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The aim of this study was to compare the use and effectiveness of two different stains, one fixative solution and Motile Sperm Organelle Morphology Examination (MSOME) on morphological characteristics of spermatozoa in fresh dog semen samples from epididymis. After routine castration, cauda epididymides were collected from 20 dog testes. Morphological abnormalities were evaluated by using Hancock's buffered formol saline solution, the aniline blue stain, Diff-Quik stain and MSOME analysis in epididymal semen. Conventional semen analysies and MSOME were simultaneously performed on the same sample from each dog. Percentage of abnormal spermatozoa, head abnormality and acrosomal defects were significantly higher in samples with two different staining methods and one fixative solution than MSOME technique. It was concluded that the usage of the Aniline blue stain may be an efficient method for evaluating the sperm morphology of dog semen. Although no correlation was established amoung four different methods on sperm morphology assessment. [ABSTRACT FROM AUTHOR]