1. Sample-to-Answer Robotic ELISA
- Author
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Shiva Emami, Zecong Fang, Tingrui Pan, Chuqing Zhou, Cunyi Zhao, Ameer Y. Taha, Xiyan Mai, and Gang Sun
- Subjects
Immunoassay ,Chemistry ,business.industry ,Machine vision ,Nanofibrous membrane ,Interface (computing) ,Sample (material) ,Microfluidics ,Enzyme-Linked Immunosorbent Assay ,Microfluidic Analytical Techniques ,Modular design ,Article ,Analytical Chemistry ,Highly sensitive ,Sample volume ,Robotic Surgical Procedures ,Microfluidic chip ,Embedded system ,Humans ,Colorimetry ,business - Abstract
Enzyme-linked immunosorbent assays (ELISA), as one of the most used immunoassays, have been conducted ubiquitously in hospitals, research laboratories, etc. However, the conventional ELISA procedure is usually laborious, occupies bulky instruments, consumes lengthy operation time, and relies considerably on the skills of technicians, and such limitations call for innovations to develop a fully automated ELISA platform. In this paper, we have presented a system incorporating a robotic-microfluidic interface (RoMI) and a modular hybrid microfluidic chip that embeds a highly sensitive nanofibrous membrane, referred to as Robotic ELISA, to achieve human-free sample-to-answer ELISA tests in a fully programmable and automated manner. It carries out multiple bioanalytical procedures to replace the manual steps involved in classic ELISA operations, including the pneumatically driven high-precision pipetting, efficient mixing and enrichment enabled by back-and-forth flows, washing, as well as integrated machine vision for colorimetric readout. The Robotic ELISA platform has achieved a low limit of detection (LOD) of 0.1 ng/mL in the detection of a low sample volume (15 μL) of chloramphenicol (CAP) within 20 min without human intervention, which is significantly faster than that of the conventional ELISA procedure. Benefiting from its modular design and automated operations, the Robotic ELISA platform has great potential to be deployed for a broad range of detections in various resource-limited settings or high-risk environments, where human involvement needs to be minimized, while the testing timeliness, consistency and sensitivity are all desired.
- Published
- 2021