1. Design of dye and superparamagnetic iron oxide nanoparticle loaded lipid nanocapsules with dual detectability in vitro and in vivo.
- Author
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Bohley MS, Birch E, Baumann FJ, Dillinger AE, Tamm ER, and Goepferich AM
- Subjects
- Animals, Drug Carriers administration & dosage, Drug Carriers chemistry, Drug Compounding, Drug Liberation, Drug Stability, Endothelial Cells drug effects, Female, Fluorescent Dyes administration & dosage, Fluorescent Dyes chemistry, Humans, Mice, Mice, 129 Strain, Microscopy, Energy-Filtering Transmission Electron, Microscopy, Fluorescence, Nanocapsules chemistry, Particle Size, Chemistry, Pharmaceutical methods, Drug Carriers pharmacokinetics, Fluorescent Dyes pharmacokinetics, Lipids chemistry, Magnetic Iron Oxide Nanoparticles chemistry
- Abstract
Lipid nanocapsules are treasured nanoparticulate systems, although they lack detectability in biological environments. To overcome this, we designed LNCs loaded simultaneously with fluorescent dye and superparamagnetic iron oxide nanoparticles (Dual LNCs). The introduction of both labels did not alter nanoparticle characteristics such as size (50 nm), size distribution (polydispersity index < 0.1) or surface modifications, including the effectiveness of targeting ligands. Furthermore, the colloidal stability, particle integrity and biocompatibility of the nanoparticles were not negatively affected by label incorporation. These Dual LNCs are concomitantly visualizable via fluorescence and transmitted light imaging after either the internalization by cells or systemic administration to mice. Importantly, they are detectable in liver sections of mice using transmission electron microscopy without additional enhancement. The iron content of 0.24% (m/m) is sufficiently high for precise quantification of nanoparticle concentrations via inductively coupled plasma optical emission spectroscopy. Dual LNCs are precious tools for the investigation of in vitro and in vivo performances of lipid nanocapsule formulations, since they allow for the use of complementary imaging methods for broad range detectability., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
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