1. CD133 is a marker for long-term repopulating murine epidermal stem cells.
- Author
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Charruyer A, Strachan LR, Yue L, Toth AS, Cecchini G, Mancianti ML, and Ghadially R
- Subjects
- AC133 Antigen, Animals, Biomarkers metabolism, Cell Differentiation physiology, Cell Proliferation, Fibroblasts cytology, Fibroblasts physiology, Flow Cytometry, Green Fluorescent Proteins genetics, Integrin alpha6 metabolism, Keratinocytes physiology, Membrane Potentials physiology, Mice, Mice, Inbred C57BL, Mice, Inbred NOD, Mice, SCID, Mice, Transgenic, Multipotent Stem Cells physiology, Regeneration physiology, Skin Transplantation, Transplantation, Homologous, Antigens, CD metabolism, Epidermal Cells, Epidermis physiology, Glycoproteins metabolism, Keratinocytes cytology, Multipotent Stem Cells cytology, Peptides metabolism
- Abstract
Maintenance, repair, and renewal of the epidermis are thought to depend on a pool of dedicated epidermal stem cells (EpiSCs). Like for many somatic tissues, isolation of a nearly pure population of stem cells is a primary goal in cutaneous biology. We used a quantitative transplantation assay, using injection of keratinocytes into subcutis combined with limiting dilution analysis, to assess the long-term repopulating ability of putative murine EpiSC populations. Putative EpiSC populations were isolated by FACS sorting. The CD133(+) population and the subpopulation of CD133(+) cells that exhibits high mitochondrial membrane potential (DΨm(hi)) were enriched for long-term repopulating EpiSCs versus unfractionated cells (3.9- and 5.2-fold, respectively). Evidence for self-renewal capacity was obtained by serial transplantation of long-term epidermal repopulating units derived from CD133(+) and CD133(+)ΔΨm(hi) keratinocytes. CD133(+) keratinocytes were multipotent and produced significantly more hair follicles than CD133(-) cells. CD133(+) cells were a subset of the previously described integrin α6(+)CD34(+) bulge cell population, and 28.9±8.6% were label-retaining cells. Thus, murine keratinocytes within the CD133(+) and CD133(+)ΔΨm(hi) populations contain EpiSCs that regenerate the epidermis for the long term, are self-renewing, multipotent, and label-retaining cells.
- Published
- 2012
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