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Wastewater discharge is considered to be a significant point source of microplastic (MPs) release into the marine environment. This study is the first attempt to quantify MPs released from the wastewater outfall from Bandar Abbas City into the Persian Gulf. Two wastewater discharge stations at Gursuzan and Suru were sampled. MPs were isolated by an oxidative procedure and subsequent density separation using ZnCl 2 solution. The average MP concentration in wastewater and sludge were 70.66 (±14.12, SD) MP.35 L -1 and 6070 (±807.25) MPs.kg -1 , respectively at Confidence Level (CL) (95.0%). The most commonly recovered polymers were polyethylene (PE) and polypropylene (PP) in all size classes. Our findings provides a baseline of MP concentration in wastewater streams and slurry that is discharged from the Bandar Abbas wastewater treatment facility into the Persian Gulf. This highlights the need to undertake more studies at water treatment plants in the region for a realistic assessment of MP discharge into the Persian Gulf., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Pollution and health risk assessment models were used to study the effects of effluents discharged into the Inyishi River, Ikeduru LGA, Imo State, Nigeria. The study aims at investigating the environmental health effects of wastewater effluent discharge from the aluminum extrusion company on the Inyishi River. Samples were collected from both the aluminum extrusion company and Inyishi River and analyzed for physicochemical and trace metal parameters. Models adopted for assessment include transfer factor (TF), contamination factor (Cf), pollution load index (PLI), ecological risk factor (Er), water quality index (WQI), hazard quotient (HQ), and hazard index (HI). The results of the study revealed that pH and TH were within the Nigerian Standard for Drinking Water Quality (NSDWQ) and World Health Organization (WHO) standards. EC values at 2235 ± 0.001 and 105 ± 0.03 μS/cm for S 3 and S 4 respectively were higher than the permissible standards, while the values of EC at 24.04 ± 0.03, 5.26 ± 0.005, and 0.05 ± 0.01 μS/cm respectively for S 1 , S 2 , and S R are within the WHO standard. Very high concentration of SO 4 2- was recorded at S 3 , while low concentration was measured at S 2 . Mean values of heavy metal ions studied were within NSDWQ and WHO standards for safe drinking water except for Al, Zn, Cd, Fe, and Mn, with the mean values of metal concentrations observed in the order Al > Fe > Zn > Mn > Cd > Cr. Positive linear correlation based on heavy metal contamination was observed for samples S 1 and S 2 with S R . High TF values were observed in decreasing order Mn > Cd > Cr > Fe > Zn. Similarly, very high CF was observed for Cd and Al while the PLI was generally high for all the samples. Low Er values were observed for all metals except for Cd, with the mean value of Er observed in the decreasing order Cd ˃ Mn ˃ Zn ˃ Cr. The river water sample generally revealed high WQI and was thus categorized as poor quality. HQ and HI values were below unity (< 1), but might pose health risk to children in cases of prolonged dermal adsorption. In conclusion, the quality of the Inyishi River has been negatively impacted by the wastewater effluent from the aluminum extrusion company. Proper treatment of the river water is therefore recommended before use for domestic purposes as prolonged usage of the untreated water might likely pose serious environmental health risks.

Sewage treatment plants (STPs) are one of the sources of pathogens discharged into surface water. An investigation was carried out over the duration of 12 months in Henan Province, China, to evaluate the health influence of municipal wastewater effluent discharge on water quality of the receiving water. A discharge-based quantitative microbial risk assessment (QMRA) was employed, taking into account the vegetables consumption habits of the Chinese, population subgroups with different immune statuses and ages, to evaluate the incremental disease burden from agricultural irrigation and swimming exposure scenarios associated with increased concentration of the protozoan Cryptosporidium and/or Giardia in the receiving river. The results shown that all the STP influent samples contained Cryptosporidium and Giardia with average density of 142.31 oocysts/L and 1187.06 cysts/L, respectively. The QMRA results demonstrated that the estimated additional health burdens due to discharged effluent for both parasites were slightly violated the threshold of 10 -6 DALYs per person per year set by WHO. Mitigation measures should be planned and executed by season since more disease burdens were borne during hot season than other seasons. The sensitivity analysis highlighted the great importance of stability of STP treatment process. This study provides useful information to improve the safety of surface water and deduce the disease burden of the protozoa in Henan Province and other region inside and outside China., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

The present work envisages the possible geometry of a contaminated plume of groundwater near hospital facilities by combining GIS (Geographic Information System) and geophysical methods. The rock underlying the soil and thin sedimentary cover of the study area is moderately fractured quartzite, which makes aquifers vulnerable to pollution. The GIS methodology is used to calculate the area that would be affected by the effluent source of residual water, based on algorithms that consider ground surface mapping (slopes, orientations, accumulated costs and cost per distance). Geophysical methods (electromagnetic induction and electric resistivity tomography) use changes in the electrical conductivity or resistivity of the subsurface to determine the geometry of the discharge and the degree of contamination. The model presented would allow a preliminary investigation regarding potential corrective measures., Competing Interests: The authors declare no conflict of interest.

With the intensification of human activities, fresh water resources are increasingly being exposed to contamination from effluent disposal to land. Thus, there is a greater need to identify the sources and pathways of water contamination to enable the development of better mitigation strategies. To track discharges of domestic effluent into soil and groundwater, 10 synthetic double-stranded DNA (dsDNA) 3 tracers were developed in this study. Laboratory column experiment and field groundwater and soil lysimeter studies were carried out spiking DNA with oxidation-pond domestic effluent. The selected DNA tracers were compared with a non-reactive bromide (Br) tracer with respect to their relative mass recoveries, speeds of travel and dispersions using the method of temporal moments. In intact stony soil and gravel aquifer media, the dsDNA tracers typically showed earlier breakthrough and less dispersion than the Br tracer, and underwent mass reduction. This suggests that the dsDNA tracers were predominantly transported through the network of larger pores or preferential flow paths. Effluent tracking experiments in soil and groundwater demonstrated that the dsDNA tracers were readily detectable in effluent-contaminated soil and groundwater using quantitative polymerase chain reaction. DNA tracer spiked in the effluent at quantities of 36μg was detected in groundwater 37m down-gradient at a concentration 3-orders of magnitude above the detection limit. It is anticipated it could be detected at far greater distances. Our findings suggest that synthetic dsDNA tracers are promising for tracking effluent discharges in soils and groundwater but further studies are needed to investigate DNA-effluent interaction and the impact of subsurface environmental conditions on DNA attenuation. With further validation, synthetic dsDNA tracers, especially when multiple DNA tracers are used concurrently, can be an effective new tool to track effluent discharge in soils and groundwater, providing spatial estimation on the presence or absence of contamination sources and pathways., (Copyright © 2017 Elsevier B.V. All rights reserved.)

The release into the environment of antimicrobial compounds from both human and agricultural sources is a growing global concern. The Grand River watershed, the largest mixed-use watershed in southern Ontario, receives runoff from intensive animal production as well as municipal wastewater effluents from a rapidly increasing human population. A survey of surface waters and wastewater effluents was conducted across the watershed to assess the occurrence and distribution of several antimicrobials (i.e., trimethoprim, sulfamethoxazole, sulfamethazine, lincomycin, and monensin) and chemical indicators (i.e., ammonia, nitrate, ibuprofen, venlafaxine, atrazine) and to characterize exposure levels. The human antimicrobials trimethoprim and sulfamethoxazole were detected in the urban areas of the main channel at mean concentrations of 8 ± 7 ng/L and 31 ± 24 ng/L, respectively, but at much lower concentrations in the agricultural tributaries. In contrast, the veterinary antimicrobial sulfamethazine was detected at a mean concentration of 11 ± 9 ng/L in the main channel, and at a much higher concentration in the agricultural tributaries. Lincomycin was detected in only two river samples and not in the effluents while monensin was not detected in all samples. The herbicide atrazine was detected at very low concentrations in the surface waters of both the tributaries and the main channel. The concentrations of the antimicrobials and chemical indicators generally increased downstream of the confluences with agricultural tributaries and effluent outfalls. In the wastewater effluents, the concentrations of trimethoprim, sulfamethoxazole, ibuprofen, and venlafaxine decreased with increasing treatment levels (i.e., secondary to tertiary) as indicated by ammonia/nitrate concentrations. There was a strong correlation among trimethoprim, sulfamethoxazole, and venlafaxine in the main channel and in the wastewater effluents. While the environmental concentrations of antimicrobials in the watershed are low relative to toxicity thresholds, their persistence in the environment may be an important consideration in defining strategies for future water management., (Copyright © 2015 Elsevier B.V. All rights reserved.)

Contaminants released from wastewater treatment plants can persist in surface waters for substantial distances. Much research has gone into evaluating the fate and transport of these contaminants, but this work has often assumed constant flow from wastewater treatment plants. However, effluent discharge commonly varies widely over a 24-hour period, and this variation controls contaminant loading and can profoundly influence interpretations of environmental data. We show that methodologies relying on the normalization of downstream data to conservative elements can give spurious results, and should not be used unless it can be verified that the same parcel of water was sampled. Lagrangian sampling, which in theory samples the same water parcel as it moves downstream (the Lagrangian parcel), links hydrologic and chemical transformation processes so that the in-stream fate of wastewater contaminants can be quantitatively evaluated. However, precise Lagrangian sampling is difficult, and small deviations - such as missing the Lagrangian parcel by less than 1h - can cause large differences in measured concentrations of all dissolved compounds at downstream sites, leading to erroneous conclusions regarding in-stream processes controlling the fate and transport of wastewater contaminants. Therefore, we have developed a method termed "verified Lagrangian" sampling, which can be used to determine if the Lagrangian parcel was actually sampled, and if it was not, a means for correcting the data to reflect the concentrations which would have been obtained had the Lagrangian parcel been sampled. To apply the method, it is necessary to have concentration data for a number of conservative constituents from the upstream, effluent, and downstream sites, along with upstream and effluent concentrations that are constant over the short-term (typically 2-4h). These corrections can subsequently be applied to all data, including non-conservative constituents. Finally, we show how data from other studies can be corrected., (© 2013.)

While marine aquaculture, or mariculture, has been growing rapidly and globally in recent decades, many environmental concerns remain to be fully addressed to achieve its long-term goal of sustainable development. This paper aims to provide a synthesized perspective on these issues by reviewing and discussing the characterization, transport, and current modelling and management tools associated with effluents released from mariculture sites. Specifically, we examined the effluent characteristics and behavior from source-to-sink, including the composition and load of effluent discharge, its transport and transformation processes in the water column and at the seabed, and its impacts on the pelagic and benthic environments. We then focused on management-related issues, including the setting of the regulatory mixing zone, the establishment of environmental standards, monitoring measures, and modelling techniques to depict the current state-of-the-art modes in a global context. Our study shows that while substantial progress has been made in understanding the nature of the mariculture effluent, as well as in monitoring and modelling its transport and fate, the regulatory framework still lags behind in many countries where the mariculture industry is relevant. This is particularly evident in the lack of consistent criteria for the definition of regulatory mixing zones and the associated environmental standards for water quality and benthic impacts. Besides, as new predictive models are emerging quickly, their proper evaluation and validation are imperative in view of their increasing application in regulatory practices. This review is intended to provide references for advancing regulatory management of mariculture effluents, as well as for promoting sustainable mariculture development. [ABSTRACT FROM AUTHOR]