Background: Learning trajectories (LTs) in early mathematics curriculum and teaching have received increasing attention (Baroody et al., 2019; Clements, 2007; Clements & Sarama, 2021; Maloney et al., 2014; Sarama & Clements, 2009). For example, LTs were a core construct in the National Research Council (2009) report on early mathematics education (subtitled "Paths toward excellence and equity") and the notion of levels of thinking was a key first step in the writing of the Common Core State Standards--Mathematics (NGA/CCSSO, 2010). However, little research has directly tested the specific contributions of LTs to teaching compared to instruction provided without LTs (Frye et al., 2013). Objective: The goal of the study was to compare the learning of kindergarteners who received instruction on length measurement following an empirically-validated LT (see Table 1) to those who received an equal amount of time on the same instructional activities that were not sequenced along the LT's developmental progression. Setting: This study is a randomized control trial that took place in an urban school district in a Mountain West state. Population: The sample was composed of 186 kindergarten students (104 girls) from 16 classrooms across six schools (four public; n = 149). Table 2 presents demographic information for the public schools. Intervention: The intervention was composed of three experimental conditions: the LT group, the reverse-order (REV) group, and control group (BAU). Only the LT and REV group received ten one-on-one instructional sessions. In the LT condition each instructor had access to a set of instructional activities that aligned with each level of the developmental progression and selected activities based on the child's present level of thinking. As children demonstrated higher levels of thinking, they were encouraged to use more sophisticated strategies (e.g., Level 3, iterating with a single unit instead of Level 2, using multiple units; Table 1). The REV group received 10 length activities selected from each level of the developmental progression in reverse order (Table 1 and LearningTrajetories.org). Thus, students were exposed to similar activities as the LT condition, but began with the most sophisticated level: Level 5. REV instructors provided feedback about the correctness of children's solutions, but did not modify activities to accommodate less sophisticated levels of thinking. Research Design: We used a randomized control trial. All students for whom we acquired parental consent were administered a pre-assessment prior to random assignment. The pre- and post-assessment was composed of 26 items adapted from extant instruments (Battista, 2012; Clements et al., 2008/2021). We tested two child-level covariates: child sex (0 = girl) and school type (public/private; 0 = public) are coded as binary. Data Collection: and Analysis The research question was examined within a Bayesian hierarchical linear modeling (HLM) framework using the brms package (Bürkner, 2018) in R 3.6.2 (R Core Team, 2019). Bayesian models more accurately quantify and propagate uncertainty (e.g., Kruschke, 2014) and can be more reliable in cases where traditional HLM methods typically fail (Eager & Roy, 2017). Results: Students in the LT and REV condition outperformed their peers in the BAU condition. Contrasts further reveal that students in the LT group also outperform their REV peers = 0.32 (0.57, 0.07). Credible Intervals of 95% were estimated for child gender and whether the child attended a public or private school. However, these intervals include zero and therefore deemed to be statistically non-significant. Discussion: As one of a set of experiments rigorously testing the efficacy of the educational application of learning trajectories (LTs), this study focused on the second assumption of an LT approach: there is a sequence of learning and teaching that is determined by a research-based developmental progression. The topic of length was selected as being both important to early mathematics learning and amenable to the LT and counterfactual conditions. The first of these, intended within the research context to represent the traditional "theme" approach for choosing activities (Helm & Katz, 2016; Katz & Chard, 2000; Tullis, 2011), reversed the sequence of the LT activities to directly test the assumption (REV). The second counterfactual, business-as-usual (BAU), served as a passive control. Students in the LT group outperformed their BAU and their REV peers. The latter contrast, especially, supports the hypothesis that following the developmental progression of an empirically-validated LT promotes learning more than the same activities not in that order. Students in the REV condition also outperformed their peers in the BAU condition. This indicates that the activities, even when implemented in an order other that of the LT's developmental progression, are still effective. This result is similar to that of the studies testing the first LT assumption, those that had a "teach-to-target" counterfactual (Authors, 2019, 2020). That is, these and the present study suggest teaching each contiguous level in developmental order of a LT is more efficacious and thus useful than alternatives, but not necessary to facilitate learning in all cases. However, note that in previous studies using a teach-to-target approach to test assumption 1 (Authors, 2019, 2020) instruction was at levels n + 2 or n + 3, avoiding instruction at n + 1. In the present study, children in the REV condition experienced activities at each level considered in this study (Table 2) and thus the activities eventually crossed over the child's present level of thinking (including n - 1, n, n + 1, etc.). This supports the LT assumption that each builds hierarchically on the concepts and processes of the previous levels (e.g., Goodson, 1982; Sarama & Clements, 2009; van Hiele, 1986). That is, each level is characterized by specific concepts (e.g., mental objects) and processes (mental "actions-on-objects") (Clements et al., 2004; Steffe & Cobb, 1988) that underlie mathematical thinking at level n and serve as a foundation to support successful learning of subsequent levels (Sarama & Clements, 2009). However, the learning process is not intermittent and step-like, but rather incremental and gradually integrative. A critical mass of ideas from each level must be constructed before thinking characteristic of the subsequent level becomes ascendant in the child's thinking and behavior (Clements et al., 2001).