Search

A comparative oral pharmacokinetic study of five anthraquinones (aloe-emodin, emodin, rhein, chrysophanol and physcion) from the extract of Rheum palmatum L. was performed in normal and thrombotic focal cerebral ischemia (TFCI)-induced rats. The plasma samples were clarified through solid phase extraction prior to simultaneous determination of the anthraquinones with a validated high-performance liquid chromatography-fluorescence system. The results indicated that the Cmax, t(1/2) and AUC(0-t), of aloe-emodin, rhein, emodin and chrysophanol in TFCI-induced rats were nearly double, whereas the CL values were remarkably decreased (p < 0.05) over those of the normal rats. The plasma drug concentration-time data of five anthraquinones to rats fitted a two-compartment open model. The five anthraquinones in rat plasma were absorbed quickly and eliminated slowly in both groups. The obtained results could be helpful for evaluating the impact of the efficacy and safety of the drug in clinical applications., (Copyright © 2012 John Wiley & Sons, Ltd.)

A tris(2,2-bipyridyl)ruthenium(II) (Ru(bpy)₃²⁺)-based electrochemiluminescence (ECL) detection coupled with capillary electrophoresis (CE) method has been established for the sensitive determination of ephedrine for the first time. Under the optimized conditions [ECL detection at 1.15 V, 25 mmol/L phosphate buffer solution (PBS), pH 8.0, as running buffer, separation voltage 12.5 kV, 5 mmol/L Ru(bpy)₃²⁺ with 60 mmol/L PBS, pH 8.5, in the detection cell] linear correlation (r = 0.9987) between ECL intensity and ephedrine concentration was obtained in the range 6.0 × 10⁻⁸-6.0 × 10⁻⁶ g/mL. The detection limit was 4.5 × 10⁻⁹ g/mL (S:N = 3). The developed method was successfully applied to the analysis of ephedrine in human urine and the investigation of its interactions with three proteins, including bovine serum albumin (BSA), cytochrome C (Cyt-C) and myoglobin (Mb). The number of binding sites and the binding constants between ephedrine and BSA, Cyt-C and Mb were 8.52, 12.60, 10.66 and 1.55 × 10⁴  mol/L, 6.58 × 10³  mol/L and 1.59 × 10⁴  mol/L, respectively., (Copyright © 2011 John Wiley & Sons, Ltd.)

An effective, accurate and reliable method was developed for the simultaneous separation and determination of eight active components (baicalin, baicalein, sophoricoside, rutin, quercetin, genistein, pargyline and hydrochlorothiazide) in Chinese medicine 'JiangYaBiFeng' tablet (JYBF tablet) by high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD). Due to the different UV characteristic of these components, different wavelengths were selected for analysis of different analytes, such as 210nm for pargyline, 256nm for sophoricoside, rutin, quercetin and genistein, and 280nm for baicalin, baicalein and hydrochlorothiazide. Excellent linear behaviors over the investigated concentration ranges were observed with the values of R(2) higher than 0.9990 for all analytes. The recovery rates and relative standard deviation (RSD) for all analytes at three different concentrations were 94.9-104.7% and 1.23-3.00%, respectively. The validated method was successfully applied to the simultaneously determination of these active components in 'JiangYaBiFeng' tablet from different production batches, indicating that the proposed method in this paper was particularly suitable for the routine analysis of JYBF tablet and its quality control., (Copyright © 2011 Elsevier B.V. All rights reserved.)

The determination of trace lead in Huaiyao after separating and enriching with sulfhydryl cotton by FAAS is described in this paper. The enriching and eluting conditions were studied. The recovery of lead is 97.0%-102.0%. RSD is less than 4.3% sulfhydryl cotton is effective in eliminating the interference for common existing elements. The characteristics of this method is fast, simple and convenient. The determine results were consistent.

A new method--suspension sampling technique was applied to flame atomic absorption spectrometry and was successfully used to determine Cu, Zn, Fe in Huaiyao. Then studied the choice of suspension agent and elimination of chemical interference. The huaiyao sample was suspended in agar sol, and injected into air-acetylene flame by using standard addition method to determine Cu, Zn, Fe. Determined results were consistent with those obtained by HNO3 + H2SO4 + HClO4 method. The t-test showed that no obvious difference was found between the two methods. Displacement of HNO3 + H2SO4 + HClO4 method by suspension sampling method for the sample pretreatment is possible. This method is convenient, rapid and accurate.

This paper reports that the sulfur compounds of traditional Chinese medicine were reduced to S2- by Auger reagent, and determined by cold atomic fluorescence. At the same time the trace mercury was determined. The conditions the for quantitative determination of Hg2+ and S2- were obtained. The linear range of the determination is 11.20-55.60 mg.mL-1, the correlation coefficient is 0.999 78. In this method, little reagent is needed, the treatment of the sample is simple, the process of determination is convenient, and the sensitivity is high.

Chemical pattern recognition was applied to three Chinese herbal medicines from the genus Stephania Lour., viz. S. kwangsiensis Lo, S. viridiflavens Lo and M. Yang and S. mashanica Lo and B.N. Chang. Based on the chemical features obtained from HPLC, SIMCA program was carried out and the results showed that the classification accuracy was 100%. In addition, the obtained features showed three major classes by NLM. The results of both methods were consistent with those of plant taxonomical identification. It suggested that chemical pattern recognition could be a helpful method to classify and identify Chinese herbal medicines.