1. Mechanism‐Guided Computational Design of ω‐Transaminase by Reprograming of High‐Energy‐Barrier Steps.
- Author
-
Yang, Lin, Zhang, Kaiyue, Xu, Meng, Xie, Youyu, Meng, Xiangqi, Wang, Hualei, and Wei, Dongzhi
- Subjects
- *
TURNOVER frequency (Catalysis) , *GOVERNMENT aid , *ENZYME kinetics , *QUANTUM mechanics , *CATALYTIC activity , *TRANSITION state theory (Chemistry) , *PROTEIN engineering - Abstract
ω‐Transaminases (ω‐TAs) show considerable potential for the synthesis of chiral amines. However, their low catalytic efficiency towards bulky substrates limits their application, and complicated catalytic mechanisms prevent precise enzyme design. Herein, we address this challenge using a mechanism‐guided computational enzyme design strategy by reprograming the transition and ground states in key reaction steps. The common features among the three high‐energy‐barrier steps responsible for the low catalytic efficiency were revealed using quantum mechanics (QM). Five key residues were simultaneously tailored to stabilize the rate‐limiting transition state with the aid of the Rosetta design. The 14 top‐ranked variants showed 16.9–143‐fold improved catalytic activity. The catalytic efficiency of the best variant, M9 (Q25F/M60W/W64F/I266A), was significantly increased, with a 1660‐fold increase in kcat/Km and a 1.5–26.8‐fold increase in turnover number (TON) towards various indanone derivatives. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF