1. Effect of acute nerve root compression on endoneurial fluid pressure and blood flow in rat dorsal root ganglia
- Author
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Shin-ichi Kikuchi, Tamaki Igarashi, Shoji Yabuki, and Robert R. Myers
- Subjects
Nerve root ,Ischemia ,Lumbar spinal canal stenosis ,Rats, Sprague-Dawley ,Sciatica ,Spinal Stenosis ,Dorsal root ganglion ,Ganglia, Spinal ,Edema ,Peripheral Nervous System ,Pressure ,medicine ,Animals ,Orthopedics and Sports Medicine ,business.industry ,Nerve Compression Syndromes ,Blood flow ,Anatomy ,medicine.disease ,Rats ,medicine.anatomical_structure ,Regional Blood Flow ,Anesthesia ,Acute Disease ,Crush injury ,Female ,medicine.symptom ,Spinal Nerve Roots ,business ,Low Back Pain ,Intervertebral Disc Displacement - Abstract
The objective of the current study was to test the hypothesis that crush injury to nerve root increases endoneurial fluid pressure (EFP) and decreases blood flow in the associated dorsal root ganglion (DRG). A total of 21 adult, female Sprague-Dawley rats had their left L5 nerve root and DRG exposed. The L5 nerve root was clamped for 2 s with a vascular suture clip just proximal to the DRG (compression group). Sham-operated animals without compression were used for control (control group). EFP was recorded with a servo-null micropipette system using a glass micropipette with tip diameter of 4 mum before and after 3 h of treatment. After the final measurement of EFP, DRG was excised and processed for histology. Blood flow in the DRG was continuously monitored by laser Doppler flow meter for 3 h. Three hours after treatment, EFP was 4.7+/-2.7 cm H(2)O in the compression group and 2.2+/-1.2 cm H(2)O in the control group (P
- Published
- 2005