1. Pooling for SARS-CoV2 Surveillance: Validation and Strategy for Implementation in K-12 Schools
- Author
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Alexandra M. Simas, Jimmy W. Crott, Chris Sedore, Augusta Rohrbach, Anthony P. Monaco, Stacey B. Gabriel, Niall Lennon, Brendan Blumenstiel, and Caroline A. Genco
- Subjects
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Population ,Pooling ,Pilot Projects ,Specimen Handling ,Single test ,Repeated testing ,Environmental health ,Humans ,Medicine ,education ,education.field_of_study ,University community ,Schools ,SARS-CoV-2 ,business.industry ,screening ,Public Health, Environmental and Occupational Health ,COVID-19 ,pooled testing methodology ,Limiting ,Brief Research Report ,Cost savings ,RT-PCR assay ,RNA, Viral ,Public Health ,Public aspects of medicine ,RA1-1270 ,business - Abstract
Repeated testing of a population is critical for limiting the spread of the SARS-CoV-2 virus and for the safe reopening of educational institutions such as K-12 schools and colleges. Many screening efforts utilize the CDC RT-PCR based assay which targets two regions of the novel Coronavirus nucleocapsid gene. The standard approach of testing each person individually, however, poses a financial burden to these institutions and is therefore a barrier to using testing for re-opening. Pooling samples from multiple individuals into a single test is an attractive alternate approach that promises significant cost savings - however the of specificity and sensitivity of such approaches needs to be assessed prior to deployment. To this end, we conducted a pilot study to evaluate the feasibility of analyzing samples in pools of eight by the established RT-PCR assay. Participants (1,576) were recruited from amongst the Tufts University community undergoing regular screening. Each volunteer provided two swabs, one analyzed separately and the other in a pool of eight. Because the positivity rate was very low, we spiked approximately half of the pools with laboratory-generated swabs produced from known positive cases outside the Tufts testing program. The results of pooled tests had 100% correspondence with those of their respective individual tests. We conclude that pooling eight samples does not negatively impact the specificity or sensitivity of the RT-PCR assay and suggest that his approach can be utilized by institutions seeking to reduce surveillance costs.
- Published
- 2020