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Your search keyword '"Alberto Tannús"' showing total 3 results
Start Over Author "Alberto Tannús" Topic epilepsy
3 results on '"Alberto Tannús"'

1. Manganese-enhanced magnetic resonance imaging in the acute phase of the pilocarpine-induced model of epilepsy

Author

Jackeline Moraes Malheiros, Beatriz Monteiro Longo, Alberto Tannús, and Luciene Covolan

Subjects
Epilepsy, Hippocampus, Magnetic resonance imaging, Manganese, Medicine
Abstract

Magnetic resonance images are useful in the study of experimental models of temporal lobe epilepsy. The manganese-enhanced MRI (MEMRI) technique is of interest since it combines the effects caused by manganese on the increased contrast in activated cell populations, when competing with calcium in synaptic transmission. Thus, the purpose of this study was to investigate the temporal evolution of the contrast related to manganese in the acute phase of temporal lobe epilepsy induced by systemic pilocarpine and compare it to the expression of the c-Fos protein. During this phase, the intensity of the MEMRI signal was analyzed at three different time points (5, 15 or 30 minutes) after the onset of status epilepticus (SE). The group that was maintained in status epilepticus for 30 minutes showed a decrease in intensity of the signal in CA1 and the dentate gyrus (DG). There were no differences between the control group and the other groups treated with pilocarpine. The expression of the protein, c-Fos, in the same animals showed that even in the short-duration status epilepticus (5 minutes), there was already maximal cellular activation in subregions of the hippocampus (DG, CA1 and CA3). Under the experimental conditions tested, our data suggest that the MEMRI signal was not sensitive for the identification of detectable variations of cell activation in the acute phase of the pilocarpine model. Our findings are not consistent with the idea that manganese contrast reflects primarily alterations in cellular activity during SE when other signal-modifying elements can act.

Published
2012

2. Characterization of Intracranial Pressure Behavior in Chronic Epileptic Animals: A Preliminary Study

Author

Danilo Augusto, Cardim, Gustavo Henrique, Frigieri, Brenno Caetano Troca, Cabella, Jackeline Moraes, Malheiros, Ana Carolina, Cardim, Charles Chenwei, Wang, Rodrigo, de Albuquerque Pacheco Andrade, Luciene, Covolan, Alberto, Tannús, and Sérgio, Mascarenhas

Subjects
Male, Epilepsy, Intracranial Pressure, Pilocarpine, Brain, Organ Size, Muscarinic Agonists, Hippocampus, Magnetic Resonance Imaging, Rats, Disease Models, Animal, Epilepsy, Temporal Lobe, Chronic Disease, Animals, Anticonvulsants, Rats, Wistar, Thiopental
Abstract

Intracranial pressure (ICP) is a major neurological parameter in animals and humans. ICP is a function of the relationship between the contents of the cranium (brain parenchyma, cerebrospinal fluid, and blood) and the volume of the skull. Increased ICP can cause serious physiological effects or even death in patients who do not quickly receive proper care, which includes ICP monitoring. Epilepsies are a set of central nervous system disorders resulting from abnormal and excessive neuronal discharges, usually associated with hypersynchronism and/or hyperexcitability. Temporal lobe epilepsy (TLE) is one of the most common forms of epilepsy and is also refractory to medication. ICP characteristics of subjects with epilepsy have not been elucidated because there are few studies associating these two important neurological factors. In this work, an invasive (ICPi) and the new minimally invasive (ICPmi) methods were used to evaluate ICP features in rats with chronic epilepsy, induced by the experimental model of pilocarpine, capable of generating the main features of human TLE in these animals.

Published
2016

3. Manganese-enhanced magnetic resonance imaging detects mossy fiber sprouting in the pilocarpine model of epilepsy

Author

Jackeline M, Malheiros, Roberson S, Polli, Fernando F, Paiva, Beatriz M, Longo, Luiz E, Mello, Afonso C, Silva, Alberto, Tannús, and Luciene, Covolan

Subjects
Male, Protein Synthesis Inhibitors, Manganese, Epilepsy, Pilocarpine, Muscarinic Agonists, Hippocampus, Magnetic Resonance Imaging, Statistics, Nonparametric, Article, Rats, Disease Models, Animal, Mossy Fibers, Hippocampal, Image Processing, Computer-Assisted, Animals, Anticonvulsants, Drug Interactions, Cycloheximide, Rats, Wistar, Thiopental
Abstract

Mossy fiber sprouting (MFS) is a frequent finding following status epilepticus (SE). The present study aimed to test the feasibility of using manganese-enhanced magnetic resonance imaging (MEMRI) to detect MFS in the chronic phase of the well-established pilocarpine (Pilo) rat model of temporal lobe epilepsy (TLE).To modulate MFS, cycloheximide (CHX), a protein synthesis inhibitor, was coadministered with Pilo in a subgroup of animals. In vivo MEMRI was performed 3 months after induction of SE and compared to the neo-Timm histologic labeling of zinc mossy fiber terminals in the dentate gyrus (DG).Chronically epileptic rats displaying MFS as detected by neo-Timm histology had a hyperintense MEMRI signal in the DG, whereas chronically epileptic animals that did not display MFS had minimal MEMRI signal enhancement compared to nonepileptic control animals. A strong correlation (r = 0.81, p0.001) was found between MEMRI signal enhancement and MFS.This study shows that MEMRI is an attractive noninvasive method for detection of mossy fiber sprouting in vivo and can be used as an evaluation tool in testing therapeutic approaches to manage chronic epilepsy.

Published
2012

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