Search

Although it is generally accepted that δ 15 N in lichen reflects predominating N isotope sources in the environment, confirmation of the direct correlation between lichen δ 15 N and atmospheric δ 15 N is still missing, especially under field conditions with most confounding factors controlled. To fill this gap and investigate the response of lichens with different tolerance to atmospheric N deposition, thalli of the sensitive Evernia prunastri and the tolerant Xanthoria parietina were exposed for ten weeks to different forms and doses of N in a field manipulation experiment where confounding factors were minimized. During this period, several parameters, namely total N, δ 15 N and chlorophyll a fluorescence, were measured. Under the experimental conditions, δ 15 N in lichens quantitatively responded to the δ 15 N of released gaseous ammonia (NH 3 ). Although a high correlation between the isotopic signatures in lichen tissue and supplied N was found both in tolerant and sensitive species, chlorophyll a fluorescence indicated that the sensitive species very soon lost its photosynthetic functionality with increasing N availability. The most damaging response to the different N chemical forms was observed with dry deposition of NH 3 , although wet deposition of ammonium ions had a significant observable physiological impact. Conversely, there was no significant effect of nitrate ions on chlorophyll a fluorescence, implying differential sensitivity to dry deposition versus wet deposition and to ammonium versus nitrate in wet deposition. Evernia prunastri was most sensitive to NH 3 , then NH 4 + , with lowest sensitivity to NO 3 - . Moreover, these results confirm that lichen δ 15 N can be used to indicate the δ 15 N of atmospheric ammonia, providing a suitable tool for the interpretation of the spatial distribution of NH 3 sources in relation to their δ 15 N signal., (Copyright © 2018. Published by Elsevier B.V.)

Reactive nitrogen (Nr) is an important driver of global change, causing alterations in ecosystem biodiversity and functionality. Environmental assessments require monitoring the emission and deposition of both the amount and types of Nr. This is especially important in heterogeneous landscapes, as different land-cover types emit particular forms of Nr to the atmosphere, which can impact ecosystems distinctively. Such assessments require high spatial resolution maps that also integrate temporal variations, and can only be feasibly achieved by using ecological indicators. Our aim was to rank land-cover types according to the amount and form of emitted atmospheric Nr in a complex landscape with multiple sources of N. To do so, we measured and mapped nitrogen concentration and isotopic composition in lichen thalli, which we then related to land-cover data. Results suggested that, at the landscape scale, intensive agriculture and urban areas were the most important sources of Nr to the atmosphere. Additionally, the ocean greatly influences Nr in land, by providing air with low Nr concentration and a unique isotopic composition. These results have important consequences for managing air pollution at the regional level, as they provide critical information for modeling Nr emission and deposition across regional as well as continental scales., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

During the last decades, awareness regarding persistent organic pollutants (POPs), such dioxins and furans (PCDD/Fs) and polycyclic aromatic hydrocarbons (PAHs), has become a cutting-edge topic, due to their toxicity, bioaccumulation and persistency in the environment. Monitoring of PCDD/Fs and PAHs in air and water has proven to be insufficient to capture deposition and effects of these compounds in the biota. To overcome this limitation, environmental biomonitoring using lichens and aquatic mosses, have aroused as promising tools. The main aim of this work is to provide a review of: i) factors that influence the interception and accumulation of POPs by lichens; ii) how lichens and aquatic bryophytes can be used to track different pollution sources and; iii) how can these biomonitors contribute to environmental health studies. This review will allow designing a set of guidelines to be followed when using biomonitors to assess environmental POP pollution., (Copyright © 2013 Elsevier Ltd. All rights reserved.)

One of the main drawbacks of using lichens to monitor atmospheric PAHs has been reported as the inexistence of studies aiming to translate PAH values in lichens into the atmospheric equivalents ones, in order to use this information for regulatory purposes. In this work, PAH concentrations in lichens were compared with PAH concentrations measured in a conventional active sampler in an outdoor environment for a 9-month span. Significant positive correlations between HMW-PAHs, Σ16 EPA-PAHs, and BaP equivalent concentrations in lichens and those in air (TSP) were found. Concentrations of Σ16 EPA-PAHs in lichens and air showed a seasonal variation, with highest values during winter and lowest values during summer. Meteorological variables - temperature, atmospheric pressure, relative humidity, and wind speed - showed to significantly influence PAH concentrations in both lichens and air. Based on the significant linear correlations, equations for translating PAH concentrations measured in lichens into equivalent ones for air were proposed for the first time, allowing a broader use of lichens' information regarding PAHs in monitoring schemes and decision-making., (Copyright © 2013 Elsevier Ltd. All rights reserved.)

Polycyclic aromatic hydrocarbons (PAH) are toxic compounds that have been classified by the International Agency for Research on Cancer as probable or possible human carcinogens. Human exposure to PAH is usually assessed by considering data from a single air monitoring station as being representative of a large region; however, air pollution levels change on small spatial scales and thus also affect environmental exposure. The use of environmental biomonitors is a useful tool to assess the levels of PAH with high spatial resolution. The aims of this study were to (1) assess human exposure to PAH in a petrochemical region in Portugal, integrating data from environmental biomonitors (lichens), air, and soil in a regional area, and (2) determine the health risks associated with exposure to PAH with high spatial resolution. Bearing this in mind, benzo[a]pyrene (BaP) equivalent concentrations in samples of soil, air, and lichens collected in the study region were used to assess human exposure through different pathways, including inhalation of air and soil particles, ingestion of soil, and dermal contact with soil. Human health risk was calculated through the Incremental Lifetime Cancer Risk (ILCR). BaP equivalent concentrations found in the region ranged from 6.9 to 46.05 ng BaPeq/g in lichens, from 16.45 to 162.02 ng BaPeq/g in soils, and from 0.02 to 0.16 ng BaPeq/m³ in air, indicative of high variability in this regional area. Human exposure to PAH varied between 976 and 42,877 ng BaPeq/d. When considering all exposure pathways, ILCR values were between 10⁻⁴ and 10⁻³. Considering only inhalation, ILCR values were between 10⁻⁶ and 10⁻⁵. The main risk seemed to arise from soil (either ingestion or inhalation of resuspended soil particles). The high spatial resolution of our environmental data allowed for detection of critical exposure levels at unexpected sites. Our results identified important areas where health studies on local populations need to be focused, and where environmental levels of PAH need to be monitored over time in order to protect human health.

Toxic polycyclic aromatic hydrocarbons (PAHs) can be found in wastewaters and sewages released from industries and/or urban areas. When discharged untreated to stream waters, they can be a problem to human health. This work represents the first attempt to use PAH and metal concentrations in aquatic moss transplants together with land-use information to identify water pollution sources in urban areas. To do this, the moss Fontinalis antipyretica was collected from a natural stream and transplanted to four different streams in a densely populated area of Lisbon, Portugal. After three months of exposure, mosses were collected and analyzed for metals and for the 16 priority PAHs recommended by the U.S. EPA. Urban streams seem to have a scattered contamination of 6-ring PAHs. Correlations among land-use, metal concentrations, and PAH concentrations indicated that areas occupied by activities of tertiary and industrial sectors had higher PAH concentrations in transplanted mosses, mainly for the sum of the 16 EPA-PAHs and for the 2-, 3- and 5-ringed PAHs, than areas occupied by urban and wooded areas. These PAHs were associated with enhanced Zn and Cu and land use activities that linked the sites to high traffic density. Industrial land use influences PAH concentration in water up to 1000 m of distance from the stream, whereas tertiary sector land use influences it up to 500 m.

The aim of this study was to validate lichens as biomonitors of PAH atmospheric deposition; for that, an inter-comparison between the PAH profile and concentrations intercepted in lichens with those of air, soil and pine needles was performed. The study was conducted in a petro-industrial area and the results showed that PAH profiles in lichens were similar to those of the air and pine needles, but completely different from those of soils. Lichens accumulated higher PAH concentrations when compared to the other environmental compartments and its concentrations were significantly and linearly correlated with concentrations of PAHs in soil; we showed that a translation of the lichen PAHs concentrations into regulatory standards is possible, fulfilling one of the most important requirements of using lichens as biomonitors. With lichens we were then able to characterize the air PAHs profile of urban, petro-industrial and background areas., (Copyright (c) 2009 Elsevier Ltd. All rights reserved.)

Sediment descriptors (grain size, total volatile solids, redox potential) and stable carbon and nitrogen isotopes were used to trace the origin of organic matter in a coastal area under multiple organic enrichment sources (urban outfall and a major estuary). The sediments fines content and total volatile solids were similar to outfall pre-operation period (1994), but the incorporation of terrestrial organic matter within the sediments located closer to the outfall was diagnosed by depleted (13)C values (-24.2+/-0.38 per thousand) and (15)N values (2.4+/-0.93 per thousand). Data also indicated depleted nitrogen signature at larger distances from the outfall than the carbon signature, due to confounding sediment grain size properties. Analysis in the bivalve Abra alba gave the same results for both isotopes and thus allowed a coherent interpretation of the spatial extent of the organic enrichment, highlighting the importance of bringing a biological element into the environmental assessment., (Copyright 2009 Elsevier Ltd. All rights reserved.)

The aim of this study was to compare the performance of two lichen species--Xanthoria parietina and Ramalina canariensis--as biomonitors of the toxic organic compounds dioxins and furans (PCDD/Fs). For that purpose, the concentrations and profiles of PCDD/Fs found in samples of these two lichen species were compared. Results showed that R. canariensis presented higher concentrations than X. parietina and that the PCDD/F homologue profiles were substantially different between these species. Xanthoria parietina appeared to be a more efficient interceptor of more chlorinated PCDD/Fs and also of particles, whereas R. canariensis mainly reflected less chlorinated PCDD/Fs. Results also showed that the PCDD/F profile of X. parietina differed from the one found in other foliose and fruticose lichen species. Despite the differences observed between the profiles of R. canariensis and X. parietina, the calibration of PCDD/F concentrations between the two species was achieved, allowing the biomonitoring of PCDD/Fs at a regional scale using both species simultaneously.

PAHs are toxic compounds emitted by several anthropogenic sources, which have a great impact on human health. We show, for the first time, how spatial models based on PAHs intercepted by lichens can be used for fingerprinting multisource atmospheric pollution in a regional area. Urban-industrial areas showed the highest atmospheric deposition of PAHs followed by urban > industrial > agricultural > forest Multivariate analysis of lichen data showed, for the first time, a clear distinction between various sources of PAHs in the same area: urban are dominated by 4-ring PAHs, forest by 3-ring PAHs, and industrial by 5- and 6-ring PAHs or by 2-ring PAHs (petrogenic or pyrogenic, respectively). Heavy metals were also used for supporting the fingerprinting of PAH sources, reinforcing the industrial origin of 5- and 6-ring PAHs and revealing their particular nature. The spatial structure of the models for different PAHs seems to be dependent on the following factors: size and hydrophilic character of different PAHs, type of emission sources (point or nonpoint), and dispersion associated with particulates of different sizes. Based on the long-term integration of PAHs in lichens, these spatial models will significantly improve our knowledge on the impact of PAH chronic-exposure to humans and ecosystems.

The objective of this work was to evaluate the spatial and temporal impact of dust-pollution in the vicinity of a cement industry, located in an area with dry climate. The spatial impact integrated over time was evaluated from the concentrations of Ca, Fe and Mg in in-situ Xanthoria parietina. The temporal pattern was assessed through one-month transplants of the lichen Ramalina canariensis. Four potential sources of atmospheric dust were evaluated: the limestone-quarry; the unpaved roads, the deposit area and the cement mill. Calcium concentration in lichens was considered the best cement-dust indicator. Different types of dust (clinker and grinded-limestone-dust) resulted in different time-patterns of Ca accumulation, which was also related with the different influence that wet and dry periods have in the lichen accumulation process. The dust pollution was found to be deposited locally and dependent on: the nature of dust particles and the volume and frequency of precipitation.