1. Simultaneous determination of seven bioactive components in Guizhi Fuling capsule by microwave-assisted extraction combined with ultra performance liquid chromatography tandem mass spectrometry.
- Author
-
Sui Y, Zhao LS, Wang ZZ, Zhao YT, Xiao W, and Xiong ZL
- Subjects
- Acetophenones analysis, Amygdalin analysis, Bridged-Ring Compounds analysis, Capsules, Cinnamates analysis, Gallic Acid analysis, Glucosides analysis, Microwaves, Monoterpenes analysis, Quality Control, Reproducibility of Results, Tandem Mass Spectrometry standards, Triterpenes analysis, Chemical Fractionation methods, Chromatography, High Pressure Liquid methods, Drugs, Chinese Herbal analysis, Drugs, Chinese Herbal chemistry, Tandem Mass Spectrometry methods
- Abstract
A simple, rapid and reliable microwave-assisted extraction (MAE) combined with ultra performance liquid chromatography tandem mass spectrometry method was developed for simultaneous determination of the seven bioactive constituents in Guizhi Fuling capsule (GFC), namely gallic acid, amygdalin, albiflorin, paeoniflorin, paeonol, cinnamic acid and pachymic acid, respectively. The operation of MAE optimised through orthogonal array design experiment was performed at 80°C for 10 min with methanol-water (70:30, v/v) as the extracting solvent. The method was validated including intra- and inter-day precision, repeatability and stability, with relative standard deviation less than 3.9%, 3.3%, 4.4% and 3.1%, respectively. All analytes showed the good linearity (r >0.999), and their average recoveries varied between 98.2% and 101.2%. The results indicated that this method was simple, effective and suitable for the quality control of GFC.
- Published
- 2016
- Full Text
- View/download PDF