1. Simultaneous determination of vancomycin and ceftazidime in cerebrospinal fluid in craniotomy patients by high-performance liquid chromatography
- Author
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Biao Wang, Jierong Dong, Zhongxian Zhou, Xiaohua Yu, Guangming Ye, Yunyun Jiang, Jiandong Zhang, Weibin Wang, Yu-hai Wang, and Xue-jian Cai
- Subjects
Bioanalysis ,Acetonitriles ,Time Factors ,Clinical Biochemistry ,Pharmaceutical Science ,Ceftazidime ,Guidelines as Topic ,Microbial Sensitivity Tests ,Acetates ,Buffers ,High-performance liquid chromatography ,Sensitivity and Specificity ,Tinidazole ,Analytical Chemistry ,Drug Stability ,Vancomycin ,Spectrophotometry ,Drug Discovery ,Freezing ,medicine ,Protein precipitation ,Humans ,Spectroscopy ,Chromatography, High Pressure Liquid ,Antibacterial agent ,Chromatography ,medicine.diagnostic_test ,Molecular Structure ,Chemistry ,Temperature ,Reproducibility of Results ,Repeatability ,Hydrogen-Ion Concentration ,Anti-Bacterial Agents ,Spectrophotometry, Ultraviolet ,Craniotomy ,medicine.drug - Abstract
A simple, accurate and rapid method for simultaneous analysis of vancomycin and ceftazidime in cerebrospinal fluid (CSF), utilizing high-performance liquid chromatography (HPLC), has been developed and thoroughly validated to satisfy strict FDA guidelines for bioanalytical methods. Protein precipitation was used as the sample pretreatment method. In order to increase the accuracy, tinidazole was chosen as the internal standard. Separation was achieved on a Diamonsil C18 column (200 mm x 4.6mm I.D., 5 microm) using a mobile phase composed of acetonitrile and acetate buffer (pH 3.5) (8:92, v/v) at room temperature (25 degrees C), and the detection wavelength was 240 nm. All the validation data, such as accuracy, precision, and inter-day repeatability, were within the required limits. The method was applied to determine vancomycin and ceftazidime concentrations in CSF in five craniotomy patients.
- Published
- 2008