1. A chitinase with antifungal activity from the mung bean
- Author
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Ye, Xiuyun and Ng, Tzi Bun
- Subjects
- *
ELECTROPHORESIS , *FUSARIUM oxysporum , *GEL electrophoresis , *HYDROGEN-ion concentration - Abstract
Abstract: A chitinase with antifungal activity was isolated from mung bean (Phaseolus mungo) seeds. The procedure entailed aqueous extraction, (NH4)2SO4 precipitation, ion-exchange chromatography on CM-Sepharose, high-performance liquid chromatography (HPLC) on Poros HS-20, and gel filtration on Sephadex G-75. The protein exhibited a molecular mass of 30.8kDa in SDS–polyacrylamide gel electrophoresis. Its pI was 6.3 as determined by isoelectric focusing. The specific activity of the chitinase was estimated to be 3.81U/mg. The enzyme expressed its optimum activity at pH 5.4 and was stable from 40 to 50°C. It exerted antifungal action toward Fusarium solani, Fusarium oxysporum, Mycosphaerella arachidicola, Pythium aphanidermatum, and Sclerotium rolfsii. [Copyright &y& Elsevier]
- Published
- 2005
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