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3 results on '"Jiantao Pang"'

1. A nitric oxide biosensor based on the multi-assembly of hemoglobin/montmorillonite/polyvinyl alcohol at a pyrolytic graphite electrode

Author

Genxi Li, Chunhai Fan, Jiantao Pang, Ting Chen, and Xinjian Liu

Subjects
Hot Temperature, Manufactured Materials, Biomedical Engineering, Biophysics, Analytical chemistry, Biosensing Techniques, Nitric Oxide, Polyvinyl alcohol, Sensitivity and Specificity, chemistry.chemical_compound, Hemoglobins, Coated Materials, Biocompatible, Electrochemistry, Graphite, Pyrolytic carbon, Electrodes, Detection limit, Chemistry, Reproducibility of Results, General Medicine, Equipment Design, Equipment Failure Analysis, Montmorillonite, Linear range, Polyvinyl Alcohol, Electrode, Bentonite, Biosensor, Biotechnology, Nuclear chemistry
Abstract

Direct electron transfer of hemoglobin (Hb) can be achieved in a Hb/montmorillonite (MMT)/polyvinyl alcohol multi-assembly at a pyrolytic graphite (PG) electrode. Accordingly, a novel nitric oxide (NO) biosensor is proposed. The reduction of NO is observed at a potential of approximately -783 mV (vs. SCE) at pH 5.5. At optimum pH, this biosensor shows a wide linear range of 1.0x10(-6)-2.5x10(-4) mol/l with a detection limit of 5.0x10(-7) mol/l. The sensor-to-sensor reproducibility is good consideringmacr; a relative standard deviation of 3.5% in five independent determinations at 5.0x10(-5) mol/l NO. The modified electrode is conveniently constructed and durable in long-term operations.

Published
2003

2. A reagentless nitric oxide biosensor based on haemoglobin/polyethyleneimine film

Author

Genxi Li, Xinjian Liu, Libin Shang, and Jiantao Pang

Subjects
Inorganic chemistry, Biomedical Engineering, Bioengineering, Biosensing Techniques, Electrochemistry, Nitric Oxide, Applied Microbiology and Biotechnology, Catalysis, Hemoglobins, Drug Discovery, Animals, Polyethyleneimine, Pyrolytic carbon, Electrodes, Detection limit, Dose-Response Relationship, Drug, Chemistry, Process Chemistry and Technology, General Medicine, Hydrogen-Ion Concentration, Electrode, Molecular Medicine, Cattle, Cyclic voltammetry, Biosensor, Biotechnology, Chemically modified electrode
Abstract

An unmediated NO biosensor was prepared by co-immobilizing haemoglobin with polyethyleneimine at a pyrolytic graphite electrode surface. Haemoglobin exhibits direct electrochemistry in the membrane, and this modified electrode shows high stability and catalytic activity towards the reduction of NO. A linear relationship between the catalytic current and the concentration of NO was observed in the concentration ranges 1.0 x 10 -7 -8.0 x 10 -6 M and 1.0 x 10 -5 -2.5 x 10 -5 M, with a detection limit of 4.0 x 10 -8 M.

Published
2003

3. Nitric oxide biosensors based on Hb/phosphatidylcholine films

Author

Pingping Shen, Jiantao Pang, Chunhai Fan, Genxi Li, and Dexu Zhu

Subjects
Detection limit, Analytical chemistry, Electrons, Membranes, Artificial, Biosensing Techniques, Electrochemistry, Nitric Oxide, Analytical Chemistry, chemistry.chemical_compound, Hemoglobins, chemistry, Phosphatidylcholine, Electrode, Phosphatidylcholines, Animals, Reactivity (chemistry), Cattle, Pyrolytic carbon, Selectivity, Biosensor, Oxidation-Reduction, Algorithms, Nuclear chemistry
Abstract

Hemoglobin (Hb) was entrapped in a phosphatidylcholine (PC) film and immobilized at a pyrolytic graphite (PG) electrode surface. Its electron-transfer reactivity and enzyme activity were characterized by employing electrochemical methods. It was observed that Hb exhibited direct electrochemistry as well as enzyme-like activity towards the electrocatalytic reduction of NO in PC film. An unmediated, reagentless nitrogen oxide (NO) biosensor was accordingly prepared. Experimental results revealed that the peak current related to NO was linearly proportional to its concentration in the range of 1.0 x 10(-7)-3.0 x 10(-4) mol/L. The detection limit was estimated to be 1.0 x 10(-7) mol/L. Considering its good stability, nice selectivity and easy construction, this biosensor shows great promise for the rapid determination of traces of NO.

Published
2002

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