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4 results on '"Diacétyle"'

1. Development and Use of a Screening Procedure for Production of (alpha)-Acetolactate by Lactococcus lactis subsp. lactis biovar diacetylactis Strains

Author

Christophe Monnet, P. Schmitt, Charles Diviès, Génie et Microbiologie des Procédés Alimentaires (GMPA), and Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G)

Subjects
calorimétrie, bactérie lactique, Biovar, Mutagenesis (molecular biology technique), Applied Microbiology and Biotechnology, Microbiology, Agar plate, 03 medical and health sciences, chemistry.chemical_compound, décarboxylase, LACTOCOCCUS LACTIS, mutant, citrate, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, chemistry.chemical_classification, BIOTECHNOLOGIE, 0303 health sciences, alpha acétolactate, Ecology, biology, 030306 microbiology, Lactococcus lactis, food and beverages, biology.organism_classification, Streptococcaceae, lait, Diacetyl, 3. Good health, Enzyme, chemistry, Biochemistry, génie génétique, diacétyle, bacteria, Bacteria, Research Article, Food Science, Biotechnology
Abstract

A method was developed to screen and isolate mutagenized Lactococcus lactis subsp. lactis biovar diacetylactis strains accumulating (alpha)-acetolactate. This compound is accumulated by (alpha)-acetolactate decarboxylase-deficient strains and undergoes spontaneous degradation into diacetyl on agar plates. The diacetyl produced is detected by a colorimetric reaction yielding a red halo around the colonies.

Published
1997
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2. Diacetyl and alpha-acetolactate overproduction by Lactococcus lactis subsp. lactis biovar diacetylactis mutants that are deficient in alpha-acetolactate decarboxylase and have a low lactate dehydrogenase activity

Author

Monnet, Christophe, Aymes, F., Corrieu, G., Génie et Microbiologie des Procédés Alimentaires (GMPA), and Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G)

Subjects
[SDV.EE]Life Sciences [q-bio]/Ecology, environment, enzyme, acétolactate, bactérie lactique, activité enzymatique, diacétyle, LACTOCOCCUS LACTIS, oxygène, mutation, lait, composé aromatique, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2000

3. Effect of alpha-acetolactate inactivation on alpha-acetolactate and diacetyl production by Lactococcus lactis subsp. lactis biovar diacetylactis

Author

Aymes, F., Monnet, Christophe, Corrieu, Georges, Génie et Microbiologie des Procédés Alimentaires (GMPA), and Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G)

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, pH, bactérie lactique, Biotechnologies, lait, composé aromatique, acétoïne, enzyme, acétolactate, diacétyle, LACTOCOCCUS LACTIS, oxygène, mutation, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1999

4. Isolation and properties of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 mutants producing diacetyl and acetoin from glucose

Author

Christophe Monnet, Georges Corrieu, Hassina Boumerdassi, Michel Desmazeaud, Génie et Microbiologie des Procédés Alimentaires (GMPA), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G), Bactéries Lactiques et Pathogènes Opportunistes (UBLO), and Institut National de la Recherche Agronomique (INRA)

Subjects
bactérie lactique, pyruvate, éthanol, Diacetyl, anaérobiose, Applied Microbiology and Biotechnology, Microbiology, acétoïne, 03 medical and health sciences, chemistry.chemical_compound, Lactate dehydrogenase, LACTOCOCCUS LACTIS, mutant, industrie laitière, glucose, DNA Primers, 030304 developmental biology, chemistry.chemical_classification, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, 0303 health sciences, BIOTECHNOLOGIE, Base Sequence, L-Lactate Dehydrogenase, Ecology, Strain (chemistry), biology, 030306 microbiology, Acetoin, Lactococcus lactis, Metabolism, NAD, biology.organism_classification, Streptococcaceae, lactate déshydrogénase, Kinetics, Enzyme, chemistry, Biochemistry, génie génétique, Mutation, aérobiose, diacétyle, Research Article, Food Science, Biotechnology
Abstract

Following treatment with the mutagen N-methyl-N'-nitro-N-nitrosoguanidine, three mutants of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 that produced diacetyl and acetoin from glucose were isolated. The lactate dehydrogenase activity of these mutants was strongly attenuated, and the mutants produced less lactate than the parental strain. The kinetic properties of lactate dehydrogenase of strain CNRZ 483 and the mutants revealed differences in the affinity of the enzyme for pyruvate, NADH, and fructose-1,6-diphosphate. When cultured aerobically, strain CNRZ 483 transformed 2.3% of glucose to acetoin and produced no diacetyl or 2,3-butanediol. Under the same conditions, mutants 483L1, 483L2, and 483L3 transformed 42.0, 78.9, and 75.8%, respectively, of glucose to C4 compounds (diacetyl, acetoin, and 2,3-butanediol). Anaerobically, strain CNRZ 483 produced no C4 compounds, while mutants 483L1, 483L2, and 483L3 transformed 2.0, 37.0, and 25.8% of glucose to acetoin and 2,3-butanediol. In contrast to the parental strain, the NADH balance showed that the mutants regenerated most of the NAD via NADH oxidase under aerobic conditions and by ethanol production under anaerobic conditions.

Published
1997

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