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Objective: To investigate the effects of Shiquanyuzhentang (Traditional Chinese Medicine) on the immunologic function of tu-mor-bearing mice and its mechanism of antitumor., Methods: Thirty SPF grade male Kunming mice transplanted with H 22 hepatocellular carci-noma were divided into three groups randomly:model group,positive control group and Shiquanyuzhentang group( n =10). Another 10 mice were selected as normal control group. Normal control group and model group received normal saline and distilled water supplementation by 10 mL/kg everyday. Positive control group and Shiquanyuzhentang group received Shengyi(80 mg/ml)and Shiquanyuzhentang decoction at the doses of 8 g/kg and 18 g/kg respectively everyday. After 14 days of continuous administration, the mice were killed and the thymus, spleen index, tumor inhibition rate,peripheral blood leukocytes,lymphocyte content,cell percentage of T cell subsets CD3, CD4, CD8,interleukin 2 (IL-2)in serum,tumor necrosis factor-α(TNF-α),interferon β(IFN-β) content,lymphocyte proliferation ability and NK cell were measured., Results: Compared with normal control group, the weight of mice and thymus and spleen index of the Shiquanyuzhentang group were increased significantly( P <0.05);leukocyte and lymphocyte CD3、CD4、CD8 and TNF-α were increased greatly( P <0. 05),IL-2 and IFN-β were de-creased significantly( P <0.05). Compared with model group,thymus and spleen index of Shiquanyuzhentang group were increased significant-ly( P <0.05);leukocyte and lymphocyte CD3、CD4、IL-2、IFN-β and TNF-α of Shiquanyuzhentang group were increased significantly( P <0. 05),CD8 content was decreased significantly( P <0.05);NK cell and lymphocyte proliferation were increased significantly( P <0.05)., Conclusions: Shiquanyuzhentang can promote the growth of immune organs in mice bearing H 22 , enhance immune function and is beneficial to the recovery of tumor body.

Three indole alkaloid glycosides, strobilanthosides A-C (1-3), two known indole alkaloid glucosides (4 and 5), and five phenylethanoid glycosides (8-10) were isolated from the aerial parts of Strobilanthes cusia. The structures of the new compounds were elucidated by spectrometric analysis, and the absolute configurations of 1 and 2 were established by ECD spectrocsopy. N'-β-d-Glucopyranosylindirubin (5) showed weak antibacterial activity (MIC 62.5-125 μM) against Staphylococcus aureus.

Objective: To investigate the chemical constituents of Euphorbia helioscopia and their antitumor activities., Methods: Normal phase silica gel, RP-18 silica gel and Sephadex LH-20 column chromatographies combined with recrystallization were used to isolate and purify the constituents. Their structures were identifided by spectroscopic methods, including 1H-NMR, 13C-NMR, ESI-MS and EI-MS. And the antitumor activities of some of chemical constituents in vitro were detected by sulphorhodamine B protein staining., Results: Nine compounds were isolated and their structures were identified as euphohelioscopin A (1), euphoscopin (2), 9, 19-cyclolanost-23E-ene-3, 25-diol (3), euphoscopin C (4), euphornin A (5), euphoheliosnoid A (6), ent-kaurane-3-oxo-16beta, 17-diol (7), 9, 19-cyclolanost-25-ene-3beta, 22-diol (8) and helioscopinolide A(9) Compound 9 showed effect on inhibiting the cell proliferations of MCF-7 cell line., Conclusion: Compounds 3, 7, 8 and 9 are obtained from this plant for the first time, and compound 9 shows the potential antitumor activity.

A new complex natural product with a C39 skeleton, named nudibaccatumone, and the known sesquiterpenes (+)-spathulenol, (-)-4β,10α-aromadendranediol, and ent-T-muurolol, as well as the phenylpropanoid hydroxychavicol, were isolated from the aerial parts of Piper nudibaccatum. The structure and absolute configuration of nudibaccatumone were elucidated using spectroscopic methods and ECD calculations. A 1,8-Michael addition reaction and an intermolecular, inverse electron demand Diels-Alder reaction are proposed as the key steps in the biosynthesis of nudibaccatumone.

Phytochemical study of the roots of Trigonostemon thyrsoideum led to the isolation of four new oxygenated daphnane-type diterpenoids, trigonosins A-D (1-4), and two new modified daphnanes, trigonosins E and F (5 and 6). The structures and relative configurations were elucidated on the basis of extensive spectroscopic analysis, including 1D and 2D NMR experiments. All compounds isolated were evaluated for their cytotoxicity against HL-60, A549, and MCF-7 human cancer cell lines.

Eight new pyrrolidinoindoline alkaloids (1-8) were isolated from the whole plant of Selaginella moellendorfii. Their structures were determined by mass spectrometry, 1D and 2D NMR spectroscopy, and chemical interconversions. These alkaloids have a 3-carboxybut-2-enyl group at C-3a and two methyl groups at N-8. The possible biogenetic route from selaginellic acid (1) to neoselaginellic acid (6) was postulated and chemically mimicked. Tautomerization between 6 and 6a was observed. Selected compounds were evaluated for antibacterial, cytotoxic, and acetylcholinesterase inhibitory activities.

Four new trijugin-type limonoids, cipatrijugins A-D (1-4), together with the known cipadesin A (5), were isolated from the leaves of Cipadessa cinerascens, and their structures were elucidated on the basis of spectroscopic and computational methods. The ability of compounds 1-5 to inhibit the growth of the A549 and K562 tumor cell lines was evaluated.