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1. Use of M13 single-stranded DNA digoxigenin labelled probe for detection of human parvovirus B19 viraemia.

Author

Prato C, Paper T, and Morinet F

Subjects
Base Sequence, Digoxigenin, Humans, Molecular Sequence Data, Sensitivity and Specificity, DNA Probes, Parvovirus B19, Human isolation & purification
Abstract

A dot-blot hybridization assay for serum B19 DNA is described using a non-radioactive (digoxigenin) M13 single-stranded DNA probe. The assay is sensitive (0.3 pg of B19 DNA) and has several advantages over similar assays which use radioactive or non-radioactive labelled plasmid probe for the routine detection of B19 DNA in sera.

Published
1991
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