabeth B., Iurato, Stella, Czamara, Darina, Räikkönen, Katri, Lahti, Jari M. T., Pesonen, Anu-Katriina, Kajantie, Eero, Villa, Pia M., Laivuori, Hannele, Hämäläinen, Esa, Park, Hea Jin, Bailey, Lynn B., Parets, Sasha E., Kilaru, Varun, Menon, Ramkumar, Horvath, Steve, Bush, Nicole R., LeWinn, Kaja Z., Tylavsky, Frances A., Conneely, Karen N., and Smith, Alicia K.
- Subjects
Developmental age ,Aging ,Epigenetic clock ,DNA methylation ,Preterm birth ,Cord blood ,Fetus ,Blood spot ,Biomarker ,Medicaid ,Socioeconomic status ,Birthweight - Abstract
Background: Gestational age is often used as a proxy for developmental maturity by clinicians and researchers alike. DNA methylation has previously been shown to be associated with age and has been used to accurately estimate chronological age in children and adults. In the current study, we examine whether DNA methylation in cord blood can be used to estimate gestational age at birth. Results: We find that gestational age can be accurately estimated from DNA methylation of neonatal cord blood and blood spot samples. We calculate a DNA methylation gestational age using 148 CpG sites selected through elastic net regression in six training datasets. We evaluate predictive accuracy in nine testing datasets and find that the accuracy of the DNA methylation gestational age is consistent with that of gestational age estimates based on established methods, such as ultrasound. We also find that an increased DNA methylation gestational age relative to clinical gestational age is associated with birthweight independent of gestational age, sex, and ancestry. Conclusions: DNA methylation can be used to accurately estimate gestational age at or near birth and may provide additional information relevant to developmental stage. Further studies of this predictor are warranted to determine its utility in clinical settings and for research purposes. When clinical estimates are available this measure may increase accuracy in the testing of hypotheses related to developmental age and other early life circumstances. Electronic supplementary material The online version of this article (doi:10.1186/s13059-016-1068-z) contains supplementary material, which is available to authorized users.
- Published
- 2016
- Full Text
- View/download PDF
22. Birth weight-for-gestational age is associated with DNA methylation at birth and in childhood
- Author
-
Agha, Golareh, Hajj, Hanine, Rifas-Shiman, Sheryl L., Just, Allan C., Hivert, Marie-France, Burris, Heather H., Lin, Xihong, Litonjua, Augusto A., Oken, Emily, DeMeo, Dawn L., Gillman, Matthew W., and Baccarelli, Andrea A.
- Subjects
Epigenetics ,DNA methylation ,Birth weight - Abstract
Background: Both higher and lower fetal growth are associated with cardio-metabolic health later in life, suggesting that prenatal developmental programming determines long-term cardiovascular disease risk. Epigenetic mechanisms, which orchestrate fetal growth and development, may offer insight on the early programming of health and disease. We investigated whether birth weight-for-gestational is associated with DNA methylation at birth and mid-childhood, measured via the Infinium 450K array. Methods/results Participants were from Project Viva, a pre-birth cohort of pregnant women and their children in Eastern Massachusetts. After exclusion of participants with maternal type 1 or 2 diabetes and gestational age <34 weeks, we used DNA methylation assays from 476 venous umbilical cord blood samples and a subset of 235 who additionally had peripheral blood samples available in mid-childhood (age 7–10 years). Among 392,918 CpG sites analyzed, birth weight-for-gestational age z-score was associated with cord blood DNA methylation at 34 CpGs (false discovery rate P < 0.05), after adjusting for maternal age, race/ethnicity, education, smoking, parity, delivery mode, pre-pregnancy BMI, gestational diabetes status, child sex, and estimated cord blood cell proportions based on a cord blood reference panel. Two of these CpGs were previously reported in epigenome-wide analyses of birth weight, and several other CpGs map to genes relevant to fetal growth and development. Namely, higher birth weight-for-gestational age was associated with higher methylation at four CpGs at the PBX1 locus (e.g., β (95% CI) for lead signal at cg06750897 = 1.9 (1.2, 2.6)), which encodes a transcription factor that regulates embryonic development. Birth weight-for-gestational age was also associated with mid-childhood blood DNA methylation at four of the 34 CpGs identified in cord blood analyses, including sites at the PBX1 locus described. Conclusions: We identified CpG sites where birth weight-for-gestational age was associated with DNA methylation at birth, and for a subset of these sites, birth weight-for-gestational age was also associated with DNA methylation at mid-childhood. Electronic supplementary material The online version of this article (doi:10.1186/s13148-016-0285-3) contains supplementary material, which is available to authorized users.
- Published
- 2016
- Full Text
- View/download PDF
23. DNA methylation signatures of chronic low-grade inflammation are associated with complex diseases
- Author
-
Ligthart, Symen, Marzi, Carola, Aslibekyan, Stella, Mendelson, Michael M., Conneely, Karen N., Tanaka, Toshiko, Colicino, Elena, Waite, Lindsay L., Joehanes, Roby, Guan, Weihua, Brody, Jennifer A., Elks, Cathy, Marioni, Riccardo, Jhun, Min A., Agha, Golareh, Bressler, Jan, Ward-Caviness, Cavin K., Chen, Brian H., Huan, Tianxiao, Bakulski, Kelly, Salfati, Elias L., Fiorito, Giovanni, Wahl, Simone, Schramm, Katharina, Sha, Jin, Hernandez, Dena G., Just, Allan C., Smith, Jennifer A., Sotoodehnia, Nona, Pilling, Luke C., Pankow, James S., Tsao, Phil S., Liu, Chunyu, Zhao, Wei, Guarrera, Simonetta, Michopoulos, Vasiliki J., Smith, Alicia K., Peters, Marjolein J., Melzer, David, Vokonas, Pantel, Fornage, Myriam, Prokisch, Holger, Bis, Joshua C., Chu, Audrey Y., Herder, Christian, Grallert, Harald, Yao, Chen, Shah, Sonia, McRae, Allan F., Lin, Honghuang, Horvath, Steve, Fallin, Daniele, Hofman, Albert, Wareham, Nicholas J., Wiggins, Kerri L., Feinberg, Andrew P., Starr, John M., Visscher, Peter M., Murabito, Joanne M., Kardia, Sharon L. R., Absher, Devin M., Binder, Elisabeth B., Singleton, Andrew B., Bandinelli, Stefania, Peters, Annette, Waldenberger, Melanie, Matullo, Giuseppe, Schwartz, Joel D., Demerath, Ellen W., Uitterlinden, André G., van Meurs, Joyce B. J., Franco, Oscar H., Chen, Yii-Der Ida, Levy, Daniel, Turner, Stephen T., Deary, Ian J., Ressler, Kerry J., Dupuis, Josée, Ferrucci, Luigi, Ong, Ken K., Assimes, Themistocles L., Boerwinkle, Eric, Koenig, Wolfgang, Arnett, Donna K., Baccarelli, Andrea A., Benjamin, Emelia J., and Dehghan, Abbas
- Subjects
Inflammation ,DNA methylation ,Epigenome-wide association study ,C-reactive protein ,Body mass index ,Diabetes ,Coronary heart disease - Abstract
Background: Chronic low-grade inflammation reflects a subclinical immune response implicated in the pathogenesis of complex diseases. Identifying genetic loci where DNA methylation is associated with chronic low-grade inflammation may reveal novel pathways or therapeutic targets for inflammation. Results: We performed a meta-analysis of epigenome-wide association studies (EWAS) of serum C-reactive protein (CRP), which is a sensitive marker of low-grade inflammation, in a large European population (n = 8863) and trans-ethnic replication in African Americans (n = 4111). We found differential methylation at 218 CpG sites to be associated with CRP (P < 1.15 × 10–7) in the discovery panel of European ancestry and replicated (P < 2.29 × 10–4) 58 CpG sites (45 unique loci) among African Americans. To further characterize the molecular and clinical relevance of the findings, we examined the association with gene expression, genetic sequence variants, and clinical outcomes. DNA methylation at nine (16%) CpG sites was associated with whole blood gene expression in cis (P < 8.47 × 10–5), ten (17%) CpG sites were associated with a nearby genetic variant (P < 2.50 × 10–3), and 51 (88%) were also associated with at least one related cardiometabolic entity (P < 9.58 × 10–5). An additive weighted score of replicated CpG sites accounted for up to 6% inter-individual variation (R2) of age-adjusted and sex-adjusted CRP, independent of known CRP-related genetic variants. Conclusion: We have completed an EWAS of chronic low-grade inflammation and identified many novel genetic loci underlying inflammation that may serve as targets for the development of novel therapeutic interventions for inflammation. Electronic supplementary material The online version of this article (doi:10.1186/s13059-016-1119-5) contains supplementary material, which is available to authorized users.
- Published
- 2016
- Full Text
- View/download PDF
24. Long-term exposure to ambient fine particulate components and leukocyte epigenome-wide DNA Methylation in older men: the Normative Aging Study
- Author
-
Cuicui Wang, Heresh Amini, Zongli Xu, Adjani A. Peralta, Mahdieh Danesh Yazdi, Xinye Qiu, Yaguang Wei, Allan Just, Jonathan Heiss, Lifang Hou, Yinan Zheng, Brent A. Coull, Anna Kosheleva, Andrea A. Baccarelli, and Joel D. Schwartz
- Subjects
PM2.5 components ,Sources ,DNA methylation ,Epigenome-wide association study ,Pathway analyses ,Industrial medicine. Industrial hygiene ,RC963-969 ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background Epigenome-wide association studies of ambient fine particulate matter (PM2.5) have been reported. However, few have examined PM2.5 components (PMCs) and sources or included repeated measures. The lack of high-resolution exposure measurements is the key limitation. We hypothesized that significant changes in DNA methylation might vary by PMCs and the sources. Methods We predicted the annual average of 14 PMCs using novel high-resolution exposure models across the contiguous U.S., between 2000–2018. The resolution was 50 m × 50 m in the Greater Boston Area. We also identified PM2.5 sources using positive matrix factorization. We repeatedly collected blood samples and measured leukocyte DNAm with the Illumina HumanMethylation450K BeadChip in the Normative Aging Study. We then used median regression with subject-specific intercepts to estimate the associations between long-term (one-year) exposure to PMCs / PM2.5 sources and DNA methylation at individual cytosine-phosphate-guanine CpG sites. Significant probes were identified by the number of independent degrees of freedom approach, using the number of principal components explaining > 95% of the variation of the DNA methylation data. We also performed regional and pathway analyses to identify significant regions and pathways. Results We included 669 men with 1,178 visits between 2000–2013. The subjects had a mean age of 75 years. The identified probes, regions, and pathways varied by PMCs and their sources. For example, iron was associated with 6 probes and 6 regions, whereas nitrate was associated with 15 probes and 3 regions. The identified pathways from biomass burning, coal burning, and heavy fuel oil combustion sources were associated with cancer, inflammation, and cardiovascular diseases, whereas there were no pathways associated with all traffic. Conclusions Our findings showed that the effects of PM2.5 on DNAm varied by its PMCs and sources.
- Published
- 2023
- Full Text
- View/download PDF
25. X chromosome dosage and the genetic impact across human tissues
- Author
-
Mette Viuff, Anne Skakkebæk, Emma B. Johannsen, Simon Chang, Steen Bønlykke Pedersen, Katrine Meyer Lauritsen, Mette Glavind Bülow Pedersen, Christian Trolle, Jesper Just, and Claus H. Gravholt
- Subjects
Sex chromosome aneuploidies ,DNA methylation ,RNA expression ,Epigenetics ,Genetics ,Medicine ,QH426-470 - Abstract
Abstract Background Sex chromosome aneuploidies (SCAs) give rise to a broad range of phenotypic traits and diseases. Previous studies based on peripheral blood samples have suggested the presence of ripple effects, caused by altered X chromosome number, affecting the methylome and transcriptome. Whether these alterations can be connected to disease-specific tissues, and thereby having clinical implication for the phenotype, remains to be elucidated. Methods We performed a comprehensive analysis of X chromosome number on the transcriptome and methylome in blood, fat, and muscle tissue from individuals with 45,X, 46,XX, 46,XY, and 47,XXY. Results X chromosome number affected the transcriptome and methylome globally across all chromosomes in a tissue-specific manner. Furthermore, 45,X and 47,XXY demonstrated a divergent pattern of gene expression and methylation, with overall gene downregulation and hypomethylation in 45,X and gene upregulation and hypermethylation in 47,XXY. In fat and muscle, a pronounced effect of sex was observed. We identified X chromosomal genes with an expression pattern different from what would be expected based on the number of X and Y chromosomes. Our data also indicate a regulatory function of Y chromosomal genes on X chromosomal genes. Fourteen X chromosomal genes were downregulated in 45,X and upregulated in 47,XXY, respectively, in all three tissues (AKAP17A, CD99, DHRSX, EIF2S3, GTPBP6, JPX, KDM6A, PP2R3B, PUDP, SLC25A6, TSIX, XIST, ZBED1, ZFX). These genes may be central in the epigenetic and genomic regulation of sex chromosome aneuploidies. Conclusion We highlight a tissue-specific and complex effect of X chromosome number on the transcriptome and methylome, elucidating both shared and non-shared gene-regulatory mechanism between SCAs.
- Published
- 2023
- Full Text
- View/download PDF
26. Associations between infant sex and DNA methylation across umbilical cord blood, artery, and placenta samples
- Author
-
Anne K. Bozack, Elena Colicino, Allan C. Just, Robert O. Wright, Andrea A. Baccarelli, Rosalind J. Wright, and Alison G. Lee
- Subjects
epigenetics ,dna methylation ,ewas ,sex ,birth cohort ,Genetics ,QH426-470 - Abstract
DNA methylation (DNAm) is vulnerable to dysregulation by environmental exposures during epigenetic reprogramming that occurs in embryogenesis. Sexual dimorphism in environmentally induced DNAm dysregulation has been identified and therefore it is important to understand sex-specific DNAm patterns. DNAm at several autosomal sites has been consistently associated with sex in cord blood and placental foetal tissues. However, there is limited research comparing sex-specific DNAm across tissues, particularly differentially methylated regions (DMRs). This study leverages DNAm data measured using the Illumina HumanMethylation450 BeadChip in cord blood (N = 179), placenta (N = 229), and umbilical artery samples (N = 229) in the PRogramming of Intergenerational Stress Mechanisms (PRISM) cohort to identify autosomal DMRs and differentially methylated positions (DMPs). A replication analyses was conducted in an independent cohort (GEO Accession GSE129841). We identified 183, 257, and 419 DMRs and 2119, 2281, and 3405 DMPs (pBonferroni < 0.05) in cord blood, placenta, and artery samples, respectively. Thirty-nine DMRs overlapped in all three tissues, overlapping with genes involved in spermatogenesis (NKAPL, PIWIL2 and AURKC) and X–inactivation (LRIF1). In replication analysis, 85% of DMRs overlapped with those identified in PRISM. Overall, DMRs and DMPs had higher methylation levels among females in cord blood and artery samples, but higher methylation levels among males in placenta samples. Further research is necessary to understand biological mechanisms that contribute to differences in sex-specific DNAm signatures across tissues, as well as to determine if sexual dimorphism in the epigenome impacts response to environmental stressors.
- Published
- 2022
- Full Text
- View/download PDF
27. Saliva cell type DNA methylation reference panel for epidemiological studies in children
- Author
-
Lauren Y. M. Middleton, John Dou, Jonah Fisher, Jonathan A. Heiss, Vy K. Nguyen, Allan C. Just, Jessica Faul, Erin B. Ware, Colter Mitchell, Justin A. Colacino, and Kelly M. Bakulski
- Subjects
dna methylation ,saliva ,epigenetics ,cell type reference ,cellular heterogeneity ,Genetics ,QH426-470 - Abstract
Saliva is a widely used biological sample, especially in pediatric research, containing a heterogenous mixture of immune and epithelial cells. Associations of exposure or disease with saliva DNA methylation can be influenced by cell-type proportions. Here, we developed a saliva cell-type DNA methylation reference panel to estimate interindividual cell-type heterogeneity in whole saliva studies. Saliva was collected from 22 children (7–16 years) and sorted into immune and epithelial cells, using size exclusion filtration and magnetic bead sorting. DNA methylation was measured using the Illumina MethylationEPIC BeadChip. We assessed cell-type differences in DNA methylation profiles and tested for enriched biological pathways. Immune and epithelial cells differed at 181,577 (22.8%) DNA methylation sites (t-test p
- Published
- 2022
- Full Text
- View/download PDF
28. Maternal haemoglobin levels in pregnancy and child DNA methylation: a study in the pregnancy and childhood epigenetics consortium
- Author
-
Justiina Ronkainen, Anni Heiskala, Florianne O.L. Vehmeijer, Estelle Lowry, Doretta Caramaschi, Guadalupe Estrada Gutierrez, Jonathan A. Heiss, Nadine Hummel, Elina Keikkala, Tuomas Kvist, Allison Kupsco, Phillip E. Melton, Giancarlo Pesce, Munawar H. Soomro, Marta Vives-Usano, Nour Baiz, Elisabeth Binder, Darina Czamara, Mònica Guxens, Sanna Mustaniemi, Stephanie J. London, Sebastian Rauschert, Marja Vääräsmäki, Martine Vrijheid, Anette-G. Ziegler, Isabella Annesi-Maesano, Mariona Bustamante, Rae-Chi Huang, Sandra Hummel, Allan C. Just, Eero Kajantie, Jari Lahti, Deborah Lawlor, Katri Räikkönen, Marjo-Riitta Järvelin, Janine F. Felix, and Sylvain Sebert
- Subjects
maternal haemoglobin ,dna methylation ,developmental programming ,pregnancy ,Genetics ,QH426-470 - Abstract
Altered maternal haemoglobin levels during pregnancy are associated with pre-clinical and clinical conditions affecting the fetus. Evidence from animal models suggests that these associations may be partially explained by differential DNA methylation in the newborn with possible long-term consequences. To test this in humans, we meta-analyzed the epigenome-wide associations of maternal haemoglobin levels during pregnancy with offspring DNA methylation in 3,967 newborn cord blood and 1,534 children and 1,962 adolescent whole-blood samples derived from 10 cohorts. DNA methylation was measured using Illumina Infinium Methylation 450K or MethylationEPIC arrays covering 450,000 and 850,000 methylation sites, respectively. There was no statistical support for the association of maternal haemoglobin levels with offspring DNA methylation either at individual methylation sites or clustered in regions. For most participants, maternal haemoglobin levels were within the normal range in the current study, whereas adverse perinatal outcomes often arise at the extremes. Thus, this study does not rule out the possibility that associations with offspring DNA methylation might be seen in studies with more extreme maternal haemoglobin levels.
- Published
- 2022
- Full Text
- View/download PDF
29. Battle of epigenetic proportions: comparing Illumina’s EPIC methylation microarrays and TruSeq targeted bisulfite sequencing
- Author
-
Jonathan A. Heiss, Kasey J. Brennan, Andrea A. Baccarelli, Martha M. Téllez-Rojo, Guadalupe Estrada-Gutiérrez, Robert O. Wright, and Allan C. Just
- Subjects
dna methylation ,ewas ,microarray ,bisulphite sequencing ,comparison of experimental platforms ,Genetics ,QH426-470 - Abstract
DNA methylation microarrays have been the platform of choice for epigenome-wide association studies in epidemiology, but declining costs have rendered targeted bisulphite sequencing a feasible alternative. Nonetheless, the literature for researchers seeking guidance on which platform to choose is sparse. To fill this gap, we conducted a comparison study in which we processed cord blood samples from four newborns in duplicates using both the Illumina HumanMethylationEPIC BeadChip and the Illumina TruSeq Methyl Capture EPIC Kit, and evaluated both platforms in regard to coverage, reproducibility, and identification of differential methylation. We conclude that with current analytic goals microarrays still outperform bisulphite sequencing for precise quantification of DNA methylation.
- Published
- 2020
- Full Text
- View/download PDF
30. Short- and intermediate-term exposure to ambient fine particulate elements and leukocyte epigenome-wide DNA methylation in older men: the Normative Aging Study
- Author
-
Cuicui Wang, Andres Cardenas, John N. Hutchinson, Allan Just, Jonathan Heiss, Lifang Hou, Yinan Zheng, Brent A. Coull, Anna Kosheleva, Petros Koutrakis, Andrea A. Baccarelli, and Joel D. Schwartz
- Subjects
PM2.5 ,PM2.5 elements ,DNA methylation ,Epigenome-wide association study ,Distributed-lag ,Pathway analyses ,Environmental sciences ,GE1-350 - Abstract
Background: Several epigenome-wide association studies (EWAS) of ambient particulate matter with aerodynamic diameter ≤ 2.5 µm (PM2.5) have been reported. However, EWAS of PM2.5 elements (PEs), reflecting different emission sources, are very limited. Objectives: We performed EWAS of short- and intermediate-term exposure to PM2.5 and 13 PEs. We hypothesized that significant changes in DNAm may vary by PM2.5 mass and its elements. Methods: We repeatedly collected blood samples in the Normative Aging Study and measured leukocyte DNA methylation (DNAm) with the Illumina HumanMethylation450K BeadChip. We collected daily PM2.5 and 13 PEs at a fixed central site. To estimate the associations between each PE and DNAm at individual cytosine-phosphate-guanine (CpG) sites, we incorporated a distributed-lag (0–27 d) term in the setting of median regression with subject-specific intercept and examined cumulative lag associations. We also accounted for selection bias due to loss to follow-up and mortality prior to enrollment. Significantly differentially methylated probes (DMPs) were identified using Bonferroni correction for multiple testing. We further conducted regional and pathway analyses to identify significantly differentially methylated regions (DMRs) and pathways. Results: We included 695 men with 1,266 visits between 1999 and 2013. The subjects had a mean age of 75 years. The significant DMPs, DMRs, and pathways varied by to PM2.5 total mass and PEs. For example, PM2.5 total mass was associated with 2,717 DMPs and 10,470 DMRs whereas Pb was associated with 3,173 DMPs and 637 DMRs. The identified pathways by PM2.5 mass were mostly involved in mood disorders, neuroplasticity, immunity, and inflammation, whereas the pathways associated with motor vehicles (BC, Cu, Pb, and Zn) were related with cardiovascular disease and cancer (e.g., “PPARs signaling”). Conclusions: PM2.5 and PE were associated with methylation changes at multiple probes and along multiple pathways, in ways that varied by particle components.
- Published
- 2022
- Full Text
- View/download PDF
31. Improved filtering of DNA methylation microarray data by detection p values and its impact on downstream analyses
- Author
-
Jonathan A. Heiss and Allan C. Just
- Subjects
DNA methylation ,Microarray analysis ,Illumina 450K ,Outlier detection ,Data cleaning ,EWAS ,Medicine ,Genetics ,QH426-470 - Abstract
Abstract Background DNA methylation microarrays are popular for epigenome-wide association studies (EWAS), but spurious values complicate downstream analysis and threaten replication. Conventional cut-offs for detection p values for filtering out undetected probes were demonstrated in a single previous study as insufficient leading to many apparent methylation calls in samples from females in probes targeting the Y-chromosome. We present an alternative approach to calculate more accurate detection p values utilizing non-specific background fluorescence. We evaluate and compare our proposed approach of filtering observations with conventional ones by assessing the detection of Y-chromosome probes among males and females in 2755 samples from 17 studies on the 450K microarray and masking of large outliers between technical replicates and their impact downstream via an EWAS reanalysis. Results In contrast to conventional approaches, ours marks most Y-chromosome probes in females as undetected while removing a median of only 0.14% of the data per sample, catches more (30% vs. 6%) of large outliers (more than 20 percentage point difference between technical replicates), and helps to identify strong associations previously obfuscated by outliers between whole blood DNA methylation and chronological age in a well-powered EWAS (n = 729). Conclusions We provide guidance for filtering both 450K and EPIC microarrays as an essential preprocessing step to reduce spurious values. An implementation (including a function compatible with objects from the popular minfi package) was added to ewastools, an R package for comprehensive quality control of DNA methylation microarrays. Scripts to reproduce all analyses are available at doi.org/10.5281/zenodo.1443561.
- Published
- 2019
- Full Text
- View/download PDF
32. Analysis of repeated leukocyte DNA methylation assessments reveals persistent epigenetic alterations after an incident myocardial infarction
- Author
-
Cavin K. Ward-Caviness, Golareh Agha, Brian H. Chen, Liliane Pfeiffer, Rory Wilson, Petra Wolf, Christian Gieger, Joel Schwartz, Pantel S. Vokonas, Lifang Hou, Allan C. Just, Stefania Bandinelli, Dena G. Hernandez, Andrew B. Singleton, Holger Prokisch, Thomas Meitinger, Gabi Kastenmüller, Luigi Ferrucci, Andrea A. Baccarelli, Melanie Waldenberger, and Annette Peters
- Subjects
Myocardial infarction ,DNA methylation ,Epigenetics ,Fingerprint ,Epigenetic fingerprint ,Metabolites ,Medicine ,Genetics ,QH426-470 - Abstract
Abstract Background Most research into myocardial infarctions (MIs) have focused on preventative efforts. For survivors, the occurrence of an MI represents a major clinical event that can have long-lasting consequences. There has been little to no research into the molecular changes that can occur as a result of an incident MI. Here, we use three cohorts to identify epigenetic changes that are indicative of an incident MI and their association with gene expression and metabolomics. Results Using paired samples from the KORA cohort, we screened for DNA methylation loci (CpGs) whose change in methylation is potentially indicative of the occurrence of an incident MI between the baseline and follow-up exams. We used paired samples from the NAS cohort to identify 11 CpGs which were predictive in an independent cohort. After removing two CpGs associated with medication usage, we were left with an “epigenetic fingerprint” of MI composed of nine CpGs. We tested this fingerprint in the InCHIANTI cohort where it moderately discriminated incident MI occurrence (AUC = 0.61, P = 6.5 × 10−3). Returning to KORA, we associated the epigenetic fingerprint loci with cis-gene expression and integrated it into a gene expression-metabolomic network, which revealed links between the epigenetic fingerprint CpGs and branched chain amino acid (BCAA) metabolism. Conclusions There are significant changes in DNA methylation after an incident MI. Nine of these CpGs show consistent changes in multiple cohorts, significantly discriminate MI in independent cohorts, and were independent of medication usage. Integration with gene expression and metabolomics data indicates a link between MI-associated epigenetic changes and BCAA metabolism.
- Published
- 2018
- Full Text
- View/download PDF
33. Metastable DNA methylation sites associated with longitudinal lung function decline and aging in humans: an epigenome-wide study in the NAS and KORA cohorts
- Author
-
Juan Jose Carmona, Richard T. Barfield, Tommaso Panni, Jamaji C. Nwanaji-Enwerem, Allan C. Just, John N. Hutchinson, Elena Colicino, Stefan Karrasch, Simone Wahl, Sonja Kunze, Nadereh Jafari, Yinan Zheng, Lifang Hou, Dawn L. DeMeo, Augusto A. Litonjua, Pantel S. Vokonas, Annette Peters, Xihong Lin, Joel Schwartz, Holger Schulz, and Andrea A. Baccarelli
- Subjects
biomarker ,dna methylation ,pulmonary function ,lung function decline ,Genetics ,QH426-470 - Abstract
DNA methylation is an epigenetic regulator of gene transcription, which has been found to be both metastable and variable within human cohort studies. Currently, few studies have been done to identify metastable DNA methylation biomarkers associated with longitudinal lung function decline in humans. The identification of such biomarkers is important for screening vulnerable populations. We hypothesized that quantifiable blood-based DNA methylation alterations would serve as metastable biomarkers of lung function decline and aging, which may help to discover new pathways and/or mechanisms related to pulmonary pathogenesis. Using linear mixed models, we performed an Epigenome Wide Association Study (EWAS) between DNA methylation at CpG dinucleotides and longitudinal lung function (FVC, FEV1, FEF25–75%) decline and aging with initial discovery in the Normative Aging Study, and replication in the Cooperative Health Research in the Region of Augsburg cohort. We identified two metastable epigenetic loci associated with either poor lung function and aging, cg05575921 (AHRR gene), or lung function independently of aging, cg06126421 (IER3 gene). These loci may inform basic mechanisms associated with pulmonary function, pathogenesis, and aging. Human epigenomic variation, may help explain features of lung function decline and related pathophysiology not attributable to DNA sequence alone, such as accelerated pulmonary decline in smokers, former smokers, and perhaps non-smokers. Our EWAS across two cohorts, therefore, will likely have implications for the human population, not just the elderly.
- Published
- 2018
- Full Text
- View/download PDF
34. DNA methylation in blood as a mediator of the association of mid-childhood body mass index with cardio-metabolic risk score in early adolescence
- Author
-
Jian V. Huang, Andres Cardenas, Elena Colicino, C. Mary Schooling, Sheryl L. Rifas-Shiman, Golareh Agha, Yinan Zheng, Lifang Hou, Allan C. Just, Augusto A. Litonjua, Dawn L. DeMeo, Xihong Lin, Emily Oken, Marie-France Hivert, and Andrea A. Baccarelli
- Subjects
obesity ,bmi ,cardio-metabolic ,dna methylation ,epigenetics ,mediation ,Genetics ,QH426-470 - Abstract
Obesity is associated with higher cardio-metabolic risk even in childhood and adolescence; whether this association is mediated by epigenetic mechanisms remains unclear. We examined the extent to which mid-childhood body mass index (BMI) z-score (median age 7.7 years) was associated with cardio-metabolic risk score in early adolescence (median age 12.9 years) via mid-childhood DNA methylation among 265 children in the Project Viva. We measured DNA methylation in leukocytes using the Infinium Human Methylation450K BeadChip. We assessed mediation CpG-by-CpG using epigenome-wide association analyses, high-dimensional mediation analysis, and natural effect models. We observed mediation by mid-childhood DNA methylation at 6 CpGs for the association between mid-childhood BMI z-score and cardio-metabolic risk score in early adolescence in the high-dimensional mediation analysis (accounting for 10% of the total effect) and in the natural effect model (β = 0.04, P = 3.2e-2, accounting for 13% of the total effect). The natural direct effect of BMI z-score on cardio-metabolic risk score was still evident (β = 0.27, P = 1.1e-25). We also observed mediation by mid-childhood DNA methylation at 5 CpGs that was in the opposite direction from the total effect (natural effect model: β = −0.04, P = 2.0e-2). Mediation in different directions implies a complex role of DNA methylation in the association between BMI and cardio-metabolic risk and needs further investigation. Future studies with larger sample size and greater variability in cardio-metabolic risk will further help elucidate the role of DNA methylation for cardio-metabolic risk.
- Published
- 2018
- Full Text
- View/download PDF
35. Cumulative lifetime maternal stress and epigenome-wide placental DNA methylation in the PRISM cohort
- Author
-
Kelly J. Brunst, Nicole Tignor, Allan Just, Zhonghua Liu, Xihong Lin, Michele R. Hacker, Michelle Bosquet Enlow, Robert O. Wright, Pei Wang, Andrea A. Baccarelli, and Rosalind J. Wright
- Subjects
dna methylation ,maternal stress ,placenta ,prism cohort ,metabolism ,endocytosis ,Genetics ,QH426-470 - Abstract
Evolving evidence links maternal stress exposure to changes in placental DNA methylation of specific genes regulating placental function that may have implications for the programming of a host of chronic disorders. Few studies have implemented an epigenome-wide approach. Using the Infinium HumanMethylation450 BeadChip (450K), we investigated epigenome-wide placental DNA methylation in relation to maternal experiences of traumatic and non-traumatic stressors over her lifetime assessed using the Life Stressor Checklist-Revised (LSC-R) survey (n = 207). We found differential DNA methylation at epigenome-wide statistical significance (FDR = 0.05) for 112 CpGs. Additionally, we observed three clusters that exhibited differential methylation in response to high maternal lifetime stress. Enrichment analyses, conducted at an FDR = 0.20, revealed lysine degradation to be the most significant pathway associated with maternal lifetimes stress exposure. Targeted enrichment analyses of the three largest clusters of probes, identified using the gap statistic, were enriched for genes associated with endocytosis (i.e., SMAP1, ANKFY1), tight junctions (i.e., EPB41L4B), and metabolic pathways (i.e., INPP5E, EEF1B2). These pathways, also identified in the top 10 KEGG pathways associated with maternal lifetime stress exposure, play important roles in multiple physiological functions necessary for proper fetal development. Further, two genes were identified to exhibit multiple probes associated with maternal lifetime stress (i.e., ANKFY1, TM6SF1). The methylation status of the probes belonging to each cluster and/or genes exhibiting multiple hits, may play a role in the pathogenesis of adverse health outcomes in children born to mothers with increased lifetime stress exposure.
- Published
- 2018
- Full Text
- View/download PDF
36. Identifying mislabeled and contaminated DNA methylation microarray data: an extended quality control toolset with examples from GEO
- Author
-
Jonathan A. Heiss and Allan C. Just
- Subjects
DNA methylation ,Epigenomics ,Infinium ,450K ,EPIC ,Quality control ,Medicine ,Genetics ,QH426-470 - Abstract
Abstract Background Mislabeled, contaminated or poorly performing samples can threaten power in methylation microarray analyses or even result in spurious associations. We describe a set of quality checks for the popular Illumina 450K and EPIC microarrays to identify problematic samples and demonstrate their application in publicly available datasets. Methods Quality checks implemented here include 17 control metrics defined by the manufacturer, a sex check to detect mislabeled sex-discordant samples, and both an identity check for fingerprinting sample donors and a measure of sample contamination based on probes querying high-frequency SNPs. These checks were tested on 80 datasets comprising 8327 samples run on the 450K microarray from the GEO repository. Results Nine hundred forty samples were flagged by at least one control metric and 133 samples from 20 datasets were assigned the wrong sex. In a dataset in which a subset of samples appear contaminated with a single source of DNA, we demonstrate that our measure based on outliers among SNP probes was strongly correlated (> 0.95) with another independent measure of contamination. Conclusions A more complete examination of samples that may be mislabeled, contaminated, or have poor performance due to technical problems will improve downstream analyses and replication of findings. We demonstrate that quality control problems are prevalent in a public repository of DNA methylation data. We advocate for a more thorough quality control workflow in epigenome-wide association studies and provide a software package to perform the checks described in this work. Reproducible code and supplementary material are available at https://doi.org/10.5281/zenodo.1172730.
- Published
- 2018
- Full Text
- View/download PDF
37. Differential DNA methylation and PM2.5 species in a 450K epigenome-wide association study
- Author
-
Lingzhen Dai, Amar Mehta, Irina Mordukhovich, Allan C. Just, Jincheng Shen, Lifang Hou, Petros Koutrakis, David Sparrow, Pantel S. Vokonas, Andrea A. Baccarelli, and Joel D. Schwartz
- Subjects
ambient particulate matter ,dna methylation ,epigenome-wide association study ,metals ,pathway enrichment ,Genetics ,QH426-470 - Abstract
Although there is growing evidence that exposure to ambient particulate matter is associated with global DNA methylation and gene-specific methylation, little is known regarding epigenome-wide changes in DNA methylation in relation to particles and, especially, particle components. Using the Illumina Infinium HumanMethylation450 BeadChip, we examined the relationship between one-year moving averages of PM2.5 species (Al, Ca, Cu, Fe, K, Na, Ni, S, Si, V, and Zn) and DNA methylation at 484,613 CpG probes in a longitudinal cohort that included 646 subjects. Bonferroni correction was applied to adjust for multiple comparisons. Bioinformatics analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment was also performed. We observed 20 Bonferroni significant (P-value < 9.4× 10−9) CpGs for Fe, 8 for Ni, and 1 for V. Particularly, methylation at Schlafen Family Member 11 (SLFN11) cg10911913 was positively associated with measured levels of all 3 species. The SLFN11 gene codes for an interferon-induced protein that inhibits retroviruses and sensitizes cancer cells to DNA-damaging agents. Bioinformatics analysis suggests that gene targets may be relevant to pathways including cancers, signal transduction, and cell growth and death. Ours is the first study to examine the epigenome-wide association between ambient particles species and DNA methylation. We found that long-term exposures to specific components of ambient particle pollution, especially particles emitted during oil combustion, were associated with methylation changes in genes relevant to immune responses. Our findings provide insight into potential biologic mechanisms on an epigenetic level.
- Published
- 2017
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.