8 results on '"Narfström, Kristina"'
Search Results
2. Amax is the Best a-wave Measure for Classifying Abyssinian Cat Rod/Cone Dystrophy
- Author
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Vaegan and Narfström, Kristina
- Published
- 2005
- Full Text
- View/download PDF
3. Clinical electroretinography in the dog with ganzfeld stimulation: A practical method of examining rod and cone function
- Author
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Narfström, Kristina, Andersson, Björn-Erik, Andreasson, Sten, and Gouras, Peter
- Published
- 1995
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4. Enzyme replacement therapy delays pupillary light reflex deficits in a canine model of late infantile neuronal ceroid lipofuscinosis.
- Author
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Whiting, Rebecca E. H., Narfström, Kristina, Yao, Gang, Pearce, Jacqueline W., Coates, Joan R., Castaner, Leilani J., Jensen, Cheryl A., Dougherty, Brittanie N., Vuillemenot, Brian R., Kennedy, Derek, O'Neill, Charles A., and Katz, Martin L.
- Subjects
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CANIDAE , *NEURONAL ceroid-lipofuscinosis , *GENETIC disorders , *RETINAL degeneration , *VISION disorders , *COGNITION , *ANIMAL models in research - Abstract
Late-infantile neuronal ceroid lipofuscinosis (CLN2 disease) is a hereditary neurological disorder characterized by progressive retinal degeneration and vision loss, cognitive and motor decline, seizures, and pronounced brain atrophy. This fatal pediatric disease is caused by mutations in the CLN2 gene which encodes the lysosomal enzyme tripeptidyl peptidase-1 (TPP1). Utilizing a TPP1-/- Dachshund model of CLN2 disease, studies were conducted to assess the effects of TPP1 enzyme replacement administered directly to the CNS on disease progression. Recombinant human TPP1 (rhTPP1) or artificial cerebrospinal fluid vehicle was administered to CLN2-affected dogs via infusion into the CSF. Untreated and vehicle treated affected dogs exhibited progressive declines in pupillary light reflexes (PLRs) and electroretinographic (ERG) responses to light stimuli. Studies were undertaken to determine whether CSF administration of rhTPP1 alters progression of the PLR and ERG deficits in the canine model. rhTPP1 administration did not inhibit the decline in ERG responses, as rhTPP1 treated, vehicle treated, and untreated dogs all exhibited similar progressive and profound declines in ERG amplitudes. However, in some of the dogs treated with rhTPP1 there were substantial delays in the appearance and progression of PLR deficits compared with untreated or vehicle treated affected dogs. These findings indicate that CSF administration of TPP1 can attenuate functional impairment of neural pathways involved in mediating the PLR but does not prevent loss of retinal responses detectable with ERG. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
5. Pupillary light reflex deficits in a canine model of late infantile neuronal ceroid lipofuscinosis.
- Author
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Whiting, Rebecca E.H., Narfström, Kristina, Yao, Gang, Pearce, Jacqueline W., Coates, Joan R., Castaner, Leilani J., and Katz, Martin L.
- Subjects
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CANIDAE , *NEURONAL ceroid-lipofuscinosis , *GENETIC disorders , *NEUROLOGICAL disorders , *RETINAL degeneration , *BLINDNESS , *COGNITIVE ability , *CEREBRAL atrophy - Abstract
Abstract: Late-infantile neuronal ceroid lipofuscinosis (CLN2) is a hereditary neurological disorder characterized by progressive retinal degeneration and vision loss, cognitive and motor decline, seizures, and pronounced brain atrophy. The progressive loss of neurological functions eventually leads to death, usually by the early teenage years. Utilizing a canine model of CLN2, therapeutic studies to inhibit the brain and retinal degenerations are currently under way. Using this dog model, studies were undertaken to compare quantitative assessments of the pupillary light reflex (PLR) and electroretinography (ERG) as tools for evaluating the effects of the disease on retinal function. The PLR and ERG were recorded in normal and CLN2-affected Dachshunds at 2 month intervals between the ages of 4 and 10 months. Using custom instrumentation for quantitative PLR assessments, a series of white light stimuli of varying intensity was used to elicit pupil constriction, and pupil images were recorded using continuous infrared illumination and an infrared-sensitive camera. Electroretinography was used to evaluate retinal function in the same dogs. As the disease progressed, affected dogs exhibited progressive and profound declines in ERG amplitudes under both scotopic and photopic conditions. With low intensity light stimuli, CLN2 was also accompanied by progressive deficits in the PLR. Changes in the PLR to dim light stimuli included significant deficits in latency, constriction velocity, constriction amplitude, and redilation velocity. However, despite the almost complete loss of detectable ERG responses by disease end stage, the PLR to bright stimuli was well preserved throughout the disease progression. These findings demonstrate that the PLR is much more sensitive than the ERG in detecting residual retinal function in animal models of retinal degenerative disease. The preservation of the PLR in dogs with profoundly depressed ERGs correlates with a preservation of visually-mediated behavior even late in the disease progression. Quantitative analysis of the PLR has potential as a biomarker in animal models of retinal degenerative diseases and in evaluating the efficacy of therapeutic interventions in preserving retinal function. [Copyright &y& Elsevier]
- Published
- 2013
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- View/download PDF
6. Electroretinography in the western gray kangaroo ( Macropus fuliginosus).
- Author
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Labelle, Amber L., Hamor, Ralph E., Narfström, Kristina, and Breaux, Carrie B.
- Subjects
ELECTRORETINOGRAPHY ,WESTERN grey kangaroo ,SPECIES ,KETAMINE ,ZOOLOGICAL research - Abstract
Objective To perform electroretinography on normal anesthetized western gray kangaroos ( Macropus fuliginosus). Animals studied Six captive western gray kangaroos. Procedures The kangaroos were anesthetized using a combination of ketamine and medetomidine via a remote drug delivery system, then were maintained on isoflurane after endotracheal intubation and reversal of the medetomidine with atipamazole. After a minimum of 20 min of dark adaptation, electroretinograms were obtained using a handheld electroretinography (ERG) machine using a single flash protocol at three light intensities: 10 mcd.s/m
2 , 3000 mcd.s/m2 , 10 000 mcd.s/m2 . Results At 10 mcd.s/m2 the mean b-wave amplitude and implicit time was 102.0 μV (SD ± 41.3 and 95% CI 68.9–135.1) and 78.4 ms (SD ± 8.3 and 95% CI 71.8–85.0). At 3000 mcd.s/m2 the mean a-wave amplitude and implicit time was 69.9 μV (SD ± 20.5 and 95% CI 53.5–86.3) and 17.6 ms (SD ± 1.5 and 95% CI 16.4–18.8) and the mean b-wave amplitude and implicit time was 175.4 μV (SD ± 35.9 and 95% CI 146.7–204.1) and 74.1 ms (SD ± 3.5 and 95% CI 71.2–76.9). At 10 000 mcd.s/m2 the mean a-wave amplitude and implicit time was 89.1 μV (SD ± 27.1 and 95% CI 67.5–110.8) and 16.8 ms (SD ± 1.0 and 95% CI 16.0–17.0) and the mean b-wave amplitude and implicit time was 203.7 μV (SD ± 41.4 and 95% CI 170.6–236.8) and 75.4 ms (SD ± 3.3 and 95% CI 72.8–78.1). Conclusion Electroretinography outside of the typical clinical setting is feasible using a portable ERG system and allows for quick analysis of retinal function in exotic species. [ABSTRACT FROM AUTHOR]- Published
- 2010
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7. The effects of medetomidine hydrochloride on the electroretinogram of normal dogs.
- Author
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Norman, Joanna C., Narfström, Kristina, and Barrett, Paul M.
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DOG diseases , *EYE diseases , *ANIMAL sedation , *VETERINARY medicine , *INTRAVENOUS therapy , *PHOTORECEPTORS , *DISEASES - Abstract
Purpose To determine the effects of a standardized intravenous dose of an α-2 agonist (Domitor®, Orion Pharma, distributed by Pfizer Animal Health, Exton, PA) on the electroretinogram (ERG) response in normal dogs. Methods Twenty-five normal dogs were used to collect ERG responses including a- and b-wave implicit times (IT) and amplitudes (AMP) before and after administration of medetomidine. Dogs were dark adapted for 20 min and ERGs were obtained using the HMsERG (RetVetCorp Inc., Columbia, MO). The QuickRetCheck protocol (Narfström) was employed to provide the following flash intensities: 10 mcd s/m2, 3 cd s/m2, and 10 cd s/m2. ERGs were repeated after 375 µg/m2 of medetomidine intravenously. Statistical analysis of the difference between the responses before and after medetomidine at all flash intensities was performed using a mixed effects model foranova. Results The P value for the effect of medetomidine on each of the ERG responses was < 0.01. The estimates of the effect of medetomidine were (+)1.35 ms, (–)23 µV, (+)3.16 ms, and (–)47 µV for the a-wave IT, a-wave AMP, b-wave IT, and the b-wave AMP, respectively. Conclusions Medetomidine significantly prolongs the implicit time and lowers the amplitude response of both the a- and b-waves in normal dogs at all flash intensities examined. Clinically, however, medetomidine only minimally affects the retinal responses and is a viable choice for use in dog ERGs. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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8. Optimal discrimination of an Abyssinian cat recessive retinal degeneration: a short electroretinogram protocol is more efficient than a long one.
- Author
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Vaegan and Narfström, Kristina
- Subjects
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RETINAL degeneration , *RETINAL diseases , *ELECTRORETINOGRAPHY , *EYE examination , *OPHTHALMOLOGY , *EYE diseases - Abstract
Aim: To determine the diagnostic efficiency of scores in a long protocol electroretinogram (ERG) for Abyssinian cat slow recessive rod/cone dystrophy. Methods: Kittens (n = 22) were bred from homozygous, affected and heterozygous normals. Ophthalmoscopy was regularly performed and disease signs noted. Cats (age ≥ 8 months, 40 sessions, 1-3 repeats) were dark-adapted overnight, anaesthetized and simultaneous binocular ERG recorded using a long protocol. Conventional a- and b-amplitudes and peak implicit times were measured and b/a ratios calculated, initially only for ERG to the maximum photopic and scotopic stimulus Principal components factor analysis was applied to various subsets of these scores plus age attesting. Results: Six cats with ophthalmoscopic change were classed as affected. Three cats, one tested three times, were suspect. The rest were considered normal. The first analysis, of 80 eyes and 37 parameters, showed that the first factor was the only effective one. Using it, the groups overlapped 5%, scotopic amplitudes and b/a ratios loaded higher than peak times, the eyes were very similar, and age and photopic b/a ratios loaded poorly. The groups were discriminable with all the data. A second analysis, with eyes averaged and the 20 measures loading over 0.5 on the first factor, showed better group separation on factor I alone. An iterative search with varying data sets found that factor I was optimal, with eight ERG measures to the three brightest scotopic and one brightest photopic response. It produced a large absolute separation and classified the suspects consistently. With b/a ratios on these four ERG also included, 12 parameters gave better separation. Conclusion: Twelve scores on four ERG separate affected from normal cats with a wide gap and consistently classify suspects. It may work for earlier ages. Additional data probably adds noise. This combination of optimal scores needs confirmation in new data [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
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