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Pipe material and residual chlorine are key factors for the drinking water distribution system, and understanding the biofilm ecosystem is vital for water quality safeguard. The aim of our study was to determine the influence of pipe materials (ductile iron, steel, polyethylene) and chlorination on the biofilm structure and microbial community, as shown by the physicochemical properties, extracellular polymeric substances (EPS) structural characteristics, bacterial community composition, and functional traits. EPS spatial properties were studied based on a semi-quantitative confocal laser scanning microscope (CLSM) description. Regarding the impact of chlorination, residule chlorine (1.0 ± 0.3 mg L -1 free chlorine) could inhibit the bacteria colonization, and initiate a potential response to external disinfectants revealed by the EPS spatial distribution changes and communities variation compared to unchlorinated system. Regarding the impact of pipe material, polyethylene (PE) biofilms displayed lower biomass, loose zoogloea structure, lower proteins and polysaccharides content, and poor microbial diversity in contrast to ductile iron and steel biofilms. Pipe material was the more possible driving factor of the biofilm community composition compared to the chlorination based on principal coordinates analysis (PCoA) and permutational multivariate analysis of variance (PERMANOVA). Actinobacteria was dominant in the PE biofilms (45.57%-83.32%), while Alphaproteobacteria (34.30%-73.22%) and Gammaproteobacteria (6.46%-36.82%) were the major classes in the steel and ductile iron biofilms. The genus Rhodococcus was predominant in the PE biofilms. Rhodococcus, Pseudomonas, and Sphingomonas seemed to have a better growth advantage in the chlorinated system and display a stronger disinfectant resistance. Functional sketch prediction indicated the potential impact of pipe material and chlorination on functional pathway abundnce, possible functional pathways associated with infectious disease included. This study provides insights into the impact of pipe material and chlorination on biofilm structure and microbial community and might help to develop monitoring or maintenance strategies to protect the biosafety of the drinking water., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

2,5-Dimethyl-p-phenylenediamine-1,3,5-triformylphloroglucinol covalent organic frameworks (PATP COF) were prepared and used as novel adsorbent for controlling the formation potential (FP) and reducing the toxic potential of both carbonaceous disinfection by-products (C-DBPs) and nitrogenous DBPs (N-DBPs) during their subsequent chlorination. During the PATP COF adsorption pretreatment process, the FP of C-DBPs, N-DBPs and total organic halogen (TOX) were reduced by 86.5, 75.4 and 81.1%, respectively. These removal efficiencies were significantly higher when compared with those obtained using a traditional activated carbon (AC) adsorption pretreatment process (42.7, 19.4 and 28.7%, respectively). By comprehensive toxicity calculations, a significant reduction in both the acute and chronic toxic potential of C-DBPs and N-DBPs were observed during the PATP COF adsorption process (with reduction rates of ~85 and ~ 75% observed for the C-DBPs and N-DBPs, respectively), which were comparable to the removal efficiencies observed for C-DBPs FP and N-DBPs FP by weight, suggesting the simultaneous and effective control of DBPs FP and their toxic potential. Cycling tests and stability trial also showed the excellent reusability, wide pH adaptability, and high stability of PATP COF, demonstrating its great potential application to the treatment of drinking water., Competing Interests: Declaration of competing interest The authors have declared that no conflict of interest exists., (Copyright © 2020 Elsevier B.V. All rights reserved.)

The emergence of nitrogenous disinfection byproducts (N-DBPs) in drinking water has become a widespread concern. In this study, dichloroacetonitrile (DCAN), dicholoacetamide (DCAcAm) and trichloronitromethane (TCNM) were chosen as representatives to clarify the characteristics of N-DBP precursors in the raw waters of Taihu Lake, the Yangtze River, and Gaoyou Lake. Removal of DCAN and DCAcAm precursors must focus on nonpolar and positively charged organics, but more attention should be paid to micromolecular, polar and non-positively charged organics as TCNM precursors. Compared to molecular weight (MW) and hydrophilicity fractionation, polarity and electrical classification have higher selectivity to intercept N-DBP precursors. The properties of N-DBP precursors are relatively fixed and traceable in water systems, which could contribute to their targeted removal. Based on investigation of their characteristics, the removal efficiency and preferences of organic precursors under different processes were studied in three drinking water treatment plants (DWTPs). The TCNM precursors produced in preozonation can be effectively removed during coagulation. The cumulative removal efficiency of conventional processes on N-DBP precursors was approximately 20-30%, but O 3 /BAC process improved removal by about 40%. The key to improving the removal efficiency of N-DBP precursors by O 3 /BAC is that it can significantly remove low-MW, nonpolar, positively charged, hydrophilic and transphilic organics. In combined toxicity trials, both cytotoxicity and genotoxicity showed a synergistic effect when DCAN, DCAcAm, and TCNM coexisted, which means that low-level toxicity enhancement in the actual water merits attention. DCAN precursors dominated in the toxicity formation potential (TFP), followed by TCNM precursors. In addition, the removal rate of total N-DBP precursors may be higher than that of TFP, leading to overly optimistic evaluation of precursor removal in water treatment practice. Therefore, the removal effect on TFP must also be considered., Competing Interests: Declaration of competing interest We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted., (Copyright © 2020 Elsevier B.V. All rights reserved.)