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The growing prevalence of foodborne zoonotic infections increases the risk of emergence of microorganisms with acquired resistance to antimicrobial drugs. Such microorganisms are capable of horizontal transmission of R-plasmids of antibiotic resistance to other types of bacteria. The results of our monitoring in 2021, in accordance with the State Strategy of Ukraine to curb the development of antimicrobial resistance and reduce the risks of the formation and spread of antimicrobial-resistant strains of microorganisms, showed high resistance of enterobacteria, enterococci and Campylobacter isolated from pigs to antibiotics of various groups. Among the isolated cultures of Esherichia coli, the ability of some strains to produce extended-spectrum β-lactamases (ESBL) was found. Testing of the experimental isolates for antibiotic resistance and screening for possible production of acquired resistance enzymes was carried out by the disc diffusion method with antibiotic disks of different groups. Out of 542 samples of appendix from pigs from farms in different regions of Ukraine, 138 isolates were isolated and identified. Among them: Esherichia coli – 68 strains; Enterococcus faecalis – 57; Enterococcus faecium – 9; Salmonella spp. – 2 and Campylobacter spp. – 2. Antibiotic resistance testing showed that 10 (7.3% of the isolates) of different bacterial species remained susceptibile to all antibiotics used. The remaining 128 test isolates (93.5% of the isolates) showed antibiotic resistance. The largest number of antibiotic-resistant commensal and zoonotic bacteria was detected in pig farms of Donetsk, Kyiv, Dnipro, Kirovohrad and Chernihiv regions. Escherichia coli and Enterococcus spp. were most often isolated from pigs; in smaller quantities Salmonella spp. and Campylobacter spp. All strains of Salmonella spp. faecium showed resistance to 1 to 3 antibiotics of different groups. Among the antibiotic-resistant E. faecalis, 1 strain with resistance to vancomycin was detected. Polyantibiotic resistance was inherent in E. colistrains. Among the polyantibiotic-resistant E. colistrains, 4 strains were found and confirmed to have acquired resistance due to the production of ESBL. [ABSTRACT FROM AUTHOR]

Complex probiotic preparations with spore-forming microorganisms of the genus Bacillus and other types of bacteria in their composition have a high biological activity and are of scientific and practical interest. In our laboratory experiments, we determined the level of antagonistic activity of the newly developed probiotic preparation containing strains of bacteria B.subtilis, B. licheniformis, B. coagulans in a complex with Enterococcus faecium, products of their own fermentation, other natural substances and a sorbent made of natural aluminosilicates. The antagonistic properties of the probiotic preparation were examined by diffusion methods of delayed antagonism and agar blocks. In order to identify the antagonistic action of the experimental probiotic preparation, we used Gram-negative and Grampositive test cultures of bacteria from the Museum of Strains of Microorganism Cultures of the Research Bacteriology Department. The results of laboratory studies indicated the antagonistic effectiveness of the experimental probiotic preparation in its interaction with test microorganisms. This was confirmed by the indicators of the diameters of the growth inhibition zones of the tested bacteria, ranging 27– 36 and more and corresponding to high and very high levels of antagonistic activity of the experimental probiotic. The use of the developed probiotic preparation in poultry farming would ensure the correct formation of microbiocenosis in gthe astrointestinal tract of poultry from hatching, increase local immunity, support a stable epizootic situation by preventing bacterial infections among poultry through biological means, and therefore contribute to production of ecologically high-quality and safe products of poultry farming. [ABSTRACT FROM AUTHOR]