1. IL-17 is a protection effector against the adherent-invasive Escherichia coli in murine colitis.
- Author
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Zhang, Hai-jia, Xu, Bin, Wang, Hu, Xu, Bing, Wang, Guo-dong, Jiang, Ming-zuo, Lei, Chao, Ding, Mei-ling, Yu, Peng-fei, Nie, Yong-zhan, Wu, Kai-chun, Sha, Su-mei, and Li, Meng-bin
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INTERLEUKIN-17 , *ESCHERICHIA coli , *COLITIS , *INFLAMMATORY bowel diseases , *IMMUNOHISTOCHEMISTRY , *POLYMERASE chain reaction - Abstract
Inflammatory bowel disease (IBD) is caused by aberrant immune responses to the gut microbiota. Among the gut microbiota, adherent-invasive Escherichia Coli (AIEC) is thought to be the pathogen through invading the intestinal epithelial cells and causing inflammation. IL-17 secretion increase, induced by enhanced bacterial adhesion to the intestine epithelium, could on one hand protect the mucosa, but on the other hand, over amount of IL-17 initializes inflammation reactions that in turn damages the mucosa. The relationship between IL-17 and AIEC is still unclear. In this study, we tried to elucidate the function of IL-17 in AIEC-mediated colitis. Wild type (WT) and IL-17 knockout (IL-17 KO) mice were inoculated with AIEC strain E. coli LF82 and treated with dextran sodium sulphate (DSS). Histological examination of the colon was performed. Mucosa damage was assessed and scored. IL-22 and IL-17 in colon tissues were detected by ELISA, qPCR and immunohistochemistry methods. Transient AIEC colonization in IL-17 KO mice resulted in increased intestinal epithelial damage, systemic bacterial burden and mortality compared with WT controls. Moreover, IL-17 is required for the induction of IL-22 in the experimental animal models during AIEC strain E. coli LF82 colonization. These results indicate IL-17 plays a protective role in AIEC strain E. coli LF82 induced colitis by promoting IL-22 secretion. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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