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Your search keyword '"Z. Kurzová"' showing total 4 results
Start Over Author "Z. Kurzová" Topic ehrlichiosis
4 results on '"Z. Kurzová"'

1. [Serology of Lyme borreliosis and human granulocytic ehrlichiosis in 2005-2010].

Author

Balátová P, Kurzová Z, and Hulínská D

Subjects
Anaplasma phagocytophilum immunology, Female, Humans, Male, Serologic Tests, Antibodies, Bacterial analysis, Borrelia immunology, Ehrlichia immunology, Ehrlichiosis diagnosis, Lyme Disease diagnosis
Abstract

The subject of this study is serological screening of blood and CSF (cerebrospinal fluid) samples for the presence of borrelial and ehrlichial antibodies. A total of 165 patients suspected to be at risk of Lyme disease were tested. Indirect immunofluorescence and enzyme immunoassay were used as diagnostic methods. Ehrlichial antibodies were detected in 36 (21.8%) patients. Borrelial antibodies were found in 70 samples (42.4%). The widening range of tick-borne diseases brings about the need for more data on these zoonoses.

Published
2011

2. Detection of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in dogs in the Czech Republic.

Author

Kybicová K, Schánilec P, Hulínská D, Uherková L, Kurzová Z, and Spejchalová S

Subjects
Anaplasma phagocytophilum immunology, Animals, Antibodies, Bacterial, Borrelia burgdorferi immunology, Czech Republic epidemiology, Dog Diseases blood, Dog Diseases epidemiology, Dogs, Ehrlichiosis blood, Ehrlichiosis epidemiology, Ehrlichiosis microbiology, Female, Immunoglobulin G, Immunoglobulin M, Lyme Disease blood, Lyme Disease epidemiology, Lyme Disease microbiology, Male, Polymerase Chain Reaction veterinary, Ticks microbiology, Anaplasma phagocytophilum isolation & purification, Borrelia burgdorferi isolation & purification, Dog Diseases microbiology, Ehrlichiosis veterinary, Lyme Disease veterinary
Abstract

The aim of this study is to present molecular, serologic, and clinical findings for dogs that were naturally infected with Anaplasma phagocytophilum or Borrelia burgdorferi sensu lato (s. l.) in the Czech Republic. This data can provide information relevant to human infection. In total, blood samples from 296 dogs and 118 engorged ticks were examined. Samples were tested for A. phagocytophilum using polymerase chain reaction (PCR) amplification, nested PCR, and direct sequencing of the 16S rDNA, and for B. burgdorferi s. l. using PCR amplification of the 16S rDNA and restriction fragment length polymorphism analysis of the 5S-23S rDNA intergenic spacer. In addition, blood samples were screened for antibodies to these bacteria. Ten (3.4%) dogs were PCR-positive for A. phagocytophilum. Morulae of A. phagocytophilum in granulocytes were found in two of these dogs. Nine of the PCR-positive dogs had clinical signs related to anaplasmosis. Statistically significant differences in the PCR detection rates were found between breeds and between symptomatic and asymptomatic dogs. Infection with Borrelia garinii was detected by PCR in a dog with meningoencephalitis. DNA of A. phagocytophilum and B. burgdorferi s. l. (B. garinii or Borrelia afzelii) was detected in 8.5% and 6.8% of ticks, respectively. Immunoglobulin (Ig) G seropositivity to A. phagocytophilum was 26%. Significant differences were found with respect to breed and gender. IgM and IgG antibodies to B. burgdorferi s. l. were detected in 2.4% and 10.3% of dogs, respectively. Our findings suggest that the exposure to B. burgdorferi s. l. exists in dogs in the Czech Republic, and exposure to A. phagocytophilum is common.

Published
2009
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3. [Serology of Lyme borreliosis and human granulocytic ehrlichiosis in 2005-2010]

Author

P, Balátová, Z, Kurzová, and D, Hulínská

Subjects
Male, Lyme Disease, Borrelia, Ehrlichia, Ehrlichiosis, Humans, Female, Serologic Tests, Antibodies, Bacterial, Anaplasma phagocytophilum
Abstract

The subject of this study is serological screening of blood and CSF (cerebrospinal fluid) samples for the presence of borrelial and ehrlichial antibodies. A total of 165 patients suspected to be at risk of Lyme disease were tested. Indirect immunofluorescence and enzyme immunoassay were used as diagnostic methods. Ehrlichial antibodies were detected in 36 (21.8%) patients. Borrelial antibodies were found in 70 samples (42.4%). The widening range of tick-borne diseases brings about the need for more data on these zoonoses.

Published
2011

4. Identification of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in patients with erythema migrans

Author

H. Dřevová, Daniela Vaňousová, J. Votýpka, L. Uherkova, Z. Kurzová, Jana Hercogová, V. Hulínský, and Dagmar Hulínská

Subjects
Adult, Male, Human granulocytic anaplasmosis, Adolescent, Lipoproteins, Placenta, HL-60 Cells, Microbiology, Diagnosis, Differential, Lyme disease, Pregnancy, Borrelia, parasitic diseases, medicine, Humans, Anaplasma, Borrelia burgdorferi, Pregnancy Complications, Infectious, Aged, Lyme Disease, biology, Ehrlichiosis, General Medicine, Middle Aged, bacterial infections and mycoses, medicine.disease, biology.organism_classification, Anaplasma phagocytophilum, Virology, Antibodies, Bacterial, Antigens, Surface, Bacterial Vaccines, bacteria, Erythema migrans, Lyme disease microbiology, Erythema Chronicum Migrans, Female, Bacterial Outer Membrane Proteins
Abstract

Anaplasma phagocytophilum has been first isolated from the blood of two Czech patients simultaneously with a cultivation of Borrelia burgdorferi sensu lato from their erythema migrans lesions. Cultivation of different Borrelia spp. from 12 erythema migrans biopsies, from 2 blood, one liquor and one placenta sample in BSK-H medium was successful. Adapted conventional methods targeting 16S rRNA and OspA genes for real-time polymerase chain reaction (PCR) and partial sequencing of these genes together with microscopical examinations of the blood smears provided a direct detection of the B. afzelii, B. burgdorferi, B. garinii, B. valaisiana and B. bissettii in the skin, B. garinii in the blood, placenta and liquor in 24 (36.3 %) patients, and A. phagocytophilum in 10 (15 %) patients with erythema migrans. Positive indirect IgM immunofluorescence against Anaplasma sp. was obtained in 7 cases, specific IgG antibodies were detected in 12 patients. Three women suffering from erythema migrans in the first trimester had positive PCR for Anaplasma and/or for Borrelia in the blood and two of them, later, in the placenta. Interpretation of laboratory data can bring important contribution to establishing the role of Anaplasma sp. in erythema migrans and forming the principle of precaution with laboratory diagnosis during pregnancy which always should be reflected in the resistance of Anaplasma sp. toward penicillins.

Published
2008

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