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Benthic biofilms are often assumed to control terrestrially-derived dissolved organic carbon (tDOC) uptake in streams. We tested this by comparing 13C-enriched ryegrass leachate uptake in an agricultural and a forest stream, hypothesizing that a greater abundance of autotrophic biofilms in the agricultural stream would cause its whole-stream tDOC uptake to be comparatively low. We measured whole-stream and biofilm tDOC tracer uptake, metabolism, bacterial and algal diversity, and nutrient status of benthic epilithic biofilms, and assessed whole-stream hydromorphology. Whole-stream uptake of tDOC was six times lower in the agricultural (3.0 mg m−2 day−1) than in the forest (19.0 mg m−2 day−1) stream, and tDOC uptake velocity indicated lower tDOC demand in the agricultural (1.2 mm min−1) than in the forest (1.9 mm min−1) stream. The agricultural stream differed from the forest stream by slightly lower transient storage capacity and higher benthic biofilm bacterial abundance and production, lower biofilm biomass and lower biofilm molar C:N, C:P, and N:P ratios. Changes in epilithic biofilms contributed little to the differences in whole-stream tDOC tracer uptake between streams, as biofilm tDOC uptake only amounted to 4% and 13% of whole-stream uptake in the forest and agricultural stream, respectively. This comparison of a forest and an agricultural stream suggests that agricultural stressors have the potential to diminish both whole-stream tDOC uptake and uptake efficiency. Furthermore, the weak link between biofilm and whole-stream tDOC uptake implies that benthic biofilms characteristics are poor predictors for human impacts on tDOC uptake in agricultural streams and that hot spots of tDOC uptake are likely situated in the hyporheic zone or in the stream water column.

Dissolved oxygen (DO) in the hyporheic zone (HZ) is a crucial parameter for the survival of many stream organisms and is involved in a multitude of aerobic chemical reactions. However, HZ DO budgets are easily perturbed by climate change and anthropogenic processes that have caused increased deposition of fine sediments (< 2 mm) in many stream beds. The fine sediment fraction hampers exchange of DO-rich stream water with the HZ. In this study we performed a raster sampling approach (0.90 cm length x 1.50 cm width; 30 cm distance between sampling points) at sediment depths of 10 and 25 cm with a focus on DO and its stable isotopes (δ18ODO). The aim was to analyze small-scale turnover patterns in a forested (site 1) and an anthropogenically influenced stream section (site 2) in a 3rd order stream in southern Germany. Grain size analyses showed similar average fine sediment fractions at site 1 (42.5 ±13.7 %) and site 2 (46.3 ±10.8 %). They increased with depth at both sites (38.5 ± 6.3 %, 0-15 cm; 46.5 ± 17.4 %, 15-30 cm at site 1 and 40.6 ±4.5 %, 0-15 cm; 52.0 ±12.2 %, 15-30 cm at site 2). DO concentrations in the HZ ranged from 1.4 to 4.5 mg L-1 (2.0 ±0.7 mg L-1) and 1.5 to 1.8 mg L-1 (1.7 ±0.1 mg L-1) at site 1 and from 1.2 to 2.9 mg L-1 (1.6 ±0.5) and 1.0 to 2.4 mg L-1 (1.6 ±0.4) at site 2 at 10 and 25 cm depth, respectively. The low DO concentrations in the HZ suggest high DO consumption rates and reduced exchange with stream water. This is possibly a result of increased fine sediment proportions. However, other factors such as organic carbon contents and increased respiration rates may also influence DO gradients. In contrast, the stream water had an average DO concentration of 9.8 ±0.2 mg L-1. Associated δ18ODO values of the open water (23.4 ±0.1 ‰) differed from those of sediment waters that showed averages of +22.5 ±0.5 ‰ and +22.4 ±0.3 ‰ at site 1 and +22.5 ±0.4 ‰ and +22.3 ±0.2 ‰ at site 2 at 10 and 25 cm depth, respectively. These sedimentary values indicated dominant photosynthesis, even though due to absence of light in the subsurface this process seems unlikely. Therefore, kinetically-driven processes such as diffusion, interactions with Fe or unknown DO sources within the HZ might have caused such 16O-enriched values. Our findings suggest that the analyses of DO, δ18ODO and fine sediment gradients in the HZ should be combined with stable carbon isotope measurements to further our understanding of hyporheic processes relevant for stream biota.

The science of the total environment 769, 144324 (2021). doi:10.1016/j.scitotenv.2020.144324, Published by Elsevier Science, Amsterdam [u.a.]

The assessment of the exposure of aquatic wildlife to complex environmental mixtures of chemicals originating from both point and diffuse sources and evaluating the potential impact thereof constitutes a significant step towards mitigating toxic pressure and the improvement of ecological status. In the current proof-of-concept study, we demonstrate the potential of a novel Aggregated Biomarker Response (ABR) approach involving a comprehensive set of biomarkers to identify complex exposure and impacts on wild brown trout (Salmo trutta fario). Our scenario used a small lowland river in Germany (Holtemme river in the Elbe river catchment) impacted by two wastewater treatment plants (WWTP) and diffuse agricultural runoff as a case study. The trout were collected along a pollution gradient (characterised in a parallel study) in the river. Compared to fish from the reference site upstream of the first WWTP, the trout collected downstream of the WWTPs showed a significant increase in micronucleus formation, phase I and II enzyme activities, and oxidative stress parameters in agreement with increasing exposure to various chemicals. By integrating single biomarker responses into an aggregated biomarker response, the two WWTPs' contribution to the observed toxicity could be clearly differentiated. The ABR results were supported by chemical analyses and whole transcriptome data, which revealed alterations of steroid biosynthesis and associated pathways, including an anti-androgenic effect, as some of the key drivers of the observed toxicity. Overall, this combined approach of in situ biomarker responses complemented with molecular pathway analysis allowed for a comprehensive ecotoxicological assessment of fish along the river. This study provides evidence for specific hazard potentials caused by mixtures of agricultural and WWTP derived chemicals at sublethal concentrations. Using aggregated biomarker responses combined with chemical analyses enabled an evidence-based ranking of sites with different degrees of pollution according to toxic stress and observed effects.

Emerging pollutants are ubiquitous in the aquatic system and may pose risks to aquatic ecosystems. The quantification and prediction of environmental partitioning of these chemicals in aquatic systems between water, sediment and biota is an important step in the comprehensive assessment of their sources and final fates in the environment. In this multi-compartment field study, we applied equilibrium partitioning theory and chemical activity estimates to investigate the predictability of concentrations in Gammarus pulex as a model invertebrate from water and sediment in a typical small central European river. Furthermore, K OW -based and LSER approaches were assessed for the calculation of sediment organic carbon-, lipid-, and protein-water partitioning coefficients and activity ratios between the different compartments. Gammarid-water activity ratios close to unity have been observed for many chemicals, while sediment-water and sediment-biota chemical activity ratios exceeded unity by up to six orders of magnitudes. Causes may be: disequilibrium due to slow desorption kinetics and/or an underestimation of partition coefficients due to the presence of strongly adsorbing phases in the sediments. Water concentrations, particularly when using LSER for prediction of partition coefficients were good predictors of internal concentrations in gammarids for most emerging pollutants. Some hydrophilic chemicals such as the neonicotinoid imidacloprid tend to accumulate more in G. pulex than expected from equilibrium partitioning. This conclusion holds both for K OW as well as for LSER-based predictions and suggests previously unidentified mechanisms of bio-accumulation which may include binding to specific protein structures.

Agricultural and urban land use has dramatically increased over the last century and one consequence is the release of anthropogenic chemicals into aquatic ecosystems. One of the rarely studied consequences is the effect of land use change on internal concentrations of organic micropollutants (OMPs) in aquatic invertebrates and its effects on their genotype diversity. Here, we applied population genetic and internal concentrations of OMPs analyses to determine evolutionary implications of chemical pollution on Gammarus pulex populations from a natural and two agricultural streams. Along 14 consecutive months sampled, 26 different OMPs were quantified in G. pulex extracts with the highest number, concentration, and toxic pressure in the anthropogenically stressed stream ecosystems. Our results indicate distinct internal OMP profiles and changes in both genetic variation and genetic structure in streams affected by anthropogenic activity. Genetic variation was attributed to chemical pollution whereas changes in the genetic structure were attributed to environmental disturbances, such as changes in discharge in the impacted stream ecosystems, which worked both independently and in tandem. Finally, we conclude that human-impacted streams are subjected to severe alterations in their population genetic patterns compared to nonimpacted stream ecosystems.