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Excessive accumulation of reducing agents in the ER leads to a constitutively high UPR. And the co-function of GSH, Cys and HOCl in biological processes is not well understood. To address this, a TP probe, NPCC, was developed for monitoring reductive stress in the ER. It can also distinguish cancer cells from normal cells.

• A new two-photon fluorescent probe for HNO was developed. • The probe showed faster response time (2 min), lower detection limit (32 nm) and strong anti-interference ability. • The probe can target the ER and visualization of NO and H 2 S cross-talk on the ER for the first time. Nitroxyl (HNO) is a one-electron reduction product of nitric oxide (NO), which mainly exists in protonated form during physiological processes. As a kind of reactive nitrogen species (RNS), HNO has significant correlations with many physiological and pathological pathways. In this study, we report a two-photon fluorescent probe R2, which can detect HNO based on the reaction with a triarylphosphine group. Upon exposure to HNO, the probe R2 holds "naked eye" recognition, high selectivity and fast response (2 min). These excellent spectral properties promoted the detection of exogenous and endogenous HNO in MCF-7 cells. Furthermore, the probe could be selectively located in the endoplasmic reticulum (ER) with high biocompatibility and successfully apply to visualize NO/H 2 S cross-talk to generate HNO. These results support that R2 will provide a powerful tool to elucidate the physiological and pathological functions of HNO in the ER. [ABSTRACT FROM AUTHOR]

A ER-targetable probe R1 for HOCl was synthesized. R1 shows excellent selectivity and high sensitivity, excellent anti-interference, and can quickly reach the response platform. • A new ER-targetable ICT-based fluorescent probe R1 for HClO was developed. • Probe have rapid response (30 s), big Stock's shift and a wide range of pH(7–13). • The imaging in EC1 cells demonstrated its value of practical application. The endoplasmic reticulum is the largest organelle in the cell. Studies have shown that the concentration of HClO is related to many diseases. A new type of probe R1 has positioned to the endoplasmic reticulum. The probe shows good positioning effect, excellent selectivity and high sensitivity, excellent anti-interference, and can quickly reach the response platform. The obvious color change can be used for naked eye observation. Due to its low cytotoxicity, probe R1 was successfully used for EC1 cells imaging under a laser scanning confocal microscope. [ABSTRACT FROM AUTHOR]