1. Flotillins promote T cell receptor sorting through a fast Rab5-Rab11 endocytic recycling axis.
- Author
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Redpath GMI, Ecker M, Kapoor-Kaushik N, Vartoukian H, Carnell M, Kempe D, Biro M, Ariotti N, and Rossy J
- Subjects
- Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Humans, Jurkat Cells, Membrane Proteins, Microscopy, Confocal, Protein Transport, Receptors, Antigen, T-Cell genetics, Cell Membrane metabolism, Endocytosis, Endosomes metabolism, Receptors, Antigen, T-Cell metabolism, rab GTP-Binding Proteins metabolism, rab5 GTP-Binding Proteins metabolism
- Abstract
The targeted endocytic recycling of the T cell receptor (TCR) to the immunological synapse is essential for T cell activation. Despite this, the mechanisms that underlie the sorting of internalised receptors into recycling endosomes remain poorly understood. To build a comprehensive picture of TCR recycling during T cell activation, we developed a suite of new imaging and quantification tools centred on photoactivation of fluorescent proteins. We show that the membrane-organising proteins, flotillin-1 and -2, are required for TCR to reach Rab5-positive endosomes immediately after endocytosis and for transfer from Rab5- to Rab11a-positive compartments. We further observe that after sorting into in Rab11a-positive vesicles, TCR recycles to the plasma membrane independent of flotillin expression. Our data suggest a mechanism whereby flotillins delineate a fast Rab5-Rab11a endocytic recycling axis and functionally contribute to regulate the spatial organisation of these endosomes.
- Published
- 2019
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