1. Developmental exposure to perfluorooctane sulfonate(PFOS) impairs the endometrial receptivity.
- Author
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Ren R, Zhou X, Jia T, Wang B, Liu A, Gao M, Song J, Wang L, Jing Y, Yu L, Shen H, and Zhang X
- Subjects
- Female, Humans, Animals, Mice, Apoptosis drug effects, Mesenchymal Stem Cells drug effects, Mesenchymal Stem Cells metabolism, Cell Differentiation drug effects, Stromal Cells drug effects, Stromal Cells metabolism, Embryo Implantation drug effects, Mitochondria drug effects, Mitochondria metabolism, Fluorocarbons toxicity, Alkanesulfonic Acids toxicity, Endometrium drug effects, Endometrium metabolism
- Abstract
Perfluorooctane sulfonate (PFOS) is a widely used chemical in industrial production. It can be introduced into the environment through multiple pathways and exhibits resistance to degradation. Recent research has demonstrated a significant correlation between its exposure levels in the human body and the incidence of various diseases. The expression of genes related to endometrial receptivity and the differentiation of human endometrial stromal cells (hESCs) were assessed in this study concerning PFOS. In this study, we investigated the effect of PFOS exposure on endometrial tolerance by cell and animal experiments. The activity against endometrial mesenchymal cells was significantly reduced by PFOS intervention, and the apoptosis flow assay results showed that PFOS significantly promoted cell death in a concentration-dependent manner. Transmission electron microscopy results revealed mitochondrial damage in the PFOS-intervened group, and WB results showed that the expression levels of endometrial tolerance-related proteins Homeobox A10 (HOXA10) and integrin beta3 (ITGB3) were decreased, and the expression level of Forkhead box O1 (FOXO1) protein was increased. Animal studies have shown that PFOS exposure can change uterine morphology, cause obvious damage to pinopodes morphology, change the estrous cycle of mice, and affect endometrial receptivity In the present study, we found that PFOS may synergistically affect the viability of endometrial mesenchymal stromal cells through accumulation in vivo, and that PFOS may contribute to the failure of embryo implantation by affecting mitochondrial function and consequently endometrial permissive sites., Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Ethics approval and consent to participate: Endometrial tissue sample collection for this study was approved by the Ethics Committee of the First Hospital of Lanzhou University (Approval number: LDYYSZLLKH2023-04). Endometrial tissue samples were obtained from women attending the Reproductive Center of the First Hospital of Lanzhou University. Written informed consent was obtained from patient prior to acquisition of samples in accordance with the Declaration of Helsinki. Animal experimentation protocols were approved by the Ethics Committee of the First Hospital of Lanzhou University (Approval number: LDYYSZLLKH2023-05), and were conducted in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals and ARRIVE guidelines. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2025. The Author(s).)
- Published
- 2025
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