1. Pitx2-Sox2-Lef1 interactions specify progenitor oral/dental epithelial cell signaling centers.
- Author
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Yu W, Sun Z, Sweat Y, Sweat M, Venugopalan SR, Eliason S, Cao H, Paine ML, and Amendt BA
- Subjects
- Adaptor Proteins, Signal Transducing genetics, Adaptor Proteins, Signal Transducing metabolism, Animals, Cell Cycle Proteins genetics, Cell Cycle Proteins metabolism, Cell Differentiation, Cell Proliferation, Dental Enamel metabolism, Embryo, Mammalian metabolism, Epithelial Cells cytology, Gene Expression Regulation, Developmental, Hedgehog Proteins metabolism, Homeodomain Proteins genetics, Lymphoid Enhancer-Binding Factor 1 genetics, Mice, Mice, Knockout, Odontogenesis, SOXB1 Transcription Factors genetics, Stem Cell Niche, Stem Cells cytology, Stem Cells metabolism, Tooth cytology, Tooth growth & development, Tooth metabolism, Transcription Factors deficiency, Transcription Factors genetics, YAP-Signaling Proteins, Homeobox Protein PITX2, Epithelial Cells metabolism, Homeodomain Proteins metabolism, Lymphoid Enhancer-Binding Factor 1 metabolism, SOXB1 Transcription Factors metabolism, Signal Transduction, Transcription Factors metabolism
- Abstract
Epithelial signaling centers control epithelial invagination and organ development, but how these centers are specified remains unclear. We report that Pitx2 (the first transcriptional marker for tooth development) controls the embryonic formation and patterning of epithelial signaling centers during incisor development. We demonstrate using Krt14
Cre / Pitx2flox/flox ( Pitx2cKO ) and Rosa26CreERT /Pitx2flox/flox mice that loss of Pitx2 delays epithelial invagination, and decreases progenitor cell proliferation and dental epithelium cell differentiation. Developmentally, Pitx2 regulates formation of the Sox2+ labial cervical loop (LaCL) stem cell niche in concert with two signaling centers: the initiation knot and enamel knot. The loss of Pitx2 disrupted the patterning of these two signaling centers, resulting in tooth arrest at E14.5. Mechanistically, Pitx2 transcriptional activity and DNA binding is inhibited by Sox2, and this interaction controls gene expression in specific Sox2 and Pitx2 co-expression progenitor cell domains. We demonstrate new transcriptional mechanisms regulating signaling centers by Pitx2 , Sox2 , Lef1 and Irx1 ., Competing Interests: Competing interestsThe authors declare no competing or financial interests., (© 2020. Published by The Company of Biologists Ltd.)- Published
- 2020
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