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In September 2020, the atmosphere was probed using the Optik Tu-134 aircraft laboratory over the waters of the Arctic Ocean seas: the Barents, Kara, Laptev, East Siberian, Chukchi, and Bering seas. Unique samples of atmospheric aerosols were collected at the altitudes from 200 to 10,000 m including samples in impingers for identification and genetic analysis of culturable microorganisms. The paper presents data on the concentrations and diversity of bacteria and fungi isolated by seeding 24 samples of atmospheric aerosols collected at different altitudes over the Arctic seas of Russia. The main morphophysiological, biochemical and genomic characteristics were obtained for 152 bacterial cultures, and the taxonomic groups they belong to were determined.

To contribute to the comprehensive study of atmospheric pollution in Siberia, aircraft soundingwas carried out in Northwestern Siberian along the following route: Novosibirsk - Surgut - Igarka - Novosibirsk. This work was aimed at studying the quantity and representation of culturable microorganisms and other biogenic components of the atmosphere at altitudes up to 8,000 m. The air samples were collected to impingers (flow rate 50 ± 5 L/min) where 50 ml of Hanks’ solution (ICN Biomedicals) was used as the sorbing liquid and applied on the fibrous filters. The concentration of biogenic material was recorded, and the concentration and diversity of culturable microorganisms were determined in total protein samples. It was found that the samples of atmospheric air contained 158 mesophilic and psychrophilic microorganisms represented by such genera as Micrococcus, Staphylococcus, Bacillus, Nocardia, Arthrobacter, and Rhodococcus. Also, Actinomycete, yeast and fungal cultures were also isolated. Non-sporiferous bacteria were widely presented at all altitudes. Enzymatic activity of the isolated microorganisms and a number of pathogenicity factors present were investigated. The data on quantity and representation of culturable microorganisms and other biogenic components at altitudes up to 8000 m in Northwestern Siberia were obtained for the first time. It is a significant contribution to the study of the atmosphere of this region. The patterns of the observed biodiversity of microorganisms, associated with the sampling altitude or geographic location, should be determined infurther research.

In 2020, a unique experiment, which had ever been implemented either in the former USSR or in modern-day Russia, was carried out in the Russian Arctic by means of the Optik Tu-134 aircraft laboratory operated by IAO SB RAS. The airborne measurement campaign was conducted on September 4-17 over all seas and coastal regions of the Russian sector of the Arctic, including northern part of the Bering Sea.During the flights, in situ measurements of CO, CO2, CH4, NO, NO2, SO2, O3, aerosols, and black carbon (BC) were performed. Air samples were taken to determine organic and inorganic compounds and biological material in aerosol particles. A remote sensing of the water turbidity in the upper sea layers was conducted by means of the LOZA-2 lidar that allowed a concentration of plankton to be derived there. Spectral characteristics of the water and underlying coastal surfaces were measured using a spectroradiometer.The primary analysis of the obtained data showed that concentrations of CO, NO, NO2, SO2, O3, aerosols, and BC during the experiment were low that is typical for background regions. CO2 mixing ratios in the lowest part of the troposphere above seas were lower than aloft. As compared with coastal areas, concentration of methane over all the seas of the Arctic sector and the Bering Sea was higher.We would like to acknowledge our colleagues from the following organizations for their assistance in organizing and conducting this campaign, and in particular, Laboratoire des sciences du climat et de l'environnement and Laboratoire atmosphères, milieux, observations spatiales (France); Finnish Meteorological Institute and Institute for Atmospheric and Earth System Research, University of Helsinki (Finland); Center for Global Environmental Research at the National Institute for Environmental Studies (Japan); the National Oceanic and Atmospheric Administration, US Department of Commerce (USA); Max-Planck-Institute for Biochemistry (Germany); and University of Reading (UK).

One of the most important components of atmospheric aerosols are microorganisms. Therefore, it is necessary to assess the hazard to humans, both from individual microorganisms which are present in atmospheric bioaerosols as well as from their pool. An approach for determining the hazard of bacteria and yeasts found in atmospheric bioaerosols for humans has previously been proposed. The purpose of this paper is to compare our results for 2006&ndash, 2008 with the results of studies obtained in 2012&ndash, 2016 to identify changes in the characteristics of bioaerosols occurring over a decade in the south of Western Siberia. Experimental data on the growth, morphological and biochemical properties of bacteria and yeasts were determined for each isolate found in bioaerosol samples. The integral indices of the hazards of bacteria and yeast for humans were constructed for each isolate based on experimentally determined isolate characteristics according to the approach developed by authors in 2008. Data analysis of two datasets showed that hazard to humans of culturable microorganisms in the atmospheric aerosol in the south of Western Siberia has not changed significantly for 10 years (trends are undistinguishable from zero with a confidence level of more than 95%) despite a noticeable decrease in the average annual number of culturable microorganisms per cubic meter (6&ndash, 10 times for 10 years).

The atmospheric air quality is determined by the concentrations of some gaseous pollutants and mass concentrations of aerosol particles of different sizes. A wide range of atmospheric pollutants in both gaseous and aerosol phases was studied in the vicinity of Gelendzhik in July 2009, simultaneously at several land sites, in the water area of the bay, and at altitudes of up to 2200m. No such complex experiments were carried out in that region before. The following characteristics of the atmospheric aerosol (3 nm–32 μm in size) were studied: elemental composition of particles (23 chemical elements) and concentrations of polyaromatic hydrocarbons (14 compounds), unsaturated hydrocarbons, total protein, biotoxins, and culturable microorganisms. The concentration fields of different air pollutants and the complex air pollution index were constructed using mathematical models of pollutant propagation and data on the hydrometeorological conditions during the period of measurements. The sources of aerosols in the region were detected from the study of the chemical composition of airborne particles. The results allowed us to estimate air pollutants and to calculate the complex air pollution index for the Gelendzhik area. The daily average concentrations of all the pollutants were compared to the daily average maximum permissible concentrations. All these concentrations were less than daily average maximum permissible concentrations. The complex air pollution index did not exceed 1. Hence, the air in the vicinity of Gelendzhik did not contain any significant pollutants in the period under study.

The paper presents unique data of a 12-year (2001-2012) monitoring of the mass concentration of atmospheric aerosol and concentrations of organic carbon (OC), elemental carbon (EC), and total protein (TP) in it at the Klyuchi site (Novosibirsk region). It was found that aerosol mass concentrations, OC, and OC/EC ratio have an increasing trend, and TP has a decreasing trend. Seasonal changes in these concentrations and OC/EC and TP/OC ratios are also identified. The comparison of data obtained and recently published for other Asian and European regions show a good agreement between the values and trends for certain regions and the absence of agreement for other regions.

This paper describes a new method for precise determination of viral aerosol lethal dose (ALD 50 ) and reports the results obtained for birds exposed by H5N1 Avian influenza A strain under controlled laboratory conditions. The reported method utilizes our recently developed personal bioaerosol sampler capable of monitoring viable virus concentration in the ambient air. Up to six laboratory animals could be located in a specially designed aerosol chamber and exposed in parallel by an airborne strain of interest to generate the amount of data sufficient for representative statistical analysis. A concentration of viable airborne virus was measured by the personal aerosol samplers directly in the breathing zone of each particular bird. The results show a very low inter-sampler variation used for each particular run, which was confirmed by a single factor ANOVA test undertaken for all six personal samplers involved in each experiment. As was shown, the difference in amount of viruses collected by all samplers during each particular run was not statistically significant. It was found that the ALD 50 for approximately 400-g birds exposed to H5N1 Avian influenza A strain A/Chicken/Suzdalka/Nov-11/2005 was around 26.5 FFU (focus forming units).

A new personal bioaerosol sampler, which was found suitable for continuous long-term (up to 8 h) monitoring of airborne bacteria, fungi, and viruses, was recently developed. A range of investigations under controlled laboratory conditions verified the performance of the device for both stress sensitive and robust viral particles. To decrease the detection time, the next step of the technique development was related to utilization of the sampler in combination with polymerase chain reaction (PCR) technology in the laboratory. The combined device was found to be fully feasible with the corresponding decrease of the detection time from a few days to 2.5 h. In addition, the results for targeted microorganisms were not affected by background biological particles and cross-reaction was not observed. The current study is the first trial to use the new combined device, i. e., sampler-conventional PCR device, for monitoring airborne viruses in the field. The monitoring procedures were performed in hospital infection wards with patients suffering from mumps and measles diseases to detect the corresponding disease causing viruses in the ambient air. The results for the existence of the airborne viruses were obtained for both strains. A simple procedure is also suggested for enumeration of microbial contamination in the ambient air.

A novel bioaerosol sampling technique, which utilizes the bubbling process in the collection fluid, has recently been developed and found feasible for a long-term personal sampling of airborne bacteria and fungal spores as it maintained high physical collection efficiency and high microbial recovery rate for robust and stress-sensitive microorganisms. Further tests have shown that the new technique also has potential to collect viable airborne viruses, particularly when utilized for a short-term sampling of robust strains. As the short-term sampling has a limited application for assessing personal exposure in bioaerosol-contaminated environments, the present study was undertaken to investigate the feasibility of the “bubbler” for a long-term monitoring of viable airborne viruses. Liquid droplets containing Vaccinia virions (that simulate Variola, a causative agent of smallpox) were aerosolized with a Collison nebulizer into a 400-liter test chamber, from which the droplets were collected by three identica...

The State Research Center of Virology and Biotechnology “Vector” and the Institute of Optics SB RAS are performing systematic study of tropospheric bioaerosols in the South of Western Siberia. The work summarizes the results of analysis of some physical characteristics of the data array on bioaerosol concentration obtained with an airplane laboratory at the altitudes of 0.5, 1, 1.5, 2, 3, 4, 5.5 and 7 km. The flights were performed during the last 10 days of each month in 1999–2003. It was shown that the concentration of total protein aerosols obeyed the laws of continual statistics, and the concentration of culturable microorganism aerosols obeyed the laws of discrete statistics. The analysis of correlation coefficients and cross correlation of bioaerosol concentration fields was performed. Wavelet analysis of the data showed that variations of tropospheric bioaerosol concentration were mainly determined by typical seasonal processes with periods of 12, 6, 4 and 8–9 months. Seasonal variations cause approximately 80% of the total dispersion of variations of total protein concentration, and the amplitudes of variations of culturable microorganism concentration are small as compared with the constant component. Harmonic analysis of bioaerosol concentration fields was performed. Harmonics that are significant in wavelet spectra for all time series were taken for the approximation of the series. It was revealed that four-month periodicity was internal and was caused by a wave spreading from above with a vertical rate of about 4 km/month.

While various sampling methods exist for collecting and enumerating airborne bacteria and fungi, no credible methodology has yet been developed for airborne viruses. A new sampling method for monitoring the personal exposure to bioaerosol particles has recently been developed and evaluated with bacteria and fungi. In this method, bacterial/fungal aerosol is aspirated and transported through a porous medium, which is submerged into a liquid layer. As the air is split into numerous bubbles, the particles are scavenged by these bubbles and effectively removed. The current feasibility study was initiated to evaluate the efficiency of the new personal sampler prototype ("bubbler") with airborne viable viruses. Two common viral strains, Influenza (stress-sensitive) and Vaccinia (robust), were aerosolized in the test chamber and collected by two identical "bubblers" that operated simultaneously for a duration of upto 5 min. A virus maintenance liquid, proven to be the optimum collecting environment for the test organisms, was used as a collection fluid. After sampling, the collecting fluid was analyzed and the viral recovery rate was determined. The overall recovery (affected not only by the sampling but also by the aerosolization and the aerosol transport) was 20% for Influenza virus and 89% for Vaccinia virus. The new sampling method was found feasible for the collection and enumeration of robust airborne viruses.

Due to recent SARS related issues (Science 300 (5624) 1394; Nature 423 (2003) 240; Science 300 (5627) 1966), the development of reliable airborne virus monitoring procedures has become galvanized by an exceptional sense of urgency and is presently in a high demand (In: Cox, C.S., Wathers, C.M. (Eds.), Bioaerosols Handbook, Lewis Publishers, Boca Raton, FL, 1995, pp. 247-267). Based on engineering control method (Aerosol Science and Technology 31 (1999) 249; 35 (2001) 852), which was previously applied to the removal of particles from gas carriers, a new personal bioaerosol sampler has been developed. Contaminated air is bubbled through porous medium submerged into liquid and subsequently split into multitude of very small bubbles. The particulates are scavenged by these bubbles, and, thus, effectively removed. The current study explores its feasibility for monitoring of viable airborne SARS virus. It was found that the natural decay of such virus in the collection fluid was around 0.75 and 1.76 lg during 2 and 4 h of continuous operation, respectively. Theoretical microbial recovery rates of higher than 55 and 19% were calculated for 1 and 2 h of operation, respectively. Thus, the new sampling method of direct non-violent collection of viable airborne SARS virus into the appropriate liquid environment was found suitable for monitoring of such stress sensitive virus.