1. Reductive stress imaging in the endoplasmic reticulum by using living cells and zebrafish.
- Author
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Niu H, Zhang Y, Zhao F, Mo S, Cao W, Ye Y, and Zhao Y
- Subjects
- Animals, Coumarins chemical synthesis, Cysteine chemistry, Cysteine metabolism, Fluorescent Dyes chemical synthesis, Glutathione chemistry, Glutathione metabolism, Goats, HeLa Cells, Humans, Hypochlorous Acid chemistry, Hypochlorous Acid metabolism, Mice, Microscopy, Confocal methods, Microscopy, Fluorescence methods, Oxidation-Reduction, Pyrazoles chemical synthesis, Zebrafish, Coumarins chemistry, Endoplasmic Reticulum metabolism, Endoplasmic Reticulum Stress physiology, Fluorescent Dyes chemistry, Pyrazoles chemistry
- Abstract
Excessive accumulation of reducing agents in the ER leads to a constitutively high UPR. And the co-function of GSH, Cys and HOCl in biological processes is not well understood. To address this, a TP probe, NPCC, was developed for monitoring reductive stress in the ER. It can also distinguish cancer cells from normal cells.
- Published
- 2019
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