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In this study we examined the effects histopathologic and immunohistochemical of xylene inhalation in rats by using light microscopy. Adult wistar albino rats were used in this study. Eight rats were in control group and 8 rats were in the experimental group. The experimental group was exposed to 300 ppm formaldehyde 35 min/day, 5 days/week for 8 weeks. The lining epithelium of respiratory mucosa showed a loss of ciliated cells with metaplasia of goblet cells, hyperplasia of squamous cells and edema, inflamation in sub epithelial area). In the group treated xylene. Disruption of cell-cell contact was observed. Weak expression of E-cadherin was observed between cells. The vascular endothelium of capillaries and venoles showed intense immunostaining for VEGF. Se examinó el efecto histopatológico e inmunohistoquímico de la inhalación de xileno en ratas mediante el uso de microscopía de luz. Se utilizaron ratas albinas Wistar adultas. Ocho ratas formaron parte del grupo control y 8 del grupo experimental. El grupo experimental fue expuesto a 300 ppm de formaldehído, 35 min/día, 5 días/semana, durante 8 semanas. El epitelio de revestimiento de la mucosa respiratoria mostró una pérdida de células ciliadas con metaplasia de células caliciformes, hiperplasia de células escamosas y edema, con inflamación en la zona subepitelial. En el grupo tratado con xileno se observó una interrupción del contacto célula-célula. Se observó una débil expresión de E-cadherina entre las células. El endotelio vascular de los capilares y vénulas mostraron intensa inmunotinción de VEGF.

The purpose of present study was to investigate effects of nicotine on follicular development in the neonatal rats. In this study, 14 adult Sprague-Dawley rats weighing 230-260 g (±10 g) were used as experimental animal. After detecting pregnancies with vaginal smear method, pregnant rats were two equal groups were separated. The pregnant females were treated with the nicotine for 21 days and litters were sacrificed at first day of birth. The ovaries of litters were processed through routine paraffin embedding method, serially sectioned by microtome, stained with hematoxylin-Eosin and immunohistochemical technique investigated by light microscope.In the ovary of group treated nicotine, Preventing follicular development was observed in granular degeneration and luteal cells. It was also observed in inflammation and bleeding vessels. Immunohistochemical localization of vimentin was restricted to the granulosa cells and stromal area. Nicotine treatment group in granular cells and luteal cells in E-cadherin expression was weak. We concluded that nicotine might affect the cellular junctions in the ovarian follicular development. The high intensity in the staining with vimentin observed in the granulosa cells of the follicles is probably due to functional changes that occur during the process of cystogenesis. © 2016, E-Century Publishing Corporation. All rights reserved.

The purpose of this study is to examine the changes in the umbilical cord in women diagnosed with gestational diabetes mellitus In this study, as a control group human placental tissues from normotensive pregnancies was collected from diabetic women at 2835 weeks of gestation. Gestational diabetes (n= 20) and normal umbilical cord (n= 20) for a total of 40 units were received.GDM groups compared to the control group was significantly higher values was detected (p

OBJECTIVE: To investigate the effects of Potentilla fulgens as a prophylactic agent on ischemia/reperfusion (I/R) injury in the rat ovary. STUDY DESIGN: A total of 32 Wistar rats were divided into 4 equal groups: (I) sham, (II) ischemia, (III) ischemia+reperfusion, and (IV) I/R+Potentilla fulgens. In groups I and II, ovary torsion was not performed and no drug was administered. In group III, 1 hour of ischemia and 2 hours of reperfusion were performed and no drug was given. Group IV received 400 mg/kg/day Potentilla fulgens intraperitoneally 5 days before I/R injury. RESULTS: The detorsion group showed preantral ovarian follicles and corpus luteum around the blood vessels and positive expression of vascular endothelial growth factor (VEGF). In the Potentilla fulgens group (IV) the stromal vascular endothelium with weak expression of VEGF was detected in small areas, and the ovarian follicles and the corpus luteum showed negative expression of VEGF. In the detorsion group the theca cells and apoptotic cells in preantral follicles showed positive expression of E-cadherin in the ovarian surface epithelium. Moreover, the E-cadherin expression was found to be positive in terms of follicular development, theca cells, granulosa cells, and corpus luteum. Potentilla fulgens, given after ischemic injury and apoptosis, was seen to decrease the effect of Bcl-2 expression. CONCLUSION: These results provide compelling evidence that the expression of E-cadherin in the ovary is an important component of ovarian function. © Science Printers and Publishers, Inc.