9 results on '"Xinru Li"'
Search Results
2. Synergistically Enhanced Antimetastasis Effects by Honokiol-Loaded pH-Sensitive Polymer–Doxorubicin Conjugate Micelles
- Author
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Xinru Li, Chuhang Zhou, Yuanhang Zhou, Shidi Han, Yan Liu, Yang Zou, Yao Jin, Yanxia Zhou, Chuyu He, and Yunqiang Deng
- Subjects
Honokiol ,Materials science ,Polymers ,Mice, Nude ,macromolecular substances ,02 engineering and technology ,Micelle ,Lignans ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,In vivo ,Cell Line, Tumor ,polycyclic compounds ,medicine ,Animals ,Humans ,General Materials Science ,Doxorubicin ,Cytotoxicity ,Micelles ,Drug Carriers ,Cell growth ,organic chemicals ,Biphenyl Compounds ,technology, industry, and agriculture ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,In vitro ,carbohydrates (lipids) ,chemistry ,030220 oncology & carcinogenesis ,Biophysics ,0210 nano-technology ,Conjugate ,medicine.drug - Abstract
In an effort to prevent metastasis of breast tumor cells- at the same time of inhibiting tumor growth with less toxic side effects, honokiol (HNK) was encapsulated into pH-sensitive polymeric micelles based on the conjugate of poly(2-ethyl-2-oxazoline)-poly(d,l-lactide) (PEOz-PLA) with doxorubicin (DOX), denoted as PEOz-PLA-imi-DOX. PEOz-PLA-imi-DOX was successfully synthesized by connecting DOX to the hydrophobic end of PEOz-PLA via acid-cleavable benzoic imine linker. HNK-loaded conjugate micelles (HNK/PP-DOX-PM) with a size of 21 nm and homogeneous spherical shape exhibited high drug-loading capacity. PEOz-PLA-imi-DOX and HNK/PP-DOX-PM displayed faster release of DOX at pH 5.0 than at pH 7.4. As anticipated, PEOz-PLA-imi-DOX maintained cytotoxicity of DOX against MDA-MB-231 cells. The synergistically enhanced in vitro antitumor effect of HNK/PP-DOX-PM was confirmed by their synergetic inhibition of MDA-MB-231 cell growth. Furthermore, the efficient prevention of tumor metastasis by HNK/PP-DOX-PM was testified by in vitro anti-invasion, wound healing and antimigration assessment in MDA-MB-231 cells, and in vivo bioluminescence imaging in nude mice. The suppression of growth and metastasis of tumor cells by HNK/PP-DOX-PM was attributed to the synergistic effect of pH-triggered drug release and HNK-aroused inhibition of matrix metalloproteinases and epithelial-mesenchymal transition, respectively. In addition, HNK/PP-DOX-PM exhibited superior biosafety than physically encapsulated dual-drug micelles. Consequently, the fabricated HNK/PP-DOX-PM may have great potential for safe and effective suppression of tumor growth and metastasis.
- Published
- 2018
3. Preparation and evaluation of doxorubicin-luminespib co-loaded multivesicular liposomes.
- Author
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Yajie Liu, Yu Wang, Jiajia Li, Xiaoqing Xu, and Xinru Li
- Subjects
LIPOSOMES ,DOXORUBICIN ,IN vitro studies ,PHARMACODYNAMICS - Abstract
Copyright of Journal of Chinese Pharmaceutical Sciences is the property of Journal of Chinese Pharmaceutical Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
- Full Text
- View/download PDF
4. Preparation and Evaluation of Liposomes Co-Loaded with Doxorubicin, Phospholipase D Inhibitor 5-Fluoro-2-Indolyl Deschlorohalopemide (FIPI) and D-Alpha Tocopheryl Acid Succinate (α-TOS) for Anti-Metastasis
- Author
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Wei Xiaomeng, Wenxi Zhang, Maoyuan Song, Jiongxi Lei, Yuanyuan Zhang, Mengya Yin, Jiajia Li, Yajie Liu, Xinru Li, Jiaxing Wang, Guanghua Peng, and Guiling Li
- Subjects
Materials science ,Cell ,Excipient ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Metastasis ,In vivo ,lcsh:TA401-492 ,medicine ,General Materials Science ,Doxorubicin ,DOX ,Liposome ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,medicine.disease ,Metastatic breast cancer ,In vitro ,0104 chemical sciences ,medicine.anatomical_structure ,Liposomes ,FIPI ,Cancer research ,lcsh:Materials of engineering and construction. Mechanics of materials ,α-TOS ,0210 nano-technology ,Anti-metastasis ,medicine.drug - Abstract
Tumor metastasis has become a key obstacle to cancer treatment, which causes high mortality. Nowadays, it involves multiple complex pathways, and conventional treatments are not effective due to fewer targets. The aims of the present study were to construct a novel liposome delivery system co-loading a specific PLD inhibitor 5-fluoro-2-indolyldes-chlorohalopemide (FIPI) in combination with antitumor drug doxorubicin (DOX) and functional excipient D-alpha tocopheryl acid succinate (α-TOS) for anti-metastasis. In this study, the liposomes containing three components (DFT-Lip) with different physicochemical properties were successfully prepared by film dispersion method combined with pH-gradient method. Physicochemical parameters such as particles size, potential, encapsulation efficiency, stability, and release profiles were investigated. In vitro and in vivo anti-metastasis effectiveness against highly metastatic breast cancer MDA-MB-231 cell line was evaluated. The liposomes showed uniform particle size (approximately 119 nm), high drug encapsulation efficiency (> 90%), slow release characteristics and stability. In vitro anti-tumor cell metastasis study demonstrated DFT-Lip could greatly inhibit motility, migration and invasion of MDA-MB-231 cells compared to other liposomes, predicting a synergistic anti-tumor metastasis effect between FIPI with α-TOS in liposomes. In vivo anti-metastasis study showed that DFT-Lip prevented the initiation and the progression of metastasis of high metastatic breast cancer. These results suggested that the liposomes containing DOX, FIPI, and α-TOS might be a promising strategy for metastatic tumor therapy in clinics.
- Published
- 2018
5. Combination of doxorubicin with harmine-loaded liposomes exerting synergistic antitumor efficacy
- Author
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Wenxi Zhang, Maoyuan Song, Jiongxi Lei, Xinru Li, Yan Liu, Shuangchen Cong, and Guanghua Peng
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0301 basic medicine ,Cell Survival ,Drug Compounding ,Pharmaceutical Science ,Context (language use) ,macromolecular substances ,02 engineering and technology ,Pharmacology ,Drug synergism ,03 medical and health sciences ,chemistry.chemical_compound ,Harmine ,Drug Delivery Systems ,Targeted liposomes ,Drug Discovery ,polycyclic compounds ,medicine ,Humans ,Doxorubicin ,Particle Size ,Cell survival ,Liposome ,Co delivery ,Antibiotics, Antineoplastic ,Organic Chemistry ,technology, industry, and agriculture ,Drug Synergism ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,Antineoplastic Agents, Phytogenic ,030104 developmental biology ,chemistry ,Liposomes ,MCF-7 Cells ,0210 nano-technology ,medicine.drug - Abstract
Long-circulation (PEGLip), pH-sensitive (PEOzLip), and active targeted liposomes (PEG-TATLip)-loading doxorubicin (DOX) and harmine (HM) were prepared. Their physicochemical properties and antitumor effect were investigated.The aims of the present study were to evaluate synergistic antitumor efficacy.Liposomes were prepared by using thin-film dispersion, active drug-loading and target post-insertion method. Subsequently physiochemical properties including particle size distribution, zeta potential, entrapment efficiency (EE), drug-loading content and in-vitro release were determined. Besides, the in vitro cytotoxicity of free drugs and drug-loaded liposomes was explored by using a Sulforhodamine-B Staining assay and the combination index values (CI Value) were calculated. Finally, the cellular uptake experiments by MCF-7cells were carried out via flow cytometry.All liposomes enhanced the antitumor effect significantly compared to free drugs. Among liposomes, PEG-TATLip enhanced the antitumor effect significantly compared to others. DOX and HM had moderate synergism with CI Value 0.85 for free drugs, 0.81 for PEGLip, 0.72 for PEOzLip, and 0.84 for PEG-TATLip respectively when the weight ratio of two drugs was 1:2. Moreover, the similarity between DOX and HM such as physicochemical properties, in vitro release modes and in vitro uptake kinetics characteristics when they were in the same formulations proved it possible for them to be delivered together.Active targeting liposomes were the most effective delivery system as compared with pH-sensitive and long circulation liposomes. Additionally, DOX and HM could be co-delivered in liposomes and they could play moderate synergism effect in antitumor efficacy.
- Published
- 2017
6. Poly(2-ethyl-2-oxazoline)–Doxorubicin Conjugate-Based Dual Endosomal pH-Sensitive Micelles with Enhanced Antitumor Efficacy
- Author
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Yushu Li, Lei Zhao, Xinru Li, Chao Zhang, Jinwen Li, Yan Liu, Chengwei Li, Yanxia Zhou, Dishi Wang, and Yajie Gao
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Endosome ,Stereochemistry ,media_common.quotation_subject ,Biomedical Engineering ,Mice, Nude ,Pharmaceutical Science ,Antineoplastic Agents ,Bioengineering ,Endosomes ,macromolecular substances ,Micelle ,Mice ,Structure-Activity Relationship ,Polyamines ,polycyclic compounds ,medicine ,Animals ,Humans ,Doxorubicin ,Internalization ,Cytotoxicity ,Micelles ,media_common ,Pharmacology ,Chemistry ,organic chemicals ,Organic Chemistry ,technology, industry, and agriculture ,Biological Transport ,Hydrogen-Ion Concentration ,Xenograft Model Antitumor Assays ,carbohydrates (lipids) ,Drug Liberation ,Drug delivery ,MCF-7 Cells ,Biophysics ,Female ,Linker ,Biotechnology ,Conjugate ,medicine.drug - Abstract
Dual endosomal pH-sensitive micelles were designed and fabricated to deliver doxorubicin (DOX) for treating breast cancer based on a poly(2-ethyl-2-oxazoline) (PEOz)-DOX (PEOz-hyd-DOX) conjugate. PEOz-hyd-DOX was successfully synthesized by connecting DOX to PEOz via an acid cleavable hydrazone linker and self-assembled into nanosized micelles, which further physically encapsulated DOX. The conjugate and DOX-loaded conjugate micelles displayed faster release of DOX at pH 5.0 than at pH 7.4. This pH-dependent release behavior might assist the quick diffusion of DOX from acidic endosomes or lysosomes and the intracellular transfer into the nucleus after internalization, which was confirmed by confocal laser scanning microscopy images. As expected, PEOz-hyd-DOX conjugate and DOX-loaded conjugate micelles maintained cytotoxicity of DOX. In addition, the dual endosomal pH-sensitive micelles were found to substantially enhance antitumor efficacy and reduce side effects compared with free DOX. Therefore, PEOz-hyd-DOX conjugate-based micelles might be potential drug delivery vehicles of DOX for safe and effective breast cancer therapy.
- Published
- 2014
7. A folate modified pH sensitive targeted polymeric micelle alleviated systemic toxicity of doxorubicin (DOX) in multi-drug resistant tumor bearing mice
- Author
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Xueqing Wang, Xiaoyou Wang, Qiang Zhang, Xinru Li, Zhiqiang Lin, Dong Mei, Xiucong Yang, Dan Wang, Wei Gao, and Bing He
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Drug ,Time Factors ,Heart Diseases ,Polymers ,media_common.quotation_subject ,Chemistry, Pharmaceutical ,Cell ,Pharmaceutical Science ,Mice, Nude ,macromolecular substances ,Pharmacology ,Micelle ,Folic Acid ,In vivo ,polycyclic compounds ,medicine ,Animals ,Humans ,Technology, Pharmaceutical ,Doxorubicin ,Particle Size ,Cytotoxicity ,Micelles ,media_common ,Drug Carriers ,Mice, Inbred BALB C ,Antibiotics, Antineoplastic ,Dose-Response Relationship, Drug ,Chemistry ,technology, industry, and agriculture ,Lung Injury ,Hydrogen-Ion Concentration ,Xenograft Model Antitumor Assays ,In vitro ,Drug Resistance, Multiple ,Tumor Burden ,carbohydrates (lipids) ,medicine.anatomical_structure ,Drug Resistance, Neoplasm ,MCF-7 Cells ,Female ,Intracellular ,medicine.drug - Abstract
Purpose The purpose of this work was to demonstrate the advantages of a folate modified pH sensitive micelle system (HPPF) on reducing the systemic toxicity of antitumor drug doxorubicin (DOX) as well as increasing the antitumor efficacy on multi-drug resistant tumor. Methods The micelle HPPF was fabricated by PHIS–PEG and Fol–PEG–PLA using dialysis method. Multi-drug resistant human breast-cancer cell (MCF-7Adr) was used to test the therapeutic effect of DOX loaded HPPF micelles (HPPF/DOX). Nude mice bearing MCF-7Adr tumor was used to evaluate the systemic toxicity of HPPF/DOX. Results The micelle HPPF was successfully prepared with good size uniformity and pH sensitivity. The in vitro experiments showed that HPPF significantly increased the intracellular level and cytotoxicity of DOX. The in vivo experiments demonstrated that HPPF had largely reduced the mortality and body weight loss, improved the animal status and decreased damages on heart and lung tissues comparing to free DOX. Conclusions The HPPF/DOX could significantly increase the antitumor efficacy of DOX and largely alleviate the systemic side effects induced by high dose DOX in the treatment of multi-drug resistant tumor.
- Published
- 2014
8. pH-responsive polymeric micelles based on poly(2-ethyl-2-oxazoline)-poly(D,L-lactide) for tumor-targeting and controlled delivery of doxorubicin and P-glycoprotein inhibitor
- Author
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Xinru Li, Lei Zhao, Yanxia Zhou, Yajie Gao, Dishi Wang, Shujin Ma, Yan Liu, Chao Zhang, Jinwen Li, and Yong Zhao
- Subjects
Male ,Materials science ,Magnetic Resonance Spectroscopy ,Polymers ,Polyesters ,Biomedical Engineering ,Mice, Nude ,Antineoplastic Agents ,Biocompatible Materials ,Polyethylene glycol ,Biochemistry ,Micelle ,Polyethylene Glycols ,Biomaterials ,Dioxanes ,chemistry.chemical_compound ,Mice ,Drug Delivery Systems ,In vivo ,Cations ,medicine ,Animals ,Vitamin E ,Doxorubicin ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,Lactic Acid ,Cytotoxicity ,Molecular Biology ,Oxazoles ,Micelles ,Mice, Inbred BALB C ,technology, industry, and agriculture ,Cationic polymerization ,General Medicine ,Hydrogen-Ion Concentration ,Flow Cytometry ,Drug Resistance, Multiple ,chemistry ,Microscopy, Fluorescence ,Drug Resistance, Neoplasm ,Critical micelle concentration ,Drug delivery ,Biophysics ,Biotechnology ,medicine.drug - Abstract
The combination of a chemotherapeutic drug with a P-glycoprotein (P-gp) inhibitor has emerged as a promising strategy for treating multidrug resistance (MDR) cancer. To ensure that two drugs can be co-delivered to the tumor region and quickly released in tumor cells, tumor-targeted and pH-sensitive polymeric micelles were designed and prepared by combining cationic ring-opening polymerization of 2-ethyl-2-oxazoline (EOz) with anionic ring-opening polymerization of D,L-lactide (LA), and then encapsulating doxorubicin (DOX) and D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS1000) into the micelles self-assembled by poly(2-ethyl-2-oxazoline)-poly(D,L-lactide) (PEOz-PLA) and DSPE-PEG-folate. PEOz-PLA exhibited a low critical micelle concentration and negligible cytotoxicity. The micelles enabled the rapid release of DOX when pH decreased from 7.4 to 5.0. The targeting ability of the micelles was demonstrated by in vitro flow cytometry in KBv cells and in vivo real time near-infrared fluorescence imaging in KBv tumor-bearing nude mice. The efficiency of MDR reversion for the micelles was testified by enhancement of intracellular DOX accumulation and cytotoxicity. The efficient drug delivery by the micelles was attributed to synergistic effects of folate-mediated targeting, pH-triggered drug release and TPGS1000-aroused P-gp inhibition. Therefore, the designed multifunctional polymeric micelles may have significant promise for therapeutic application of MDR cancer.
- Published
- 2014
9. Preparation and characterization of dual pH-sensitive polymer-doxorubicin conjugate micelles.
- Author
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Yang Zou, Yao Jin, Yuanhang Zhou, Chuyu He, Yunqiang Deng, Shidi Han, Chuhang Zhou, Xinru Li, Yanxia Zhou, and Yan Liu
- Subjects
MICELLES ,DOXORUBICIN ,TUMOR growth ,CONJUGATED polymers ,NUCLEAR magnetic resonance - Abstract
Copyright of Journal of Chinese Pharmaceutical Sciences is the property of Journal of Chinese Pharmaceutical Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2018
- Full Text
- View/download PDF
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