8 results on '"Sternberg, Katrin"'
Search Results
2. Development of Drug-Eluting Stents on the Basis of Genistein and Poly(L-lactide)
- Author
-
Sternberg, Katrin, Grabow, Niels, Löbler, Marian, Petzsch, Michael, Lipiecki, Janusz, Harder, Claus, Nienaber, Christoph, Schmitz, Klaus-Peter, Magjarevic, R., editor, Nagel, J. H., editor, Vander Sloten, Jos, editor, Verdonck, Pascal, editor, Nyssen, Marc, editor, and Haueisen, Jens, editor
- Published
- 2009
- Full Text
- View/download PDF
3. Development of Hydrophobized Alginate Hydrogels for the Vessel-Simulating Flow-Through Cell and Their Usage for Biorelevant Drug-Eluting Stent Testing
- Author
-
Semmling, Beatrice, Nagel, Stefan, Sternberg, Katrin, Weitschies, Werner, and Seidlitz, Anne
- Published
- 2013
- Full Text
- View/download PDF
4. Development of a vessel-simulating flow-through cell method for the in vitro evaluation of release and distribution from drug-eluting stents
- Author
-
Neubert, Anne, Sternberg, Katrin, Nagel, Stefan, Harder, Claus, Schmitz, Klaus-Peter, Kroemer, Heyo K., and Weitschies, Werner
- Subjects
- *
SURGICAL stents , *CELLS , *HYDROGELS , *HYDROPHOBIC surfaces - Abstract
Abstract: A novel in vitro dissolution test for drug-eluting stents (DES) based on the compendial flow-through cell was developed. The model contains an additional compartment simulating the vessel wall enabling the examination of drug release and distribution. The compartment consists of alginate hydrogel containing a central aperture forming the lumen for stent placement and media flow. The method was tested utilizing stents coated with hydrophilic (fluorescein sodium) and hydrophobic (triamterene) fluorescent model substances as well as the cytostatic drug doxorubicin hydrochloride and compared to standard dissolution methods. The results show the suitability of the developed method to observe drug release and distribution. The determination of model substance content in the compartments media, hydrogel and stent yielded differing distribution patterns for the model compounds and enabled the observation of redistribution processes. The dissolution profiles differed from the results of compendial dissolution testing. Besides lower endpoints and slower rises of media concentrations due to distribution into the hydrogel, the release rates from the stent coatings were altered. These findings emphasize the necessity to adapt dissolution testing for DES to the unique conditions influencing delivery to the vessel wall to learn more about local distribution and to anticipate the in vivo performance of DES. [Copyright &y& Elsevier]
- Published
- 2008
- Full Text
- View/download PDF
5. Iliac Anastomotic Stenting With a Sirolimus-Eluting Biodegradable Poly-L-Lactide Stent: A Preliminary Study After 6 Weeks.
- Author
-
Bünger, Carsten Michael, Grabow, Niels, Kröger, Christina, Lorenzen, Björn, Hauenstein, Karlheinz, Goosmann, Martin, Schmitz, Klaus-Peter, Kreutzer, Hans Jürgen, Lootz, Daniel, Ince, Hüseyin, Nienaber, Christoph A., Klar, Ernst, Schareck, Wolfgang, and Sternberg, Katrin
- Subjects
SURGICAL stents ,LACTIC acid ,RAPAMYCIN ,TOMOGRAPHY ,MEDICAL research - Abstract
Purpose: To assess technical feasibility and biocompatibility of a new biodegradable sirolimus eluting poly-L-lactide (PLLA) vascular anastomotic stent. Methods: A polytetrafluoroethylene bifurcated graft was implanted in 9 pigs through a midline abdominal incision. After transverse graft limb incision, 6 unloaded PLLAs, 6 sirolimus loaded PLLAs, and 6 unloaded stainless steel (316L) stents were randomly implant ed at both lilac anastomotic sites. Stents were deployed with a 6 mm balloon under direct vision without the use of angiography. Prior to sacrifice after 6 weeks, contrast-enhanced computed tomography (CT) was performed to determine patency of the target vessels. Stented segments were surgically explanted and processed for histology to measure the mean luminal diameter and intimal thickness and to assign vascular injury and inflammation scores. Results: No animals were lost during the study period. All stented graft limbs were patent on CT and histology. At the anastomotic sites and iliac arteries, the mean luminal diameter of SIR-PLLA stents (4.11±0.15 and 4.08±0.13 mm, respectively) were comparable to metal stents (4.23±0.35 and 4.21±0.26 mm, respectively), but significantly higher compared to unloaded PLLA stents [3.32±0.56 mm (p<0.001) and 3.29±0.39 mm (p=0.013), respectively]. At the iliac arteries, the mean intimal thickness was significantly lower with SIR-PLLA stents (0.09±0.02 mm) compared to unloaded PLLA stents (0.31±0.15 mm, p<0.001) and metal stents (0.19±0.04 mm, p=0.004). Vascular injury scores demonstrated only mild vascular trauma for all stents (SIR-PLLA: 0.42±0.63, PLLA: 0.51±0.62, metal: 0.50±0.62). Only mild inflammatory reaction was noted around SIR-PLLA stent struts (1.14±0.46), which was comparable to metal stents (1.27±0.45) but significantly lower than PLLA stents (1.79±0.56, p<0.001). Conclusion: SIR-PLLA stents showed comparable luminal diameter compared to metal stents, so incorporating sirolimus could reduce the inflammatory and neointimal response to PLLA stents. These findings need to be assessed with longer follow-up to confirm maintenance of efficacy. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
6. Impact of different tissue-simulating hydrogel compartments on in vitro release and distribution from drug-eluting stents.
- Author
-
Semmling, Beatrice, Nagel, Stefan, Sternberg, Katrin, Weitschies, Werner, and Seidlitz, Anne
- Subjects
- *
TISSUE physiology , *COLLOIDS in medicine , *CELL compartmentation , *DRUG-eluting stents , *CONTROLLED release drugs , *FLUORESCENCE - Abstract
In vitro drug release testing is an appropriate approach to identify critical parameters helping to predict drug release from drug-eluting stents (DES) prior to studying drug release behavior under in vivo conditions. Drug release and distribution from DES coated with a fluorescent model substance were studied in vitro using the vessel-simulating flow-through cell equipped with different long-term stable hydrogel compartments composed of agarose, polyacrylamide or poly(vinyl alcohol). The obtained experimental results were compared with the results of finite-element modeling obtained using experimentally determined diffusion coefficients and partition coefficients. In spite of differences regarding these parameters, experimental and mathematical data yielded only minor differences between the different gels regarding the release and distribution behavior and reasonable agreement between the modeling and the experiment was obtained. In an attempt to further elucidate the dosage form behavior, the diffusion coefficients in the gel as well as in the stent coating were systematically varied in the finite-element model. Changes in the diffusivity in the stent coating mainly impacted on the initial concentrations. Slower diffusion inside the hydrogel yielded a retarded elution from the stent coating and a higher model substance accumulation in the gel compartment at late time points. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
7. Sirolimus-Eluting Biodegradable Poly-l-Lactide Stent for Peripheral Vascular Application: A Preliminary Study in Porcine Carotid Arteries
- Author
-
Michael Bünger, Carsten, Grabow, Niels, Sternberg, Katrin, Kröger, Christina, Ketner, Lisa, Schmitz, Klaus-Peter, Kreutzer, Hans Jürgen, Ince, Hüseyin, Nienaber, Christoph A., Klar, Ernst, and Schareck, Wolfgang
- Subjects
- *
SURGICAL stents , *BLOOD vessels , *INFLAMMATION , *BIOMEDICAL materials - Abstract
Background: To assess technical feasibility and biocompatibility of a new Sirolimus (SIR)-eluting biodegradable poly-l-lactide (PLLA) stent for peripheral vascular application. Material and methods: In 15 pigs, both common carotid arteries (CCA) were surgically exposed and clamped in the proximal segment. After transverse incision, 12 316L stents, 12 unloaded and 6 SIR-loaded PLLA stents mounted on 6.0 × 40-mm balloon catheters were randomly implanted into the CCA and inflated to 8 bar. Angiographic equipment was not available. Stented CCA were explanted after 1 week (6 pigs; 316L versus PLLA) and 6 weeks (9 pigs; 316L versus PLLA versus SIR-PLLA), and processed for quantitative histomorphometry and estimation of vascular inflammation and injury scores. Results: No animals were lost during follow-up. All stents were patent on histological analysis without any signs of excessive recoiling or collapse. Unloaded PLLA stents showed decreased residual lumen area and increased neointimal area after 1 week (13.16 ± 0.34, 1.94 ± 0.26) and 6 weeks (11.57 ± 0.30, 2.85 ± 0.24) as compared with 316L stents (15.26 ± 0.13, 1.27 ± 0.41 and 13.99 ± 0.51, 1.54 ± 0.59). SIR-eluting stents demonstrated comparable neointimal area (1.75 ± 0.38) and 50% lower intimal thickness as compared with 316L stents after 6 weeks, but a slightly decreased residual lumen (13.06 ± 0.32) in the consequence of differences in strut thickness (PLLA, 270 μm; 316L, 155 μm). The vascular inflammation score against PLLA-stents could be reduced by Sirolimus. The vascular injury scores were low and similar in all groups. Conclusions: PLLA stents showed sufficient mechanical stability after porcine CCA stenting. By incorporation of Sirolimus, a significant reduction of the inflammatory and neointimal response to the PLLA stent was seen without systemic toxicity or thrombotic complications. These findings need to be assessed with longer follow-up to confirm maintenance of efficacy. The greater strut height of PLLA stents is a major limitation and requires modification. [Copyright &y& Elsevier]
- Published
- 2007
- Full Text
- View/download PDF
8. Examination of drug release and distribution from drug-eluting stents with a vessel-simulating flow-through cell
- Author
-
Seidlitz, Anne, Nagel, Stefan, Semmling, Beatrice, Grabow, Niels, Martin, Heiner, Senz, Volkmar, Harder, Claus, Sternberg, Katrin, Schmitz, Klaus-Peter, Kroemer, Heyo K., and Weitschies, Werner
- Subjects
- *
CONTROLLED release drugs , *DRUG solubility , *FINITE element method , *SURGICAL stents , *DRUG delivery systems , *MATHEMATICAL models - Abstract
Abstract: The recently introduced vessel-simulating flow-through cell offers new possibilities to examine the release from drug-eluting stents in vitro. In comparison with standard dissolution methods, the additional compartment allows for the examination of distribution processes and creates dissolution conditions which simulate the physiological situation at the site of implantation. It was shown previously that these conditions have a distinct influence on the release rate from the stent coating. In this work, different preparation techniques were developed to examine the spatial distribution within the compartment simulating the vessel wall. These methods allowed for the examination of diffusion depth and the distribution resulting in the innermost layer of the compartment simulating the vessel wall. Furthermore, the in vitro release and distribution examined experimentally were modelled mathematically using finite element (FE) methods to gain further insight into the release and distribution behaviour. The FE modelling employing the experimentally determined diffusion coefficients yielded a good general description of the experimental data. The results of the modelling also provided important indications that inhomogeneous coating layer thicknesses around the strut may result from the coating process which influence release and distribution behaviour. Taken together, the vessel-simulating flow-through cell in combination with FE modelling represents a unique method to analyse drug release and distribution from drug-eluting stents in vitro with particular opportunities regarding the examination of spatial distributions within the vessel-simulating compartment. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.