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Urban biodiversity and ecosystem services depend on the quality, quantity, and connectivity of urban green areas (UGAs), which are crucial for enhancing urban livability and resilience. However, assessing these connectivity metrics in urban landscapes often suffers from outdated land cover classifications and insufficient spatial resolution. Spectral data from Earth Observation, though promising, remains underutilized in analyzing UGAs' connectivity. This study tests the impact of dataset choices on UGAs' connectivity assessment, comparing land cover classification (Urban Atlas) and spectral data (Normalized Difference Vegetation Index, NDVI). Conducted in seven European cities, the analysis included 219 UGAs of varying sizes and connectivity levels, using three connectivity metrics (size, proximity index, and surrounding green area) at different spatial scales. The results showed substantial disparities in connectivity metrics, especially at finer scales and shorter distances. These differences are more pronounced in cities with contiguous UGAs, where Urban Atlas faces challenges related to typology issues and minimum mapping units. Overall, spectral data provides a more comprehensive and standardized evaluation of UGAs' connectivity, reducing reliance on local typology classifications. Consequently, we advocate for integrating spectral data into UGAs' connectivity analysis to advance urban biodiversity and ecosystem services research. This integration offers a comprehensive and standardized framework for guiding urban planning and management practices. [ABSTRACT FROM AUTHOR]