3 results on '"Uttayarat, Pimpon"'
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2. Micropatterning of three-dimensional electrospun polyurethane vascular grafts.
- Author
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Uttayarat, Pimpon, Perets, Anat, Li, Mengyan, Pimton, Pimchanok, Stachelek, Stanley J., Alferiev, Ivan, Composto, Russell J., Levy, Robert J., and Lelkes, Peter I.
- Subjects
ELECTROSPINNING ,POLYURETHANES ,VASCULAR grafts ,ENDOTHELIUM ,FIBERS ,TISSUE engineering - Abstract
Abstract: The uniform alignment of endothelial cells inside small-diameter synthetic grafts can be directed by surface topographies such as microgrooves and microfibers to recapitulate the flow-induced elongation and alignment of natural endothelium. These surface micropatterns may also promote directional migration and potentially improve anastomotic ingrowth of endothelial cells inside the synthetic grafts. In this paper, we developed electrospinning and spin casting techniques to pattern the luminal surface of small-diameter polyurethane (PU) grafts with microfibers and microgrooves, respectively, and evaluated endothelial cell orientation on these surface micropatterns. Tracks of circumferentially oriented microfibers were generated by electrospinning PU onto a mandrel rotated at high velocity, whereas longitudinal tracks of microgrooves were generated by spin casting PU over a rotating poly(dimethylsiloxane) mold. We found that both PU grafts possessed longitudinal Young’s moduli in the range of 0.43±0.04 to 2.00±0.40MPa, comparable with values obtained from native artery. Endothelial cells seeded onto the grafts formed confluent monolayers with individual cells exhibiting elongated morphology parallel to the micropatterns. The cells were phenotypically similar to natural endothelium as assessed by the expression of the endothelial cell-specific marker, vascular endothelial cell cadherin. In addition, the cells were also responsive to stimulation with the pro-inflammatory cytokine tumor necrosis factor-α as assessed by the inducible expression of intercellular adhesion molecule-1. These results demonstrate that our micropatterned PU grafts possessed longitudinal Young’s moduli in the same range as native vascular tissue and were capable of promoting the formation of aligned and cytokine-responsive endothelial monolayers. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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3. Diverse Biomedical Applications of Electrospun Scaffolds.
- Author
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Har-el, Yah-el, Frohberg, Michael E., Senel Ayaz, H. Gozde, Perets, Anat, Uttayarat, Pimpon, Gerstenhaber, Jonathan, and Lelkes, Peter I.
- Abstract
In this presentation we will discuss strengths and weaknesses of electrospinning as a platform technology for a wide variety of biomedical applications, such as wound dressings, and vascular grafts as well as for creating complex 3-dimensional (3D) structures for tissue engineering. Based on the choice of natural or synthetic biopolymeric materials used and in conjunction with nano/micro-sized additives and crosslinkers, such as hydroxyapatite or genipin, complex electrospun structures can be tailored to emulate mechanical properties of various organs, ranging from neural tissue to bone. Examples for successfully electrospun biopolymers alone or in combinations with synthetic biomaterials, such as PLGA, include collagen, elastin and chitosan. Our laboratory has recently described the use of chitosan scaffolds for repairing critical-size defects in the cranial bone of mice with and without pre-seeding the scaffolds with mesenchymal stem cells1. Importantly, following mineralization and cross-linking, electrospun chitosan scaffolds emulated the mechanical properties of calvarial bone and were able to induce bone regeneration, even in the absence of stem cell seeding prior to implantation. As a weakness, the tight structure and small pore diameters of electrospun scaffolds usually obstruct cell penetration. We will discuss methods to overcome this shortcoming, e.g., by increasing the pore size using sacrificial polymers and or by creating composite blends with increased elasticity. As an alternative to using animal-derived biomaterials, we will describe recent studies utilizing plant derived compounds, such as soy protein, for generating electrospun bioactive wound dressings that are capable of improving the healing of full-thickness cutaneous wounds in rodent and porcine models2.3. Finally, we will discuss the use of synthetic elastomeric biomaterials, such as polyurethanes, in conjunction with innovative manufacturing technologies for electrospinning complex micropatterned vascular grafts4 that mimic the compliance of natural blood vessels or for creating anisotropic, textile- templated, scaffolds, that might be useful for cardiac repair5. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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