17 results on '"Sandgren, Mats"'
Search Results
2. Quantum mechanical calculations suggest that lytic polysaccharide monooxygenases use a copper-oxyl, oxygen-rebound mechanism
- Author
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Kim, Seonah, Stahlber, Jerry, Sandgren, Mats, Paton, Robert S., and Beckham, Gregg T.
- Published
- 2014
3. Glycosylated linkers in multimodular lignocellulosedegrading enzymes dynamically bind to cellulose
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Payne, Christina M., Resch, Michael G., Chen, Liqun, Crowley, Michael F., Himmel, Michael E., Taylor, Larry E., Sandgren, Mats, Ståhlberg, Jerry, Stals, Ingeborg, Tan, Zhongping, and Beckham, Gregg T.
- Published
- 2013
4. Inhibition of Cytosine Methylation Allows Efficient Cloning of T-DNA Tagged Plant DNA, of Arabidopsis thaliana by Plasmid Rescue
- Author
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Mandal, Abul, Sandgren, Mats, and Palva, E. Tapio
- Published
- 1994
5. Enantioselective Binding of Propranolol and Analogues Thereof to Cellobiohydrolase Cel7A.
- Author
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Hamark, Christoffer, Pendrill, Robert, Landström, Jens, Dotson Fagerström, Alexandra, Sandgren, Mats, Ståhlberg, Jerry, and Widmalm, Göran
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CELLULOSE 1,4-beta-cellobiosidase ,CELLOBIOSE ,PROPRANOLOL ,CATALYSTS ,ENANTIOMERS - Abstract
At the catalytic site for the hydrolysis of cellulose the enzyme cellobiohydrolase Cel7A binds the enantiomers of the adrenergic beta‐blocker propranolol with different selectivity. Methyl‐to‐hydroxymethyl group modifications of propranolol, which result in higher affinity and improved selectivity, were herein studied by 1H,1H and 1H,13C scalar spin–spin coupling constants as well as utilizing the nuclear Overhauser effect (NOE) in conjunction with molecular dynamics simulations of the ligands per se, which showed the presence of all‐antiperiplanar conformations, except for the one containing a vicinal oxygen–oxygen arrangement governed by the gauche effect. For the ligand–protein complexes investigated by NMR spectroscopy using, inter alia, transferred NOESY and saturation‐transfer difference (STD) NMR experiments the S‐isomers were shown to bind with a higher affinity and a conformation similar to that preferred in solution, in contrast to the R‐isomer. The fact that the S‐form of the propranolol enantiomer is pre‐arranged for binding to the protein is also observed for a crystal structure of dihydroxy‐(S)‐propranolol and Cel7A presented herein. Whereas the binding of propranolol is entropy driven, the complexation with the dihydroxy analogue is anticipated to be favored also by an enthalpic term, such as for its enantiomer, that is, dihydroxy‐(R)‐propranolol, because hydrogen‐bond donation replaces the corresponding bonding from hydroxyl groups in glucosyl residues of the natural substrate. In addition to a favorable entropy component, albeit lesser in magnitude, this represents an effect of enthalpy‐to‐entropy compensation in ligand–protein interactions. Ligand–protein interactions: Conformational preferences of propranolol and dihydroxypropranolol in aqueous solvent were elucidated by NMR spectroscopy and MD simulations (see figure). [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
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6. The kinetics of TEM1 antibiotic degrading enzymes that are displayed on Ure2 protein nanofibrils in a flow reactor.
- Author
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Schmuck, Benjamin, Sandgren, Mats, and Härd, Torleif
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ENZYME kinetics , *NANOSTRUCTURED materials , *TURNOVER frequency (Catalysis) , *SACCHAROMYCES cerevisiae , *PROTEIN structure - Abstract
Enzymatic functionalization of cross-β structured protein nanofibrils has hitherto resulted in a severe reduction of the catalytic efficiency of high turnover biocatalysts. It has been speculated that steric restrictions and mass transport pose limits on the attached enzymes, but detailed kinetics analyzing this have not yet been reported. For a more comprehensive understanding, we studied protein nanofibrils endowed with TEM1, a β-lactamase from Escherichia coli. The packing density of TEM1 along the fibrils was controlled by co-fibrillation; in other words, the N-terminal ureidosuccinate transporter Ure2(1–80) from Saccharomyces cerevisiae was simultaneously aggregated with the chimeric proteins TEM1-Ure2(1–80). The mature fibrils were trapped in a column, and the rate of ampicillin hydrolysis was recorded using a continuous substrate flow. The turnover rate was plotted as a function of substrate molecules available per enzyme per second, which demonstrated that an elevated substrate availability counteracts mass transport limitations. To analyze this data set, we derived a kinetic model, which makes it possible to easily characterize and compare enzymes packed in columns. The functional TEM1 nanofibrils possess 80% of the catalytic turnover rate compared to free TEM1 in solution. Altogether, we have created protein nanofibrils that can effectively hydrolyze β-lactam antibiotic contaminations and provided a groundwork strategy for other highly functional enzymatic nanofibrils. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
7. Rational Design of Spider Silk Materials Genetically Fused with an Enzyme.
- Author
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Jansson, Ronnie, Courtin, Christophe M., Sandgren, Mats, and Hedhammar, My
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ENZYMES ,SPIDER silk ,ENCAPSULATION (Catalysis) ,XYLANASES ,GENETIC engineering ,MOLECULAR self-assembly - Abstract
Enzyme immobilization is an attractive route for achieving catalytically functional surfaces suitable for both continuous and repeated use. Herein, genetic engineering is used to combine the catalytic ability of a xylanase with the self-assembly properties of recombinant spider silk, realizing silk materials with enzymatic activity. Under near-physiological conditions, soluble xylanase-silk fusion proteins assembled into fibers displaying catalytic activity. Also, a xylanase-silk protein variant with the silk part miniaturized to contain only the C-terminal domain of the silk protein formed fibers with catalytic activity. The repertoire of xylanase-silk formats is further extended to include 2D surface coatings and 3D foams, also being catalytically active, showing the versatile range of possible silk materials. The stability of the xylanase-silk materials is explored, demonstrating the possibility of storage, reuse, and cleaning with ethanol. Interestingly, fibers can also be stored dried with substantial residual activity after rehydration. Moreover, a continuous enzymatic reaction using xylanase-silk is demonstrated, making enzymatic batch reactions not the sole possible implementation. The proof-of-concept for recombinantly produced enzyme-silk, herein shown with a xylanase, implies that also other enzymes can be used in similar setups. It is envisioned that the concept of enzyme-silk can find its applicability in, for example, multienzyme reaction systems or biosensors. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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8. X-ray crystal structures of Phanerochaete chrysosporium Laminarinase 16A in complex with products from lichenin and laminarin hydrolysis.
- Author
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Vasur, Jonas, Kawai, Rie, Andersson, Evalena, Igarashi, Kiyohiko, Sandgren, Mats, Samejima, Masahiro, and Ståhlberg, Jerry
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PHANEROCHAETE ,GLYCOSIDES ,HYDROLASES ,HYDROLYSIS ,X-ray crystallography ,ENZYMES - Abstract
The 1,3(4)- β-d-glucanases of glycoside hydrolase family 16 provide useful examples of versatile yet specific protein–carbohydrate interactions. In the present study, we report the X-ray structures of the 1,3(4)- β-d-glucanase Phanerochaete chrysosporium Laminarinase 16A in complex with β-glucan products from laminarin (1.6 Å) and lichenin (1.1 Å) hydrolysis. The G6G3G3G glucan, in complex with the enzyme, showed a β-1,6 branch in the acceptor site. The G4G3G ligand–protein complex showed that there was no room for a β-1,6 branch in the −1 or −2 subsites; furthermore, the distorted residue in the −1 subsite and the glucose in the −2 subsite required a β-1,3 bond between them. These are the first X-ray crystal structures of any 1,3(4)- β-d-glucanase in complex with glucan products. They provide details of both substrate and product binding in support of earlier enzymatic evidence. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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9. Structural and biochemical studies of GH family 12 cellulases: improved thermal stability, and ligand complexes
- Author
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Sandgren, Mats, Ståhlberg, Jerry, and Mitchinson, Colin
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ENZYMES , *PROTEINS , *ENZYMOLOGY , *CATALYSTS - Abstract
Abstract: In this review we will describe how we have gathered structural and biochemical information from several homologous cellulases from one class of glycoside hydrolases (GH family 12), and used this information within the framework of a protein-engineering program for the design of new variants of these enzymes. These variants have been characterized to identify some of the positions and the types of mutations in the enzymes that are responsible for some of the biochemical differences in thermal stability and activity between the homologous enzymes. In this process we have solved the three-dimensional structure of four of these homologous GH 12 cellulases: Three fungal enzymes, Humicola grisea Cel12A, Hypocrea jecorina Cel12A and Hypocrea schweinitzii Cel12A, and one bacterial, Streptomyces sp. 11AG8 Cel12A. We have also determined the three-dimensional structures of the two most stable H. jecorina Cel12A variants. In addition, four ligand-complex structures of the wild-type H. grisea Cel12A enzyme have been solved and have made it possible to characterize some of the interactions between substrate and enzyme. The structural and biochemical studies of these related GH 12 enzymes, and their variants, have provided insight on how specific residues contribute to protein thermal stability and enzyme activity. This knowledge can serve as a structural toolbox for the design of Cel12A enzymes with specific properties and features suited to existing or new applications. [Copyright &y& Elsevier]
- Published
- 2005
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10. Crystal Complex Structures Reveal How Substrate is Bound in the -4 to the +2 Binding Sites of Humicola grisea Cel12A
- Author
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Sandgren, Mats, Berglund, Gunnar I., Shaw, Andrew, Ståhlberg, Jerry, Kenne, Lennart, Desmet, Tom, and Mitchinson, Colin
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BINDING sites , *ENZYMES , *CELLULASE , *CRYSTALLOIDS (Botany) - Abstract
As part of an ongoing enzyme discovery program to investigate the properties and catalytic mechanism of glycoside hydrolase family 12 (GH 12) endoglucanases, a GH family that contains several cellulases that are of interest in industrial applications, we have solved four new crystal structures of wild-type Humicola grisea Cel12A in complexes formed by soaking with cellobiose, cellotetraose, cellopentaose, and a thio-linked cellotetraose derivative (G2SG2). These complex structures allow mapping of the non-covalent interactions between the enzyme and the glucosyl chain bound in subsites -4 to +2 of the enzyme, and shed light on the mechanism and function of GH 12 cellulases. The unhydrolysed cellopentaose and the G2SG2 cello-oligomers span the active site of the catalytically active H.grisea Cel12A enzyme, with the pyranoside bound in subsite -1 displaying a skew boat conformation. After soaking in cellotetraose, the cello-oligomer that is found bound in site -4 to -1 contains a β-1,3-linkage between the two cellobiose units in the oligomer, which is believed to have been formed by a transglycosylation reaction that has occurred during the ligand soak of the protein crystals. The close fit of this ligand and the binding sites occupied suggest a novel mixed β-glucanase activity for this enzyme. [Copyright &y& Elsevier]
- Published
- 2004
- Full Text
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11. Machine learning reveals sequence-function relationships in family 7 glycoside hydrolases.
- Author
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Gado, Japheth E., Harrison, Brent E., Sandgren, Mats, Ståhlberg, Jerry, Beckham, Gregg T., and Payne, Christina M.
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MACHINE learning , *FAMILY relations , *CATALYTIC domains , *RANDOM forest algorithms , *MOIETIES (Chemistry) , *GLYCOSIDASES , *ENZYMES - Abstract
Family 7 glycoside hydrolases (GH7) are among the principal enzymes for cellulose degradation in nature and industrially. These enzymes are often bimodular, including a catalytic domain and carbohydrate-binding module (CBM) attached via a flexible linker, and exhibit an active site that binds cello-oligomers of up to ten glucosyl moieties. GH7 cellulases consist of two major subtypes: cellobiohydrolases (CBH) and endoglucanases (EG). Despite the critical importance of GH7 enzymes, there remain gaps in our understanding of how GH7 sequence and structure relate to function. Here, we employed machine learning to gain data-driven insights into relationships between sequence, structure, and function across the GH7 family. Machine-learning models, trained only on the number of residues in the active-site loops as features, were able to discriminate GH7 CBHs and EGs with up to 99% accuracy, demonstrating that the lengths of loops A4, B2, B3, and B4 strongly correlate with functional subtype across the GH7 family. Classification rules were derived such that specific residues at 42 different sequence positions each predicted the functional subtype with accuracies surpassing 87%. A random forest model trained on residues at 19 positions in the catalytic domain predicted the presence of a CBM with 89.5% accuracy. Our machine learning results recapitulate, as top-performing features, a substantial number of the sequence positions determined by previous experimental studies to play vital roles in GH7 activity. We surmise that the yet-to-be-explored sequence positions among the top-performing features also contribute to GH7 functional variation and may be exploited to understand and manipulate function. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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12. Evolution and functional characterization of pectate lyase PEL12, a member of a highly expanded Clonostachys rosea polysaccharide lyase 1 family.
- Author
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Atanasova, Lea, Dubey, Mukesh, Grujić, Marica, Gudmundsson, Mikael, Lorenz, Cindy, Sandgren, Mats, Kubicek, Christian P., Jensen, Dan Funck, and Karlsson, Magnus
- Subjects
PECTATE lyase ,DNA copy number variations ,PHYTOPATHOGENIC fungi ,PECTINS ,ENZYMES - Abstract
Background: Pectin is one of the major and most complex plant cell wall components that needs to be overcome by microorganisms as part of their strategies for plant invasion or nutrition. Microbial pectinolytic enzymes therefore play a significant role for plant-associated microorganisms and for the decomposition and recycling of plant organic matter. Recently, comparative studies revealed significant gene copy number expansion of the polysaccharide lyase 1 (PL1) pectin/pectate lyase gene family in the Clonostachys rosea genome, while only low numbers were found in Trichoderma species. Both of these fungal genera are widely known for their ability to parasitize and kill other fungi (mycoparasitism) and certain species are thus used for biocontrol of plant pathogenic fungi. Results: In order to understand the role of the high number of pectin degrading enzymes in Clonostachys, we studied diversity and evolution of the PL1 gene family in C. rosea compared with other Sordariomycetes with varying nutritional life styles. Out of 17 members of C. rosea PL1, we could only detect two to be secreted at acidic pH. One of them, the pectate lyase pel12 gene was found to be strongly induced by pectin and, to a lower degree, by polygalacturonic acid. Heterologous expression of the PEL12 in a PL1-free background of T. reesei revealed direct enzymatic involvement of this protein in utilization of pectin at pH 5 without a requirement for Ca
2+ . The mutants showed increased utilization of pectin compounds, but did not increase biocontrol ability in detached leaf assay against the plant pathogen Botrytis cinerea compared to the wild type. Conclusions: In this study, we aimed to gain insight into diversity and evolution of the PL1 gene family in C. rosea and other Sordariomycete species in relation to their nutritional modes. We show that C. rosea PL1 expansion does not correlate with its mycoparasitic nutritional mode and resembles those of strong plant pathogenic fungi. We further investigated regulation, specificity and function of the C. rosea PEL12 and show that this enzyme is directly involved in degradation of pectin and pectin-related compounds, but not in C. rosea biocontrol. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
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13. Coupled chemistry kinetics demonstrate the utility of functionalized Sup35 amyloid nanofibrils in biocatalytic cascades.
- Author
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Schmuck, Benjamin, Gudmundsson, Mikael, Härd, Torleif, and Sandgren, Mats
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XYLANASES , *CHEMISTRY , *SURFACE area , *ENZYMES , *PROOF of concept , *SECRETASE inhibitors - Abstract
Concerns over the environment are a central driver for designing cell-free enzymatic cascade reactions that synthesize non-petrol-based commodity compounds. An often-suggested strategy that would demonstrate the economic competitiveness of this technology is recycling of valuable enzymes through their immobilization. For this purpose, amyloid nanofibrils are an ideal scaffold to realize chemistry-free covalent enzyme immobilization on a material that offers a large surface area. However, in most instances, only single enzyme-functionalized amyloid fibrils have so far been studied. To embark on the next stage, here we displayed xylanase A, β-xylosidase, and an aldose sugar dehydrogenase on Sup35(1-61) nanofibrils to convert beechwood xylan to xylonolactone. We characterized this enzymatic cascade by measuring the time-dependent accumulation of xylose, xylooligomers, and xylonolactone. Furthermore, we studied the effects of relative enzyme concentrations, pH, temperature, and agitation on product formation. Our investigations revealed that a modular cascade with a mixture of xylanase and β-xylosidase, followed by product removal and separate oxidation of xylose with the aldose sugar dehydrogenase, is more productive than an enzyme mix containing all of these enzymes together. Moreover, we found that the nanofibril-coupled enzymes do not lose activity compared with their native state. These findings provide proof of concept of the feasibility of functionalized Sup35(1-61) fibrils as a molecular scaffold for biocatalytic cascades consisting of reusable enzymes that can be used in biotechnology. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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14. Improving the thermal stability of cellobiohydrolase Cel7A from Hypocrea jecorina by directed evolution.
- Author
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Goedegebuur, Frits, Dankmeyer, Lydia, Gualfetti, Peter, Karkehabadi, Saeid, Hansson, Henrik, Jana, Suvamay, Huynh, Vicky, Kelemen, Bradley R., Kruithof, Paulien, Larenas, Edmund A., Teunissen, Pauline J. M., Ståhlberg, Jerry, Payne, Christina M., Mitchinson, Colin, and Sandgren, Mats
- Subjects
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HYPOCREACEAE , *BIOMASS , *ENZYMES , *CELLULOSE , *XYLANASES , *THERMAL stability , *MONOOXYGENASES - Abstract
Secreted mixtures of Hypocrea jecorina cellulases are able to efficiently degrade cellulosic biomass to fermentable sugars at large, commercially relevant scales. H. jecorina Cel7A, cellobiohydrolase I, from glycoside hydrolase family 7, is the workhorse enzyme of the process. However, the thermal stability of Cel7A limits its use to processes where temperatures are no higher than 50 °C. Enhanced thermal stability is desirable to enable the use of higher processing temperatures and to improve the economic feasibility of industrial biomass conversion. Here, we enhanced the thermal stability of Cel7A through directed evolution. Sites with increased thermal stability properties were combined, and a Cel7A variant (FCA398) was obtained, which exhibited a 10.4 °C increase in Tm and a 44-fold greater half-life compared with the wild-type enzyme. This Cel7A variant contains 18 mutated sites and is active under application conditions up to at least 75 °C. The X-ray crystal structure of the catalytic domain was determined at 2.1 Å resolution and showed that the effects of the mutations are local and do not introduce major backbone conformational changes. Molecular dynamics simulations revealed that the catalytic domain of wild-type Cel7A and the FCA398 variant exhibit similar behavior at 300 K, whereas at elevated temperature (475 and 525 K), the FCA398 variant fluctuates less and maintains more native contacts over time. Combining the structural and dynamic investigations, rationales were developed for the stabilizing effect at many of the mutated sites. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
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15. Structural and Electronic Snapshots during the Transition from a Cu(II) to Cu(I) Metal Center of a Lytic Polysaccharide Monooxygenase by X-ray Photoreduction.
- Author
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Gudmundsson, Mikael, Seonah Kim, Miao Wu, Takuya Ishida, Momeni, Majid Hadadd, Vaaje-Kolstad, Gustav, Lundberg, Daniel, Royant, Antoine, Ståhlberg, Jerry, Eijsink, Vincent G. H., Beckham, Gregg T., and Sandgren, Mats
- Subjects
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MONOOXYGENASES , *OXIDATION states , *ENZYMES , *ENTEROCOCCUS faecalis , *OXIDATION - Abstract
Lytic polysaccharide monooxygenases (LPMOs) are a recently discovered class of enzymes that employ a copper-mediated, oxidative mechanism to cleave glycosidic bonds. The LPMO catalytic mechanism likely requires that molecular oxygen first binds to Cu(I), but the oxidation state in many reported LPMO structures is ambiguous, and the changes in the LPMO active site required to accommodate both oxidation states of copper have not been fully elucidated. Here, a diffraction data collection strategy minimizing the deposited x-ray dose was used to solve the crystal structure of a chitin-specific LPMO from Enterococcus faecalis (EfaCBM33A) in the Cu(II)-bound form. Subsequently, the crystalline protein was photoreduced in the x-ray beam, which revealed structural changes associated with the conversion from the initial Cu(II)-oxidized form with two coordinated water molecules, which adopts a trigonal bipyramidal geometry, to a reduced Cu(I) form in a T-shaped geometry with no coordinated water molecules. A comprehensive survey of Cu(II) and Cu(I) structures in the Cambridge Structural Database unambiguously shows that the geometries observed in the least and most reduced structures reflect binding of Cu(II) and Cu(I), respectively. Quantum mechanical calculations of the oxidized and reduced active sites reveal little change in the electronic structure of the active site measured by the active site partial charges. Together with a previous theoretical investigation of a fungal LPMO, this suggests significant functional plasticity in LPMO active sites. Overall, this study provides molecular snapshots along the reduction process to activate the LPMO catalytic machinery and provides a general method for solving LPMO structures in both copper oxidation states. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
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16. Loop Motions Important to Product Expulsion in the Thermobifida fusca Glycoside Hydrolase Family 6 Cellobiohydrolase from Structural and Computational Studies.
- Author
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Miao Wu, Lintao Bu, Thu V. Vuong, Wilson, David B., Crowley, Michael F., Sandgren, Mats, Ståhlberg, Jerry, Beckham, Gregg T., and Hansson, Henrik
- Subjects
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GLYCOSIDASES , *HYDROLASES , *CELLULOSE 1,4-beta-cellobiosidase , *CELLULASE , *ENZYMES - Abstract
Cellobiohydrolases (CBHs) are typically major components of natural enzyme cocktails for biomass degradation. Their active sites are enclosed in a tunnel, enabling processive hydrolysis of cellulose chains. Glycoside hydrolase Family 6 (GH6) CBHs act from nonreducing ends by an inverting mechanism and are present in many cellulolytic fungi and bacteria. The bacterial Thermobifida fusca Cel6B (TfuCel6B) exhibits a longer and more enclosed active site tunnel than its fungal counterparts. Here, we determine the structures of two TfuCel6B mutants co-crystallized with cellobiose, D274A (catalytic acid), and the double mutant D226A/S232A, which targets the putative catalytic base and a conserved serine that binds the nucleophilic water. The ligand binding and the structure of the active site are retained when compared with the wild type structure, supporting the hypothesis that these residues are directly involved in catalysis. One structure exhibits crystallographic waters that enable construction of a model of the α-anomer product after hydrolysis. Interestingly, the product sites of TfuCel6B are completely enclosed by an "exit loop" not present in fungal GH6 CBHs and by an extended "bottom loop". From the structures, we hypothesize that either of the loops enclosing the product subsites in the TfuCel6B active site tunnel must open substantially for product release. With simulation, we demonstrate that both loops can readily open to allow product release with equal probability in solution or when the enzyme is engaged on cellulose. Overall, this study reveals new structural details of GH6 CBHs likely important for functional differences among enzymes from this important family. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
17. Improved bio-energy yields via sequential ethanol fermentation and biogas digestion of steam exploded oat straw
- Author
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Dererie, Debebe Yilma, Trobro, Stefan, Momeni, Majid Haddad, Hansson, Henrik, Blomqvist, Johanna, Passoth, Volkmar, Schnürer, Anna, Sandgren, Mats, and Ståhlberg, Jerry
- Subjects
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BIOMASS energy , *ETHANOL , *FERMENTATION , *BIOGAS , *DIGESTER gas , *STEAM , *THERMOCHEMISTRY , *ENZYMES , *SACCHARIDES - Abstract
Abstract: Using standard laboratory equipment, thermochemically pretreated oat straw was enzymatically saccharified and fermented to ethanol, and after removal of ethanol the remaining material was subjected to biogas digestion. A detailed mass balance calculation shows that, for steam explosion pretreatment, this combined ethanol fermentation and biogas digestion converts 85–87% of the higher heating value (HHV) of holocellulose (cellulose and hemicellulose) in the oat straw into biofuel energy. The energy (HHV) yield of the produced ethanol and methane was 9.5–9.8MJ/(kg dry oat straw), which is 28–34% higher than direct biogas digestion that yielded 7.3–7.4MJ/(kg dry oat straw). The rate of biogas formation from the fermentation residues was also higher than from the corresponding pretreated but unfermented oat straw, indicating that the biogas digestion could be terminated after only 24days. This suggests that the ethanol process acts as an additional pretreatment for the biogas process. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF
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