Search

Lycium ruthenicum Murr. is one of the most important shrubs grown in northwest China. Healthy buds and leaves of L. ruthenicum Murr. were selected for the present study. Flower development was divided into six stages, namely, flower bud pre-differentiation (I), late flower differentiation (II), squaring stage (III), dew crown period (IV), open stage (V) and senescent stage (VI). Endogenous hormone content and specific value, soluble sugar, sucrose, starch, and soluble protein were measured, and ABA/IAA, ABA/GA3, ZR/IAA, ZR/GA3, and C/N were calculated in buds and leaves at stage VI. The results showed that ABA, GA3, and ZR content of buds significantly increased from flower bud pre-differentiation to late flower differentiation stage. However, ABA, GA3, and ZR content of leaves had the opposite change trend. From open stage to senescent stage, IAA, ABA, and GA3 content of buds and leaves significantly increased in L. ruthenicum Murr. However, ZR content of buds and leaves significantly decreased from open stage to senescent stage. ABA/IAA, ABA/GA3, ZR/IAA, and ZR/GA3 values of buds significantly increased from lower bud pre-differentiation to late flower differentiation stage. However, ABA/IAA, ABA/GA3, ZR/IAA, and ZR/GA3 values of leaves significantly decreased from lower bud pre-differentiation to late flower differentiation stage. ABA/IAA and ABA/GA3 of buds significantly increased from open stage to senescent stage, but ZR/IAA and ZR/GA3 of buds significantly decreased from open to senescent. At this stage, ABA/IAA, ABA/GA3, ZR/IAA, and ZR/GA3 significantly decreased in L. ruthenicum Murr. The higher soluble sugar and sucrose content in the buds and leaves were beneficial to the flower bud differentiation of L. ruthenicum. The increasing of soluble sugar improved the energy basis to florescence and senescent. The carbohydrates metabolism enhanced from open stage to senescent stage and nitrogen metabolism reduced from open stage to senescent stage of L. ruthenicum. [ABSTRACT FROM AUTHOR]

[Objectives] This study aims at exploring the effects of growth regulators on floral differentiation, expression of flowering related genes and nutrient accumulation of pear hybirds. [ Methods] This study took 'Mantianhong' x 'Dangshansuli' progenies as materials, and all the individuals were five years old but still have no flower. We sprayed the 6-BA and ethephon( Eth) (100 mg·kg-1 6-BA, 100 mg · kg-1 Eth, 100mg ·kg-1 6-BA+1 00 mg · kg-1 Eth) with water control during the period of flower bud differentiation. [Results] All different growth regulator treatments can facilitate the florescence of the progenies. The mixed 6-BA and ethephon treatment increased the most flower buds among three treatments. All treatments promoted the expression of FLOWERING LOCUS T( FT) and LEAFY(LFY) genes during floral bud differentiation, and inhibited the expression of TERMINAL FLOWER 1(TFL1) gene; the treatments enhanced the content of ZT and ABA, reduced the hormone content of GA3 and IAA, and improved the content of starch and the ratio of C/N in floral buds. [Conclusions] The synthesis analysis revealed that gene expression, endogenous hormone and nutriment influenced each other, and they regulated floral initiation of hybrid progenies together. [ABSTRACT FROM AUTHOR]