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Your search keyword '"Shin-ichi Kikuchi"' showing total 3 results
Start Over Author "Shin-ichi Kikuchi" Topic dorsal root ganglion
3 results on '"Shin-ichi Kikuchi"'

1. Effect of acute nerve root compression on endoneurial fluid pressure and blood flow in rat dorsal root ganglia

Author

Shin-ichi Kikuchi, Tamaki Igarashi, Shoji Yabuki, and Robert R. Myers

Subjects
Nerve root, Ischemia, Lumbar spinal canal stenosis, Rats, Sprague-Dawley, Sciatica, Spinal Stenosis, Dorsal root ganglion, Ganglia, Spinal, Edema, Peripheral Nervous System, Pressure, medicine, Animals, Orthopedics and Sports Medicine, business.industry, Nerve Compression Syndromes, Blood flow, Anatomy, medicine.disease, Rats, medicine.anatomical_structure, Regional Blood Flow, Anesthesia, Acute Disease, Crush injury, Female, medicine.symptom, Spinal Nerve Roots, business, Low Back Pain, Intervertebral Disc Displacement
Abstract

The objective of the current study was to test the hypothesis that crush injury to nerve root increases endoneurial fluid pressure (EFP) and decreases blood flow in the associated dorsal root ganglion (DRG). A total of 21 adult, female Sprague-Dawley rats had their left L5 nerve root and DRG exposed. The L5 nerve root was clamped for 2 s with a vascular suture clip just proximal to the DRG (compression group). Sham-operated animals without compression were used for control (control group). EFP was recorded with a servo-null micropipette system using a glass micropipette with tip diameter of 4 mum before and after 3 h of treatment. After the final measurement of EFP, DRG was excised and processed for histology. Blood flow in the DRG was continuously monitored by laser Doppler flow meter for 3 h. Three hours after treatment, EFP was 4.7+/-2.7 cm H(2)O in the compression group and 2.2+/-1.2 cm H(2)O in the control group (P

Published
2005

2. The Effect of a 5-HT2A Receptor Antagonist on Pain-Related Behavior, Endogenous 5-Hydroxytryptamine Production, and the Expression 5-HT2A Receptors in Dorsal Root Ganglia in a Rat Lumbar Disc Herniation Model.

Author

Kinshi Kato, Miho Sekiguchi, Shin-ichi Kikuchi, and Shin-ichi Konno

Subjects
*SEROTONIN, *HERNIA, *NUCLEUS pulposus, *SPRAGUE Dawley rats, *ACETIC acid, *SCIATICA, *FREY'S syndrome
Abstract

Study Design. Controlled, interventional, animal study. Objective. To evaluate the effect of a 5-HT2A receptor antagonist on pain-related behavior, endogenous 5-hydroxytryptamine (5-HT) plasma levels, and expression of 5-HT2A receptors in dorsal root ganglia (DRGs) in a rat lumbar disc herniation model. Summary of Background Data. Application of nucleus pulposus on the nerve root induces immediate peripheral 5-HT production and the expression of 5-HT2A receptors in the adjacent DRG. However, the efficacy of a 5-HT2A receptor antagonist for pain relief in this situation and the mechanism remain unknown. Methods. Autologous nucleus pulposus was applied to the left L5 nerve root of 91 adult female Sprague-Dawley rats. The selective 5-HT2A receptor antagonist sarpogrelate hydrochloride (SPG; 1 mg/kg or 10 mg/kg) or vehicle was administered orally once a day from 1 to 21 days postoperatively. Von Frey tests were used to test pain behavior before and after surgery. To assess the effect of SPG on endogenous 5-HT release surrounding the inflamed nerve root, we measured levels of 5-hydroxyindole acetic acid, a 5-HT metabolite, in plasma. Expression of 5-HT2A receptors in the left L5 DRG was examined with immunoblotting. Results. The higher dose (10 mg/kg) of SPG significantly improved the mechanical withdrawal thresholds from 5 to 21 days after surgery compared with vehicle treatment. 5-hydroxyindole acetic acid in plasma was not significantly different among any groups at any time points. Both doses of SPG inhibited the expression of 5-HT2A receptors after surgery compared with vehicle treatment. Conclusion. A selective 5-HT2A receptor antagonist attenuated pain-related behavior and suppressed 5-HT2A receptor expression in the DRG, but did not affect peripheral 5-HT production. Selective 5-HT2A receptor antagonists may attenuate sciatica by blocking and downregulating 5-HT2A receptors in DRGs in lumbar disc herniation. [ABSTRACT FROM AUTHOR]

Published
2015
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3. Gene Expression Changes in Dorsal Root Ganglion of Rat Experimental Lumber Disc Herniation Models.

Author

Takayama, Bunji, Sekiguchi, Mihó, Yabuki, Shoji, Fujita, Isami, Shimada, Hideaki, and Shin-ichi Kikuchi

Subjects
*LUMBAR vertebrae, *HERNIA, *THORACIC vertebrae, *SPINAL surgery, *GENE expression
Abstract

The article attempts to clarify the mechanisms of painful radiculopathy caused by lumbar disc herniation (LDH) from the viewpoint of gene expression changes in dorsal root ganglion (DRG) of two rat models of LDH. It shows that mechanical and chemical factors caused altered gene expression in the DRG at seven days after surgery. The article suggests that the upregulation of IGF-1 might be a key factor in painful radiculopathy induced by mechanical factor.

Published
2008
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