6 results on '"Wang, Yun F."'
Search Results
2. The lab's increasing arsenal of tools to battle antibiotic resistance.
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Wang, Yun F (Wayne)
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ANTIBIOTICS , *MASS spectrometry methodology , *DRUG resistance in microorganisms , *PATHOLOGICAL laboratories , *SOCIAL role , *DECISION making in clinical medicine , *TURNAROUND time - Abstract
The article offers information on the tools and technology being used in clinical microbiology laboratory to fight multidrug-resistant organisms (MDROs). It says that Grady Health System in Atlanta, Georgia acquired a so-called cutting-edge tool that can give data on pathogen identification and antimicrobial susceptibility (AMS). It says that the Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectometry (MALDI-TOF MS) helps bypass time-consuming tasks in culturing pathogen.
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- 2011
3. Time to Sputum Culture Conversion and Treatment Outcomes Among Patients with Isoniazid-Resistant Tuberculosis in Atlanta, Georgia.
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Schechter, Marcos C., Bizune, Destani, Kagei, Michelle, Machaidze, Mamuka, Holland, David P., Oladele, Alawode, Wang, Yun F., Rebolledo, Paulina A., Ray, Susan M., and Kempker, Russell R.
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DRUG therapy for tuberculosis , *FLUOROQUINOLONES , *CONFIDENCE intervals , *DRUG resistance in microorganisms , *ISONIAZID , *LONGITUDINAL method , *MICROBIAL sensitivity tests , *MULTIVARIATE analysis , *PROBABILITY theory , *SPUTUM , *STATISTICS , *TREATMENT effectiveness , *PROPORTIONAL hazards models , *RETROSPECTIVE studies , *DESCRIPTIVE statistics , *ODDS ratio , *THERAPEUTICS - Abstract
Background. Although isoniazid-resistant tuberculosis is more common than multidrug-resistant tuberculosis, it has been much less studied. We examined the impact of isoniazid resistance and treatment regimen, including use of a fluoroquinolone, on clinical outcomes. Methods. A retrospective cohort study among patients with sputum culture-positive tuberculosis was performed. Early fluoroquinolone (FQ) use was defined as receiving ≥5 doses during the first month of treatment. The primary outcome was time to sputum culture conversion (tSCC). A multivariate proportional hazards model was used to determine the association of isoniazid resistance with tSCC. Results. Among 236 patients with pulmonary tuberculosis, 59 (25%) had isoniazid resistance. The median tSCC was similar for isoniazid-resistant and -susceptible cases (35 vs 29 days; P = .39), and isoniazid resistance was not associated with tSCC in multivariate analysis (adjusted hazard ratio = 0.83; 95% confidence interval [CI], .59-1.17). Early FQ use was higher in isoniazid-resistant than -susceptible cases (20% vs 10%; P = .05); however, it was not significantly associated with tSCC in univariate analysis (hazard ratio = 1.48; 95% CI, .95-2.28). Patients with isoniazid-resistant tuberculosis were treated with regimens containing rifampin, pyrazinamide, and ethambutol +/- a FQ for a median of 9.7 months. Overall, 191 (83%) patients were cured. There was no difference in initial treatment outcomes; however, all cases of acquired-drug resistance (n = 1) and recurrence (n = 3) occurred among patients with isoniazid-resistant tuberculosis. Conclusions. There was no significant association with isoniazid resistance and tSCC or initial treatment outcomes. Although patients with isoniazid-resistant tuberculosis had a high cure rate, the cases of recurrence and acquired drug resistance are concerning and highlight the need for longer-term follow-up studies. [ABSTRACT FROM AUTHOR]
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- 2017
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4. Induction of human plasmablasts during infection with antibiotic-resistant nosocomial bacteria.
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Band, Victor I., Ibegbu, Chris, Kaur, Surinder Pal, Cagle, Stephanie M., Trible, Ronald, Jones, Crystal L., Wang, Yun F., Kraft, Colleen S., Ray, Susan M., Wrammert, Jens, and Weiss, David S.
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PATHOGENIC microorganisms , *NOSOCOMIAL infections , *B cells , *ANTIBIOTICS , *DRUG resistance in microorganisms - Abstract
Objectives Nosocomial pathogens such as Acinetobacter baumannii are a growing public health threat, due in part to their increasing resistance to antibiotics. Since some strains are resistant to all available antibiotics, novel therapies are urgently needed. Plasmablasts are short-lived B cells found in the blood that can be collected and harnessed to produce therapeutic antibodies. We set out to determine whether plasmablasts are induced during infection with A. baumannii and other nosocomial pathogens. Methods We obtained blood samples from patients infected with antibiotic-resistant nosocomial pathogens, and analysed their plasmablast response by flow cytometry. Results We observed a strong induction of plasmablasts in patients with antibiotic-resistant A. baumannii infection. Furthermore, plasmablasts were also induced in response to other drug-resistant nosocomial pathogens. Conclusions These data suggest that plasmablasts may be broadly harnessed to develop therapeutic antibodies to combat otherwise untreatable antibiotic-resistant infections. [ABSTRACT FROM PUBLISHER]
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- 2014
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5. Emergence of Community-Associated Methicillin-Resistant Staphylococcus aureus USA300 Genotype as a Major Cause of Health Care--Associated Blood Stream Infections.
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Seybold, Ulrich, Kourbatova, Ekaterina V., Johnson, James G., Halvosa, Sue J., Wang, Yun F., King, Mark D., Ray, Susan M., and Blumberg, Henry M.
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STAPHYLOCOCCUS aureus , *METHICILLIN resistance , *DRUG resistance in microorganisms , *EFFECT of drugs on microorganisms , *GENOTYPE-environment interaction , *BACTERIAL diseases - Abstract
Background. Whether community-associated methicillin-resistant Staphylococcus aureus (MRSA) genotypes (e.g., USA300) are a major cause of bloodstream infections (BSIs) and health care-associated infections has been poorly defined. Methods. Consecutive MRSA isolates recovered from patients with BSIs were prospectively collected at an urban public hospital. Molecular typing studies were performed. Prevalence and risk factors for the MRSA USA300 genotype were assessed. Results. One hundred thirty-two cases of MRSA BSI were documented over 7.5 months in 2004 (incidence, 6.79 per 1000 admissions); 116 isolates were available for genotyping. Characteristics of the 116 evaluable cases included: a mean age 47 years; 62% were male, 82% were African American, and 22% were HIV seropositive. The crude in-hospital mortality rate was 22%. In 107 cases (92%), there was contact with a health care facility within the year prior to infection, and a nosocomial infection (defined as positive blood culture results obtained >48 h after admission) occurred in 49 cases (42%). PFGE demonstrated that 39 (34%) of the 116 isolates were the MRSA USA300 genotype; 34 (29%) were USA100; 42 (36%) were USA500; and 1 (1%) was USA800. MRSA USA300 accounted for 28% of health care-associated BSIs and 20% of nosocomial MRSA BSIs. In multivariate analysis, isolation of the USA300 genotype was associated with injectiondrug use (OR, 3.67; 95% CI, 1.10–12.28) and skin and soft tissue infection (OR, 4.26; 95% CI, 1.08–16.84). Patients who resided in long-term care facilities (OR, 0.09; 95% CI, 0.01–0.82) and those who were treated with antimicrobials in the prior year were less likely to have MRSA USA300 genotype recovered (OR, 0.10; 95% CI, 0.02–0.49). Conclusions. MRSA USA300 genotype, the predominant cause of community-associated MRSA infections in our area (Atlanta, GA), has now emerged as a significant cause of health care-associated and nosocomial BSI. MRSA USA300 as a nosocomial pathogen presents new challenges to infection control programs. [ABSTRACT FROM AUTHOR]
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- 2006
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6. Discordance in Mycobacterium tuberculosis Rifampin Susceptibility.
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Kalokhe, Ameeta S., Shafiq, Majid, Lee, James C., Metchock, Beverly, Posey, James E., Ray, Susan M., Anderson, Albert, Wang, Yun F., and Nguyen, Minh Ly T.
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HOMELESS women , *AIDS patients , *TUBERCULOSIS , *DRUG resistance in microorganisms , *ISONIAZID , *RIFAMPIN , *ANTITUBERCULAR agents , *SYMPTOMS , *MYCOBACTERIUM tuberculosis , *AMINOGLYCOSIDES - Abstract
The article discusses the case of a 45-year-old homeless woman with acquired immune deficiency syndrome (AIDS) who experienced pulmonary tuberculosis (TB) relapse with an associated resistance to antituberculous drugs isoniazid (INH) and rifampin (RIF). Her symptoms included fever, night sweats, weight loss and cough. She tested positive for Mycobacterium tuberculosis complex in August 2009, while the culture of the sputum specimen obtained at her admission indicated that the isolate was resistant to INH but susceptible to RIF. After a week, mycobacteriology laboratory findings showed that the isolate was resistant to both INH and RIF. The patient underwent injectable aminoglycoside therapy following her readmission.
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- 2012
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