1. Interfacing neurons on carbon nanotubes covered with diamond
- Author
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Silke Seyock, Lionel Rousseau, Clément Hébert, Emmanuel Scorsone, Andreas Offenhäusser, Philippe Bergonzo, Vanessa Maybeck, Gaelle Lissorgues, Institute of Complex Systems (ICS), Forschungszentrum Jülich GmbH | Centre de recherche de Juliers, Helmholtz-Gemeinschaft = Helmholtz Association-Helmholtz-Gemeinschaft = Helmholtz Association, Laboratoire Capteurs Diamant (LCD-LIST), Département Métrologie Instrumentation & Information (DM2I), Laboratoire d'Intégration des Systèmes et des Technologies (LIST (CEA)), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Laboratoire d'Intégration des Systèmes et des Technologies (LIST (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Electronique, Systèmes de communication et Microsystèmes (ESYCOM), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Paris-Est Marne-la-Vallée (UPEM)-ESIEE Paris, Universitat Autònoma de Barcelona (UAB), European Project: 280433,EC:FP7:NMP,FP7-NMP-2011-SMALL-5,NEUROCARE(2012), Laboratoire d'Intégration des Systèmes et des Technologies (LIST), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Laboratoire d'Intégration des Systèmes et des Technologies (LIST), and Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Paris-Est Marne-la-Vallée (UPEM)-ESIEE Paris
- Subjects
NANOCRYSTALLINE DIAMOND ,Cell viability ,Scanning electron microscope ,NANOFIBER ,General Chemical Engineering ,Nanotechnology ,02 engineering and technology ,Carbon nanotube ,Substrate (electronics) ,engineering.material ,010402 general chemistry ,01 natural sciences ,Focused ion beam ,BIOCOMPATIBILITY ,law.invention ,CELL-ADHESION PROPERTIES ,[SPI.MAT]Engineering Sciences [physics]/Materials ,Cell attachments ,Electrode surfaces ,CULTURE ,law ,Nano-crystalline diamonds ,BORON-DOPED DIAMOND ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials ,Single structure ,Bioelectronics ,TRANSMISSION ELECTRON-MICROSCOPY ,NANOWIRE ARRAYS ,Chemistry ,SURFACES ,Diamond ,FUNCTIONALIZED DIAMOND ,General Chemistry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,[SPI.TRON]Engineering Sciences [physics]/Electronics ,[SPI.ELEC]Engineering Sciences [physics]/Electromagnetism ,Transmission electron microscopy ,Chemical window ,ddc:540 ,Electrode ,engineering ,Different heights ,Neuronal cell ,0210 nano-technology - Abstract
International audience; A recently discovered material, carbon nanotubes covered with diamond (DCNTs) was tested for its suitability in bioelectronics applications. Diamond shows advantages for bioelectronics applications (wide electro chemical window and bioinertness). This study investigates the effect of electrode surface shape (flat or three dimensional) on cell growth and behavior. For comparison, flat nanocrystalline diamond substrates were used. Primary embryonic neurons were grown on top of the structures and neither incorporated the structures nor did they grow in between the single structures. The interface was closely examined using focused ion beam (FIB) and scanning electron microscopy. Of special interest was the interface between cell and substrate. 5% to 25% of the cell membrane adhered to the substrate, which fits the theoretical estimated value. While investigating the conformity of the neurons, it could be observed that the cell membrane attaches to different heights of the tips of the 3D structure. However, the aspect ratio of the structures had no effect on the cell viability. These results let us assume that not more than 25% of cell attachment is needed for the survival of a functional neuronal cell.
- Published
- 2017
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