27 results on '"Tao, Yuan-Xiang"'
Search Results
2. C/EBPβ Participates in Nerve Trauma-Induced TLR7 Upregulation in Primary Sensory Neurons
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He, Long, Cao, Jing, Jiang, Bao-Chun, Yang, Jian-Jun, Tao, Yuan-Xiang, and Ai, Yanqiu
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- 2022
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3. Effect of Pharmacological Inhibition of Fat-Mass and Obesity-Associated Protein on Nerve Trauma-Induced Pain Hypersensitivities
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Zheng, Bi-Xin, Guo, Xinying, Albik, Sfian, Eloy, Jean, and Tao, Yuan-Xiang
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- 2021
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4. RALY participates in nerve trauma‐induced nociceptive hypersensitivity through triggering Eif4g2 gene expression in primary sensory neurons.
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Huang, Lina, Sharma, Dilip, Feng, Xiaozhou, Pan, Zhiqiang, Wu, Shaogen, Munoz, Daisy, Bekker, Alex, Hu, Huijuan, and Tao, Yuan‐Xiang
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GENE expression ,SENSORY neurons ,DORSAL root ganglia ,PERIPHERAL nervous system ,NERVES ,RNA-binding proteins ,KOUNIS syndrome - Abstract
Background and Purpose: Peripheral nerve trauma‐induced dysregulation of pain‐associated genes in the primary sensory neurons of dorsal root ganglion (DRG) contributes to neuropathic pain genesis. RNA‐binding proteins participate in gene transcription. We hypothesized that RALY, an RNA‐binding protein, participated in nerve trauma‐induced dysregulation of DRG pain‐associated genes and nociceptive hypersensitivity. Methods and Results: Immunohistochemistry staining showed that RALY was expressed exclusively in the nuclei of DRG neurons. Peripheral nerve trauma caused by chronic constriction injury (CCI) of unilateral sciatic nerve produced time‐dependent increases in the levels of Raly mRNA and RALY protein in injured DRG. Blocking this increase through DRG microinjection of adeno‐associated virus 5 (AAV5)‐expressing Raly shRNA reduced the CCI‐induced elevation in the amount of eukaryotic initiation factor 4 gamma 2 (Eif4g2) mRNA and Eif4g2 protein in injured DRG and mitigated the development and maintenance of CCI‐induced nociceptive hypersensitivity, without altering basal (acute) response to noxious stimuli and locomotor activity. Mimicking DRG increased RALY through DRG microinjection of AAV5 expressing Raly mRNA up‐regulated the expression of Eif4g2 mRNA and Eif4g2 protein in the DRG and led to hypersensitive responses to noxious stimuli in the absence of nerve trauma. Mechanistically, CCI promoted the binding of RALY to the promoter of Eif4g2 gene and triggered its transcriptional activity. Conclusion and Implications: Our findings indicate that RALY participates in nerve trauma‐induced nociceptive hypersensitivity likely through transcriptionally triggering Eif4g2 expression in the DRG. RALY may be a potential target in neuropathic pain management. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Transcription factor EBF1 mitigates neuropathic pain by rescuing Kv1.2 expression in primary sensory neurons.
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Liang, Yingping, Sharma, Dilip, Wang, Bing, Wang, Huixing, Feng, Xiaozhou, Ma, Ruining, Berkman, Tolga, Char, Steven, Bekker, Alex, and Tao, Yuan-Xiang
- Abstract
Nerve injury-induced alternations of gene expression in primary sensory neurons of the dorsal root ganglion (DRG) are molecular basis of neuropathic pain genesis. Transcription factors regulate gene expression. In this study, we examined whether early B cell factor 1 (EBF1), a transcription factor, in the DRG, participated in neuropathic pain caused by chronic constriction injury (CCI) of the sciatic nerve. EBF1 was distributed exclusively in the neuronal nucleus and coexpressed with cytoplasmic/membrane Kv1.2 in individual DRG neurons. The expression of Ebf1 mRNA and protein was time-dependently downregulated in the ipsilateral lumbar (L) 3/4 DRGs after unilateral CCI. Rescuing this downregulation through microinjection of the adeno-associated virus 5 expressing full-length Ebf1 mRNA into the ipsilateral L3/4 DRGs reversed the CCI-induced decrease of DRG Kv1.2 expression and alleviated the development and maintenance of mechanical, heat and cold hypersensitivities. Conversely, mimicking the downregulation of DRG EBF1 through microinjection of AAV5-expressing Ebf1 shRNA into unilateral L3/4 DRGs produced a reduction of Kv1.2 expression in the ipsilateral L3/4 DRGs, spontaneous pain, and the enhanced responses to mechanical, heat and cold stimuli in naive mice. Mechanistically, EBF1 not only bound to the Kcna2 gene (encoding Kv1.2) promoter but also directly activated its activity. CCI decreased the EBF1 binding to the Kcna2 promoter in the ipsilateral L3/4 DRGs. Our findings suggest that DRG EBF1 downregulation contributes to neuropathic pain likely by losing its binding to Kcna2 promoter and subsequently silencing Kv1.2 expression in primary sensory neurons. Exogenous EBF1 administration may mitigate neuropathic pain by rescuing DRG Kv1.2 expression. [ABSTRACT FROM AUTHOR]
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- 2024
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6. TET1 Overexpression Mitigates Neuropathic Pain Through Rescuing the Expression of μ-Opioid Receptor and Kv1.2 in the Primary Sensory Neurons
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Wu, Qiang, Wei, Guihua, Ji, Fengtao, Jia, Shushan, Wu, Shaogen, Guo, Xinying, He, Long, Pan, Zhiqiang, Miao, Xuerong, Mao, Qingxiang, Yang, Yong, Cao, Minghui, and Tao, Yuan-Xiang
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- 2019
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7. A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression.
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Wang, Bing, Ma, Longfei, Guo, Xinying, Du, Shibin, Feng, Xiaozhou, Liang, Yingping, Govindarajalu, Gokulapriya, Wu, Shaogen, Liu, Tong, Li, Hong, Patel, Shivam, Bekker, Alex, Hu, Huijuan, and Tao, Yuan-Xiang
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LINCRNA ,GENE expression ,NEURALGIA ,CALCIUM-dependent potassium channels ,DORSAL root ganglia ,SCIATIC nerve injuries - Abstract
Nerve injury to peripheral somatosensory system causes refractory neuropathic pain. Maladaptive changes of gene expression in primary sensory neurons are considered molecular basis of this disorder. Long non-coding RNAs (lncRNAs) are key regulators of gene transcription; however, their significance in neuropathic pain remains largely elusive.Here, we reported a novel lncRNA, named sensory neuron-specific lncRNA (SS-lncRNA), for its expression exclusively in dorsal root ganglion (DRG) and trigeminal ganglion. SS-lncRNA was predominantly expressed in small DRG neurons and significantly downregulated due to a reduction of early B cell transcription factor 1 in injured DRG after nerve injury. Rescuing this downregulation reversed a decrease of the calcium-activated potassium channel subfamily N member 1 (KCNN1) in injured DRG and alleviated nerve injury-induced nociceptive hypersensitivity. Conversely, DRG downregulation of SS-lncRNA reduced the expression of KCNN1, decreased total potassium currents and afterhyperpolarization currents and increased excitability in DRG neurons and produced neuropathic pain symptoms.Mechanistically, downregulated SS-lncRNA resulted in the reductions of its binding to Kcnn1 promoter and heterogeneous nuclear ribonucleoprotein M (hnRNPM), consequent recruitment of less hnRNPM to the Kcnn1 promoter and silence of Kcnn1 gene transcription in injured DRG.These findings indicate that SS-lncRNA may relieve neuropathic pain through hnRNPM-mediated KCNN1 rescue in injured DRG and offer a novel therapeutic strategy specific for this disorder. [ABSTRACT FROM AUTHOR]
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- 2023
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8. Pain
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Chen, Jun, Han, Ji-Sheng, Zhao, Zhi-Qi, Wei, Feng, Hsieh, Jen-Chuen, Bao, Lan, Chen, Andrew CN, Dai, Yi, Fan, Bi-Fa, Gu, Jian-Guo, Hao, Shuang-Lin, Hu, San-Jue, Ji, Yong-Hua, Li, Yong-Jie, Li, Yun-Qing, Lin, Qing, Liu, Xian-Guo, Liu, Yan-Qing, Lu, Yan, Luo, Fei, Ma, Chao, Qiu, Yun-Hai, Rao, Zhi-Ren, Shi, Lin, Shyu, Bai-Chuang, Song, Xue-Jun, Tang, Jing-Shi, Tao, Yuan-Xiang, Wan, You, Wang, Jia-Shuang, Wang, Ke-Wei, Wang, Yun, Xu, Guang-Yin, Xu, Tian-Le, You, Hao-Jun, Yu, Long-Chuan, Yu, Sheng-Yuan, Zhang, Da-Ying, Zhang, De-Ren, Zhang, Jun-Ming, Zhang, Xu, Zhang, Yu-Qiu, Zhuo, Min, and Pfaff, Donald W., editor
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- 2013
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9. Peripheral nerve injury up-regulates expression of interactor protein for cytohesin exchange factor 1 (IPCEF1) mRNA in rat dorsal root ganglion
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Guan, Xiaowei, Zhu, Xuguang, and Tao, Yuan-Xiang
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- 2009
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10. Downregulation of a Dorsal Root Ganglion‐Specifically Enriched Long Noncoding RNA is Required for Neuropathic Pain by Negatively Regulating RALY‐Triggered Ehmt2 Expression.
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Pan, Zhiqiang, Du, Shibin, Wang, Kun, Guo, Xinying, Mao, Qingxiang, Feng, Xiaozhou, Huang, Lina, Wu, Shaogen, Hou, Bailing, Chang, Yun‐Juan, Liu, Tong, Chen, Tong, Li, Hong, Bachmann, Thomas, Bekker, Alex, Hu, Huijuan, and Tao, Yuan‐Xiang
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NEURALGIA ,LINCRNA ,OPIOID receptors ,RNA polymerase II ,DORSAL root ganglia ,DOWNREGULATION ,NERVE block - Abstract
Nerve injury‐induced maladaptive changes of gene expression in dorsal root ganglion (DRG) neurons contribute to neuropathic pain. Long non‐coding RNAs (lncRNAs) are emerging as key regulators of gene expression. Here, a conserved lncRNA is reported, named DRG‐specifically enriched lncRNA (DS‐lncRNA) for its high expression in DRG neurons. Peripheral nerve injury downregulates DS‐lncRNA in injured DRG due, in part, to silencing of POU domain, class 4, transcription factor 3, a transcription factor that interacts with the DS‐lncRNA gene promoter. Rescuing DS‐lncRNA downregulation blocks nerve injury‐induced increases in the transcriptional cofactor RALY‐triggered DRG Ehmt2 mRNA and its encoding G9a protein, reverses the G9a‐controlled downregulation of opioid receptors and Kcna2 in injured DRG, and attenuates nerve injury‐induced pain hypersensitivities in male mice. Conversely, DS‐lncRNA downregulation increases RALY‐triggered Ehmt2/G9a expression and correspondingly decreases opioid receptor and Kcna2 expression in DRG, leading to neuropathic pain symptoms in male mice in the absence of nerve injury. Mechanistically, downregulated DS‐lncRNA promotes more binding of increased RALY to RNA polymerase II and the Ehmt2 gene promoter and enhances Ehmt2 transcription in injured DRG. Thus, downregulation of DS‐lncRNA likely contributes to neuropathic pain by negatively regulating the expression of RALY‐triggered Ehmt2/G9a, a key neuropathic pain player, in DRG neurons. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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11. N6‐Methyladenosine Demethylase FTO Contributes to Neuropathic Pain by Stabilizing G9a Expression in Primary Sensory Neurons.
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Li, Yize, Guo, Xinying, Sun, Linlin, Xiao, Jifang, Su, Songxue, Du, Shibin, Li, Zhen, Wu, Shaogen, Liu, Weili, Mo, Kai, Xia, Shangzhou, Chang, Yun‐Juan, Denis, Daniel, and Tao, Yuan‐Xiang
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SENSORY neurons ,DORSAL root ganglia ,DEMETHYLASE ,PERIPHERAL nervous system ,RNA modification & restriction - Abstract
Nerve injury‐induced change in gene expression in primary sensory neurons of dorsal root ganglion (DRG) is critical for neuropathic pain genesis. N6‐methyladenosine (m6A) modification of RNA represents an additional layer of gene regulation. Here, it is reported that peripheral nerve injury increases the expression of the m6A demethylase fat‐mass and obesity‐associated proteins (FTO) in the injured DRG via the activation of Runx1, a transcription factor that binds to the Fto gene promoter. Mimicking this increase erases m6A in euchromatic histone lysine methyltransferase 2 (Ehmt2) mRNA (encoding the histone methyltransferase G9a) and elevates the level of G9a in DRG and leads to neuropathic pain symptoms. Conversely, blocking this increase reverses a loss of m6A sites in Ehmt2 mRNA and destabilizes the nerve injury‐induced G9a upregulation in the injured DRG and alleviates nerve injury‐associated pain hypersensitivities. FTO contributes to neuropathic pain likely through stabilizing nerve injury‐induced upregulation of G9a, a neuropathic pain initiator, in primary sensory neurons. [ABSTRACT FROM AUTHOR]
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- 2020
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12. DNMT3a‐triggered downregulation of K2p1.1 gene in primary sensory neurons contributes to paclitaxel‐induced neuropathic pain.
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Mao, Qingxiang, Wu, Shaogen, Gu, Xiyao, Du, Shibin, Mo, Kai, Sun, Linlin, Cao, Jing, Bekker, Alex, Chen, Liyong, and Tao, Yuan‐Xiang
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SENSORY neurons ,DORSAL root ganglia ,DOWNREGULATION ,DNA methyltransferases ,ANTINEOPLASTIC agents - Abstract
Antineoplastic drugs induce dramatic transcriptional changes in dorsal root ganglion (DRG) neurons, which may contribute to chemotherapy‐induced neuropathic pain. K2p1.1 controls neuronal excitability by setting the resting membrane potential. Here, we report that systemic injection of the chemotherapy agent paclitaxel time‐dependently downregulates the expression of K2p1.1 mRNA and its coding K2p1.1 protein in the DRG neurons. Rescuing this downregulation mitigates the development and maintenance of paclitaxel‐induced mechanical allodynia and heat hyperalgesia. Conversely, in the absence of paclitaxel administration, mimicking this downregulation decreases outward potassium current and increases excitability in the DRG neurons, leading to the enhanced responses to mechanical and heat stimuli. Mechanically, the downregulation of DRG K2p1.1 mRNA is attributed to paclitaxel‐induced increase in DRG DNMT3a, as blocking this increase reverses the paclitaxel‐induced the decrease of DRG K2p1.1 and mimicking this increase reduces DRG K2p1.1 expression. In addition, paclitaxel injection increases the binding of DNMT3a to the K2p1.1 gene promoter region and elevates the level of DNA methylation within this region in the DRG. These findings suggest that DNMT3a‐triggered downregulation of DRG K2p1.1 may contribute to chemotherapy‐induced neuropathic pain. What's new? Chemotherapy‐induced peripheral neuropathic pain (CIPNP) limits the dosage and selection of anti‐cancer drugs for patients and can lead to therapy discontinuation. While the mechanism underlying CIPNP remains unclear, two‐pore domain background potassium (K2p) channels, which influence neuronal activity, likely play a role. Here, in mice, systemic administration of paclitaxel was found to downregulate K2p1.1 in dorsal root ganglion (DRG) neurons. K2p1.1 knockdown, mimicking paclitaxel effects, resulted in reduced outward potassium channel current and increased DRG excitability. Elevated expression of the DNA methyltransferase DNMT3a contributed to DRG K2p1.1 downregulation, suggesting that both DNMT3a and DRG K2p1.1 are crucial to CIPNP development. [ABSTRACT FROM AUTHOR]
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- 2019
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13. Expression of acetyl-histone H3 and acetyl-histone H4 in dorsal root ganglion and spinal dorsal horn in rat chronic pain models.
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Liang, Lingli and Tao, Yuan-Xiang
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HISTONE acetylation , *CHRONIC pain , *DORSAL root ganglia , *HISTONE acetyltransferase , *IMMUNOFLUORESCENCE , *WESTERN immunoblotting - Abstract
Abstract Aims Histone acetylation and deacetylation are two histone posttranslational modifications that are usually controlled by histone acetyltransferases (HATs) and histone deacetylases (HDACs). Although HATs or HDACs Inhibitors could relieve pain hypersensitivities in chronic pain animal models, it is not clear on the expression of global histone acetylation in the dorsal root ganglion (DRG) or spinal dorsal horn in chronic pain conditions. Main methods A spinal nerve ligation (SNL)-induced neuropathic pain model and a complete Freund's adjuvant (CFA)-induced inflammatory pain model in rats were used to examine the expression of total acetyl-histone H3 (AcH3) and total acetyl-histone H4 (AcH4) by immunofluorescence or western blot. Key findings AcH3 and AcH4 not only localized in neuronal nuclei, but also in nuclei of glial cells in the DRG. Unilateral SNL induced the increase of AcH3 and AcH4 expression in the injured lumbar 5 (L5) DRG, but not in the uninjured L5 DRG or the spinal dorsal horn, while unilateral intraplantar injection of CFA increased AcH3 and AcH4 expression in the ipsilateral L4/5 spinal dorsal horn, but not in the L4/5 DRG. Significance These results provide morphological evidence for global histone acetylation expression in the DRG and spinal cord and indicate the differential expression in the DRG and spinal dorsal horn in different chronic pain models. More precise epigenetic mechanisms of histone acetylation on the target genes need to be revealed. [ABSTRACT FROM AUTHOR]
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- 2018
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14. Protein Kinase B/Akt Is Required for Complete Freund's Adjuvant-Induced Upregulation of Nav1.7 and Nav1.8 in Primary Sensory Neurons.
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Liang, Lingli, Fan, Longchang, Tao, Bo, Yaster, Myron, and Tao, Yuan-Xiang
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Abstract: Voltage-gated sodium channels (Nav) are essential for the generation and conduction of action potentials. Peripheral inflammation increases the expression of Nav1.7 and Nav1.8 in dorsal root ganglion (DRG) neurons, suggesting that they participate in the induction and maintenance of chronic inflammatory pain. However, how Nav1.7 and Nav1.8 are regulated in the DRG under inflammatory pain conditions remains unclear. Using a complete Freund's adjuvant (CFA)-induced chronic inflammatory pain model and Western blot analysis, we found that phosphorylated Akt (p-Akt) was significantly increased in the ipsilateral L4/5 DRGs of rats on days 3 and 7 after intraplantar CFA injection. Immunohistochemistry showed that the percentage of p-Akt-positive neurons in the DRG was also significantly increased in the ipsilateral L4/5 DRGs at these time points. Moreover, CFA injection increased the colocalization of p-Akt with Nav1.7 and Nav1.8 in L4/5 DRG neurons. Pretreatment of rats with an intrathecal injection of Akt inhibitor IV blocked CFA-induced thermal hyperalgesia and CFA-induced increases in Nav1.7 and Nav1.8 in the L4/5 DRGs on day 7 after CFA injection. Our findings suggest that the Akt pathway participates in inflammation-induced upregulation of Nav1.7 and Nav1.8 expression in DRG neurons. This participation might contribute to the maintenance of chronic inflammatory pain. Perspective: This article presents that inhibition of Akt blocks CFA-induced thermal hyperalgesia and CFA-induced increases in dorsal root ganglion Nav1.7 and Nav1.8. These findings have potential implications for use of Akt inhibitors to prevent and/or treat persistent inflammatory pain. [Copyright &y& Elsevier]
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- 2013
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15. Dynamic temporal and spatial regulation of mu opioid receptor expression in primary afferent neurons following spinal nerve injury
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Lee, Chun-Yi, Perez, Federico M., Wang, Wei, Guan, Xiaowei, Zhao, Xiuli, Fisher, Janet L., Guan, Yun, Sweitzer, Sarah M., Raja, Srinivasa N., and Tao, Yuan-Xiang
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SPATIAL variation ,OPIOID receptors ,GENE expression ,NEURONS ,SPINAL nerves ,NEUROPATHY ,PAIN management ,WESTERN immunoblotting ,WOUNDS & injuries - Abstract
Abstract: Despite using prescribed pain medications, patients with neuropathic pain continue to experience moderate to severe pain. There is a growing recognition of a potent peripheral opioid analgesia in models of inflammatory and neuropathic pain. The goal of this study was to characterize the temporal and spatial expression of mu opioid receptor (mOR) mRNA and protein in primary afferent neurons in a rat L5 spinal nerve ligation model of persistent neuropathic pain. Bilateral L4 and L5 dorsal root ganglia (DRGs), L4 and L5 spinal cord segments, and hind paw plantar skins were collected on days 0 (naïve), 3, 7, 14, and 35 post-spinal nerve ligation or post-sham surgery. We found that expression of mOR mRNA and protein in primary afferent neurons changed dynamically and site-specifically following L5 spinal nerve ligation. Real-time RT–PCR, immunohistochemistry, and Western blot analysis demonstrated a down-regulation of mOR mRNA and protein in the injured L5 DRG. In contrast, in the uninjured L4 DRG, mOR mRNA transiently decreased on day 7 and then increased significantly on day 14. Western blot analysis revealed a persistent increase in mOR protein expression, although immunohistochemistry showed no change in number of mOR-positive neurons in the uninjured L4 DRG. Interestingly, mOR protein expression was reduced in the skin on days 14 and 35 post-nerve injury and in the L4 and L5 spinal cord on day 35 post-nerve injury. These temporal and anatomically specific changes in mOR expression following nerve injury are likely to have functional consequences on pain-associated behaviors and opioid analgesia. [Copyright &y& Elsevier]
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- 2011
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16. Corrigendum: Role of MicroRNA-143 in Nerve Injury-Induced Upregulation of Dnmt3a Expression in Primary Sensory Neurons.
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Xu, Bo, Cao, Jing, Zhang, Jun, Jia, Shushan, Wu, Shaogen, Mo, Kai, Wei, Guihua, Liang, Lingli, Miao, Xuerong, Bekker, Alex, and Tao, Yuan-Xiang
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SENSORY neurons ,DORSAL root ganglia ,NERVES - Published
- 2020
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17. G9a inhibits CREB-triggered expression of mu opioid receptor in primary sensory neurons following peripheral nerve injury.
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Liang, Lingli, Zhao, Jian-Yuan, Gu, Xiyao, Wu, Shaogen, Mo, Kai, Xiong, Ming, Marie Lutz, Brianna, Bekker, Alex, and Tao, Yuan-Xiang
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CREB protein ,OPIOID receptors ,SENSORY neurons ,PERIPHERAL nerve injuries ,DORSAL root ganglia ,GENE expression - Abstract
Neuropathic pain, a distressing and debilitating disorder, is still poorly managed in clinic. Opioids, like morphine, remain the mainstay of prescribed medications in the treatment of this disorder, but their analgesic effects are highly unsatisfactory in part due to nerve injury-induced reduction of opioid receptors in the first-order sensory neurons of dorsal root ganglia. G9a is a repressor of gene expression. We found that nerve injury-induced increases in G9a and its catalyzed repressive marker H3K9m2 are responsible for epigenetic silencing of
Oprm1 ,Oprk1 , andOprd1 genes in the injured dorsal root ganglia. Blocking these increases rescued dorsal root gangliaOprm1 ,Oprk1 , andOprd1 gene expression and morphine or loperamide analgesia and prevented the development of morphine or loperamide-induced analgesic tolerance under neuropathic pain conditions. Conversely, mimicking these increases reduced the expression of three opioid receptors and promoted the mu opioid receptor-gated release of primary afferent neurotransmitters. Mechanistically, nerve injury-induced increases in the binding activity of G9a and H3K9me2 to theOprm1 gene were associated with the reduced binding of cyclic AMP response element binding protein to theOprm1 gene. These findings suggest that G9a participates in the nerve injury-induced reduction of theOprm1 gene likely through G9a-triggered blockage in the access of cyclic AMP response element binding protein to this gene. [ABSTRACT FROM AUTHOR]- Published
- 2016
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18. Dorsal root ganglion transcriptome analysis following peripheral nerve injury in mice.
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Wu, Shaogen, Marie Lutz, Brianna, Miao, Xuerong, Liang, Lingli, Mo, Kai, Chang, Yun-Juan, Du, Peicheng, Soteropoulos, Patricia, Tian, Bin, Kaufman, Andrew G., Bekker, Alex, Hu, Yali, and Tao, Yuan-Xiang
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DORSAL root ganglia ,NERVOUS system injuries ,RNA sequencing ,PAIN management ,LABORATORY mice - Abstract
Background: Peripheral nerve injury leads to changes in gene expression in primary sensory neurons of the injured dorsal root ganglia. These changes are believed to be involved in neuropathic pain genesis. Previously, these changes have been identified using gene microarrays or next generation RNA sequencing with poly-A tail selection, but these approaches cannot provide a more thorough analysis of gene expression alterations after nerve injury. Methods: The present study chose to eliminate mRNA poly-A tail selection and perform strand-specific next generation RNA sequencing to analyze whole transcriptomes in the injured dorsal root ganglia following spinal nerve ligation. Quantitative real-time reverse transcriptase polymerase chain reaction assay was carried out to verify the changes of some differentially expressed RNAs in the injured dorsal root ganglia after spinal nerve ligation. Results: Our results showed that more than 50 million (M) paired mapped sequences with strand information were yielded in each group (51.87 M–56.12 M in sham vs. 51.08 M–57.99 M in spinal nerve ligation). Six days after spinal nerve ligation, expression levels of 11,163 out of a total of 27,463 identified genes in the injured dorsal root ganglia significantly changed, of which 52.14% were upregulated and 47.86% downregulated. The largest transcriptional changes were observed in protein-coding genes (91.5%) followed by noncoding RNAs. Within 944 differentially expressed noncoding RNAs, the most significant changes were seen in long interspersed noncoding RNAs followed by antisense RNAs, processed transcripts, and pseudogenes. We observed a notable proportion of reads aligning to intronic regions in both groups (44.0% in sham vs. 49.6% in spinal nerve ligation). Using quantitative real-time polymerase chain reaction, we confirmed consistent differential expression of selected genes including Kcna2, Oprm1 as well as lncRNAs Gm21781 and 4732491K20Rik following spinal nerve ligation. Conclusion: Our findings suggest that next generation RNA sequencing can be used as a promising approach to analyze the changes of whole transcriptomes in dorsal root ganglia following nerve injury and to possibly identify new targets for prevention and treatment of neuropathic pain. [ABSTRACT FROM AUTHOR]
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- 2016
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19. Effect of intrathecal NIS-lncRNA antisense oligonucleotides on neuropathic pain caused by nerve trauma, chemotherapy, or diabetes mellitus.
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Wen, Chun-Hsien, Berkman, Tolga, Li, Xiang, Du, Shibin, Govindarajalu, Gokulapriya, Zhang, Haijun, Bekker, Alex, Davidson, Steve, and Tao, Yuan-Xiang
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SCIATIC nerve injuries , *NEURALGIA , *OLIGONUCLEOTIDES , *DORSAL root ganglia , *SMALL interfering RNA , *DIABETES - Abstract
Background: Blocking increased expression of nerve injury-specific long non-coding RNA (NIS-lncRNA) in injured dorsal root ganglia (DRG) through DRG microinjection of NIS-lncRNA small hairpin interfering RNA or generation of NIS-lncRNA knockdown mice mitigates neuropathic pain. However, these strategies are impractical in the clinic. This study employed a Food and Drug Administration (FDA)-approved antisense oligonucleotides strategy to examine the effect of NIS-lncRNA ASOs on neuropathic pain.Methods: Effects of intrathecal injection of NIS-lncRNA antisense oligonucleotides on day 7 or 14 after chronic constriction injury (CCI) of the sciatic nerve, fourth lumbar (L4) spinal nerve ligation, or intraperitoneal injection of paclitaxel or streptozotocin on the expression of DRG NIS-lncRNA and C-C chemokine ligand 2 (CCL2, an NIS-lncRNA downstream target) and nociceptive hypersensitivity were examined. We also assessed whether NIS-lncRNA antisense oligonucleotides produced cellular toxicity.Results: Intrathecal NIS-lncRNA antisense oligonucleotides attenuated CCI-induced mechanical allodynia, heat hyperalgesia, cold hyperalgesia, and ongoing nociceptive responses, without changing basal or acute nociceptive responses and locomotor function. Intrathecal NIS-lncRNA antisense oligonucleotides also blocked CCI-induced increases in NIS-lncRNA and CCL2 in the ipsilateral L3 and L4 DRG and hyperactivities of neurones and astrocytes in the ipsilateral L3 and L4 spinal cord dorsal horn. Similar results were found in antisense oligonucleotides-treated mice after spinal nerve ligation or intraperitoneal injection of paclitaxel or streptozotocin. Normal morphologic structure and no cell loss were observed in the DRG and spinal cord of antisense oligonucleotides-treated mice.Conclusion: These findings further validate the role of NIS-lncRNA in trauma-, chemotherapy-, or diabetes-induced neuropathic pain and demonstrate potential clinical application of NIS-lncRNA antisense oligonucleotides for neuropathic pain management. [ABSTRACT FROM AUTHOR]- Published
- 2023
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20. E74-like factor 1 contributes to nerve trauma-induced nociceptive hypersensitivity via transcriptionally activating matrix metalloprotein-9 in dorsal root ganglion neurons.
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Zhang, Luyao, Li, Xiang, Feng, Xiaozhou, Berkman, Tolga, Ma, Ruining, Du, Shibin, Wu, Shaogen, Huang, Congcong, Amponsah, Akwasi, Bekker, Alex, and Tao, Yuan-Xiang
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DORSAL root ganglia , *SPINAL nerves , *PERIPHERAL nervous system , *NEURONS , *NERVES , *ALLERGIES - Abstract
Abstract: Nerve trauma-induced alternations of gene expression in the neurons of dorsal root ganglion (DRG) participate in nerve trauma-caused nociceptive hypersensitivity. Transcription factors regulate gene expression. Whether the transcription factor E74-like factor 1 (ELF1in the DRG contributes to neuropathic pain is unknown. We report here that peripheral nerve trauma caused by chronic constriction injury (CCI) of unilateral sciatic nerve or unilateral fourth lumbar spinal nerve ligation led to the time-dependent increases in the levels of Elf1 mRNA and ELF1 protein in injured DRG, but not in spinal cord. Preventing this increase through DRG microinjection of adeno-associated virus 5-expressing Elf1 shRNA attenuated the CCI-induced upregulation of matrix metallopeptidase 9 (MMP9) in injured DRG and induction and maintenance of nociceptive hypersensitivities, without changing locomotor functions and basal responses to acute mechanical, heat and cold stimuli. Mimicking this increase through DRG microinjection of AAV5-expressing full-length Elf1 upregulated DRG MMP9 and produced enhanced responses to mechanical, heat and cold stimuli in naïve mice. Mechanistically, more ELF1 directly bond to and activated Mmp9 promoter in injured DRG neurons following CCI. Our data indicate that ELF1 participates in nerve trauma-caused nociceptive hypersensitivity likely through upregulating MMP9 in injured DRG. ELF1 may be a new target for management of neuropathic pain. [ABSTRACT FROM AUTHOR]- Published
- 2023
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21. Eukaryotic initiation factor 4 gamma 2 contributes to neuropathic pain through down-regulation of Kv1.2 and the mu opioid receptor in mouse primary sensory neurones.
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Zhang, Zhen, Zheng, Bixin, Du, Shibin, Han, Guang, Zhao, Hui, Wu, Shaogen, Jia, Shushan, Bachmann, Thomas, Bekker, Alex, and Tao, Yuan-Xiang
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OPIOID receptors , *NEURONS , *DORSAL root ganglia , *SPINAL nerves , *ADENO-associated virus - Abstract
Background: Nerve injury-induced changes in gene expression in the dorsal root ganglion (DRG) contribute to neuropathic pain genesis. Eukaryotic initiation factor 4 gamma 2 (eIF4G2) is a general repressor of cap-dependent mRNA translation. Whether DRG eIF4G2 participates in nerve injury-induced alternations in gene expression and nociceptive hypersensitivity is unknown.Methods: The expression and distribution of eIF4G2 mRNA and protein in mouse DRG after spinal nerve ligation (SNL) were assessed. Effects of eIF4G2 siRNA microinjected through a glass micropipette into the injured DRG on the SNL-induced DRG mu opioid receptor (MOR) and Kv1.2 downregulation and nociceptive hypersensitivity were examined. In addition, effects of DRG microinjection of adeno-associated virus 5-expressing eIF4G2 (AAV5-eIF4G2) on basal DRG MOR and Kv1.2 expression and nociceptive thresholds were analysed.Results: eIF4G2 protein co-expressed with Kv1.2 and MOR in DRG neurones. Levels of eIF4G2 mRNA (1.7 [0.24] to 2.3 [0.14]-fold of sham, P<0.01) and protein (1.6 [0.14] to 2.5 [0.22]-fold of sham, P<0.01) in injured DRG were time-dependently increased on days 3-14 after SNL. Blocking increased eIF4G2 through microinjection of eIF4G2 siRNA into the injured DRG attenuated SNL-induced downregulation of DRG MOR and Kv1.2 and development and maintenance of nociceptive hypersensitivities. DRG microinjection of AAV5-eIF4G2 reduced DRG MOR and Kv1.2 expression and elicited hypersensitivities to mechanical, heat and cold stimuli in naïve mice.Conclusions: eIF4G2 contributes to neuropathic pain through participation in downregulation of Kv1.2 and MOR in injured DRG and is a potential target for treatment of this disorder. [ABSTRACT FROM AUTHOR]- Published
- 2021
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- View/download PDF
22. Systemic administration of NIS-lncRNA antisense oligonucleotide alleviates neuropathic pain.
- Author
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Berkman, Tolga, Li, Xiang, Liang, Yingping, Korban, Anna, Bekker, Alex, and Tao, Yuan-Xiang
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NEURALGIA , *DORSAL root ganglia , *NEUROLOGICAL disorders , *SUBCUTANEOUS injections , *THERAPEUTICS , *LINCRNA , *TERIPARATIDE - Abstract
• The ASO is an FDA-approved strategy in the treatment of neurological diseases. • Intrathecal NIS-lncRNA ASO produced a long-lasting antinociceptive effect on neuropathic pain. • Systemic administration of NIS-lncRNA ASO led to a similar antinociceptive effect. The antisense oligonucleotide (ASO) is an FDA-approved strategy in the treatment of neurological diseases. We have shown the viability of using intrathecal ASO to suppress nerve injury-specific long noncoding RNA (NIS-lncRNA) in dorsal root ganglion (DRG), resulting in a stable and long-lasting antinociceptive effect on NP. This study examined whether systemic administration of NIS-lncRNA ASO relieved the chronic constriction injury (CCI)-induced nociceptive hypersensitivity. A single subcutaneous injection of NIS-lncRNA ASO at a dose of 1,000 µg was carried out 7 days after CCI or sham surgery in male mice. Behavioral tests were performed one day before surgery and at different days after surgery. DRG and spinal cord were finally collected for quantitative real-time RT-PCR and Western blot assays. NIS-lncRNA ASO significantly alleviated CCI-induced mechanical allodynia, heat hyperalgesia, and cold hyperalgesia starting on day 14 or 21 post-ASO injection and lasting for at least 7 days on the ipsilateral side. Additionally, CCI-induced spontaneous pain and ipsilateral dorsal horn neuronal and astrocyte hyperactivation were blocked on day 28 after NIS-lncRNA ASO injection. As predicted, the CCI-induced increases in the levels of NIS-lncRNA and its downstream target C-C motif chemokine ligand 2 in the ipsilateral lumbar 3 and 4 DRGs were attenuated on day 28 following NIS-lncRNA ASO injection. Our findings indicate that systemic administration of NIS-lncRNA ASO also produces a stable and long-lasting antinociceptive effect on neuropathic pain. NIS-lncRNA ASO may have potential clinical application in the treatment of this disorder. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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23. Contribution of dorsal root ganglion octamer transcription factor 1 to neuropathic pain after peripheral nerve injury.
- Author
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Yuan, Jingjing, Wen, Jing, Wu, Shaogen, Mao, Yuanyuan, Mo, Kai, Li, Zhisong, Su, Songxue, Gu, Hanwen, Ai, Yanqiu, Bekker, Alex, Zhang, Wei, and Tao, Yuan-Xiang
- Subjects
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DORSAL root ganglia , *TRANSCRIPTION factors , *PERIPHERAL nervous system , *WOUNDS & injuries , *SCIATIC nerve injuries , *SCIATIC nerve , *SPINAL cord injuries - Abstract
Neuropathic pain genesis is related to gene alterations in the dorsal root ganglion (DRG) after peripheral nerve injury. Transcription factors control gene expression. In this study, we investigated whether octamer transcription factor 1 (OCT1), a transcription factor, contributed to neuropathic pain caused by chronic constriction injury (CCI) of the sciatic nerve. Chronic constriction injury produced a time-dependent increase in the level of OCT1 protein in the ipsilateral L4/5 DRG, but not in the spinal cord. Blocking this increase through microinjection of OCT1 siRNA into the ipsilateral L4/5 DRG attenuated the initiation and maintenance of CCI-induced mechanical allodynia, heat hyperalgesia, and cold allodynia and improved morphine analgesia after CCI, without affecting basal responses to acute mechanical, heat, and cold stimuli as well as locomotor functions. Mimicking this increase through microinjection of recombinant adeno-associated virus 5 harboring full-length OCT1 into the unilateral L4/5 DRG led to marked mechanical allodynia, heat hyperalgesia, and cold allodynia in naive rats. Mechanistically, OCT1 participated in CCI-induced increases in Dnmt3a mRNA and its protein and DNMT3a-mediated decreases in Oprm1 and Kcna2 mRNAs and their proteins in the injured DRG. These findings indicate that OCT1 may participate in neuropathic pain at least in part by transcriptionally activating Dnmt3a and subsequently epigenetic silencing of Oprm1 and Kcan2 in the DRG. OCT1 may serve as a potential target for therapeutic treatments against neuropathic pain. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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24. TET1 overexpression attenuates paclitaxel-induced neuropathic pain through rescuing K2p1.1 expression in primary sensory neurons of male rats.
- Author
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Jia, Shushan, Wei, Guihua, Bono, Jamie, Pan, Zhiqiang, Zheng, Bixin, Wang, Bing, Adaralegbe, Adejuyigbe, Tenorio, Christopher, Bekker, Alex, and Tao, Yuan-Xiang
- Subjects
- *
PACLITAXEL , *NEURALGIA , *POTASSIUM channels , *SENSORY neurons , *DORSAL root ganglia , *DNA demethylation , *GENETIC overexpression - Abstract
Paclitaxel-induced downregulation of two-pore domain K+ channel 1.1 (K 2p 1.1) caused by increasing DNA methylation within its gene promoter in the dorsal root ganglion (DRG) contributes to neuropathic pain. Given that ten-eleven translocation methylcytosine dioxygenase 1 (TET1) promotes DNA demethylation and gene transcription, the present study investigated whether DRG overexpression of TET1 produces an antinociceptive effect on the paclitaxel-induced nociceptive hypersensitivity. TET1 was overexpressed in the DRG through unilateral microinjection of the herpes simplex virus expressing full-length Tet1 mRNA into the fourth and fifth lumbar DRGs of male rats. Behavioral tests were carried out to examine the effect of this overexpression on the paclitaxel-induced nociceptive hypersensitivity. Western blot analysis, chromatin immunoprecipitation assay and 5-hydroxymethylcytosine detection assay were performed to assess the levels of TET1/K 2p 1.1, 5-methylcytosine and 5-hydroxymethylcytosine, respectively. DRG overexpression of TET1 mitigated the paclitaxel-induced mechanical allodynia, heat hyperalgesia and cold hyperalgesia on the ipsilateral side during the development and maintenance periods. Locomotor function or basal (acute) responses to mechanical, heat or cold stimuli were not affected. Mechanistically, DRG overexpression of TET1 rescued the expression of K 2p 1.1 by blocking the paclitaxel-induced increase in the level of 5-methylcytosine and correspondingly reversing the paclitaxel-induced decreases in the amount of 5-hydroxymethylcytosine within the K 2p 1.1 promoter region in the microinjected DRGs of male rats. Our findings suggest that DRG overexpression of TET1 alleviated chemotherapy-induced neuropathic pain likely through rescuing DRG K 2p 1.1 expression. Our findings may provide a potential avenue for the management of this disorder. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
25. mTOR and its downstream pathway are activated in the dorsal root ganglion and spinal cord after peripheral inflammation, but not after nerve injury.
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Liang, Lingli, Tao, Bo, Fan, Longchang, Yaster, Myron, Zhang, Yi, and Tao, Yuan-Xiang
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MTOR protein , *NEURAL crest , *CHRONIC pain , *INFLAMMATION , *SPINAL cord diseases , *NERVOUS system injuries , *GENETIC translation , *PATHOLOGICAL physiology - Abstract
Abstract: Protein translation controlled through activation of mammalian target of rapamycin (mTOR) participates in many physiological and pathological processes. However, whether such activation is required for chronic pain is still unknown. Here, we examined activation of the mTOR signaling pathway during complete Freund's adjuvant (CFA)-induced chronic inflammatory pain and L5 spinal nerve ligation (SNL)-induced neuropathic pain in rats. Western blot analysis showed significantly increased levels of phosphorylated mTOR (p-mTOR) and phosphorylated p70S6 kinase 1 (p-S6K1, a downstream effector of mTOR) in the ipsilateral L4/5 spinal cord 2h, 1 day, 3 days, and 7 days after intraplantar CFA injection and in the ipsilateral L4/5 dorsal root ganglions (DRGs) 1 and 3 days after CFA injection. Immunohistochemistry also demonstrated increases in number of p-mTOR-labeled neurons in the ipsilateral L4/5 DRGs and in density of p-mTOR-labeled immunoreactivity in the ipsilateral L4/5 superficial dorsal horn 1 day after CFA injection. Moreover, intrathecal administration of rapamycin, a selective inhibitor of mTOR, significantly blocked CFA-induced mechanical allodynia and thermal hyperalgesia 1 day post-CFA injection. Interestingly, expression of neither p-mTOR nor p-S6K1 was markedly altered on days 3, 7, or 14 after L5 SNL in L5 spinal cord or DRG. These findings indicate that in DRG and spinal cord, mTOR and S6K1 are activated during chronic inflammatory pain, but not during neuropathic pain. Our results strongly suggest that mTOR and its downstream pathway contribute to the development of chronic inflammatory pain. [Copyright &y& Elsevier]
- Published
- 2013
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26. Expression and distribution of mTOR, p70S6K, 4E-BP1, and their phosphorylated counterparts in rat dorsal root ganglion and spinal cord dorsal horn
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Xu, Ji-Tain, Zhao, Xiuli, Yaster, Myron, and Tao, Yuan-Xiang
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GENE expression , *SPINAL cord , *RAPAMYCIN , *PHOSPHORYLATION , *BRAIN physiology , *PROTEIN kinases , *MICROGLIA , *SUBSTANCE P - Abstract
Abstract: Mammalian target of rapamycin (mTOR) controls protein translation and has an important role in the mechanism of pain hypersensitivity under persistent pain conditions. However, its expression and localization in pain-related regions of the nervous system is not completely understood. Here, we examined the expression and distribution of mTOR, eukaryotic initiation factor 4E-binding protein1/2 (4E-BP1/2), p70 ribosomal S6 protein kinase (p70S6K), and their phosphorylated (active) counterparts in two major pain-related regions, the dorsal root ganglion (DRG) and spinal cord dorsal horn. Reverse transcriptase-polymerase chain reaction showed that mTOR, 4E-BP1, and p70S6K mRNA are expressed in the DRG and dorsal horn. Western blot analysis further confirmed the existence of their protein products in these two regions, but expression of their phosphorylated counterparts was very low in dorsal horn and was not detected in the DRG. Immunohistochemistry revealed mTOR and p70S6K in the DRG neurons. Quantitative analysis showed that approximately 26.1% (± 3.2%) of DRG neurons were positive for mTOR and 19.1% (± 1.9%) were positive for p70S6K. Most of these neurons were small—less than 600µm2 in cross-sectional area—and some co-labeled with substance P or isolectin B4. Surprisingly, 4E-BP1 was observed only in the DRG satellite glial cells. In the dorsal horn, mTOR, p70S6K, and 4E-BP1 were detected in neurons, but not in astrocytes or microglia. They were distributed in the whole dorsal horn, especially in the superficial dorsal horn. Immunostaining for their phosphorylated counterparts was very low or undetectable in DRG and dorsal horn. Behavioral study showed that intrathecal mTOR inhibitor, rapamycin, did not affect acute nocicepetive transmission. The results indicate that although mTOR, p70S6K, and 4E-BP1 are highly expressed in the DRG and dorsal horn, their activate forms are very low in both regions under normal conditions. Our findings support the view that mTOR and its downstream effectors do not play a key role in acute pain. [Copyright &y& Elsevier]
- Published
- 2010
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27. Nerve trauma-caused downregulation of opioid receptors in primary afferent neurons: Molecular mechanisms and potential managements.
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Zheng, Bi-Xin, Malik, Ayma, Xiong, Ming, Bekker, Alex, and Tao, Yuan-Xiang
- Subjects
- *
OPIOID receptors , *AFFERENT pathways , *DORSAL root ganglia , *DOWNREGULATION , *PERIPHERAL nervous system - Abstract
Neuropathic pain is the most common clinical disorder destroying the quality of patient life and leading to a marked economic and social burden. Opioids are still last option for pharmacological treatment of this disorder, but their antinociceptive effects are limited in part due to the downregulation of opioid receptors in the primary afferent neurons after peripheral nerve trauma. How this downregulation occurs is not completely understood, but recent studies have demonstrated that peripheral nerve trauma drives the alterations in epigenetic modifications (including DNA methylation, histone methylation and mciroRNAs), expression of transcription factors, post-transcriptional modifications (e.g., RNA methylation) and protein translation initiation in the neurons of nerve trauma-related dorsal root ganglion (DRG) and that these alternations may be associated with nerve trauma-caused downregulation of DRG opioid receptors. This review presents how opioid receptors are downregulated in the DRG after peripheral nerve trauma, specifically focusing on distinct molecular mechanisms underlying transcriptional and translational processes. This review also discusses how this downregulation contributes to the induction and maintenance of neuropathic pain. A deeper understanding of these molecular mechanisms likely provides a novel avenue for prevention and/or treatment of neuropathic pain. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
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