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Your search keyword '"Iturri, Lorea"' showing total 3 results
Start Over Author "Iturri, Lorea" Topic erythropoiesis
3 results on '"Iturri, Lorea"'

1. Yolk sac, but not hematopoietic stem cell-derived progenitors, sustain erythropoiesis throughout murine embryonic life.

Author

Soares-da-Silva F, Freyer L, Elsaid R, Burlen-Defranoux O, Iturri L, Sismeiro O, Pinto-do-Ó P, Gomez-Perdiguero E, and Cumano A

Subjects
Animals, Cell Lineage genetics, Erythropoietin metabolism, Female, Macrophages metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, Pregnancy, Proto-Oncogene Proteins c-myb deficiency, Proto-Oncogene Proteins c-myb genetics, Embryonic Development genetics, Erythrocytes metabolism, Erythropoiesis, Megakaryocyte Progenitor Cells metabolism, Yolk Sac physiology
Abstract

In the embryo, the first hematopoietic cells derive from the yolk sac and are thought to be rapidly replaced by the progeny of hematopoietic stem cells. We used three lineage-tracing mouse models to show that, contrary to what was previously assumed, hematopoietic stem cells do not contribute significantly to erythrocyte production up until birth. Lineage tracing of yolk sac erythromyeloid progenitors, which generate tissue resident macrophages, identified highly proliferative erythroid progenitors that rapidly differentiate after intra-embryonic injection, persisting as the major contributors to the embryonic erythroid compartment. We show that erythrocyte progenitors of yolk sac origin require 10-fold lower concentrations of erythropoietin than their hematopoietic stem cell-derived counterparts for efficient erythrocyte production. We propose that, in a low erythropoietin environment in the fetal liver, yolk sac-derived erythrocyte progenitors efficiently outcompete hematopoietic stem cell progeny, which fails to generate megakaryocyte and erythrocyte progenitors., Competing Interests: Disclosures: The authors declare no competing interests exist., (© 2021 Soares-da-Silva et al.)

Published
2021
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2. Megakaryocyte production is sustained by direct differentiation from erythromyeloid progenitors in the yolk sac until midgestation

Author

Iturri, Lorea, Freyer, Laina, Biton, Anne, Dardenne, Pascal, Lallemand, Yvan, Gomez Perdiguero, Elisa, Macrophages et Cellules endothéliales / Macrophages and Endothelial Cells, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Collège doctoral [Sorbonne universités], Sorbonne Université (SU), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, This work was supported by recurrent funding from the Institut Pasteur , the CNRS , and Revive (Investissement d’Avenir, ANR-10-LABX-73 ), and by an ERC investigator award ( 2016-StG-715320 ) from the European Research Council to E.G.P. E.G.P. also acknowledges financial support from the Fondation Schlumberger (FRM FSER 2017) and the Emergence(s) program from Ville de Paris (2016 DAE 190). L.I. was supported by a fellowship from the Revive Labex and L.F. by a Florence Gould-Pasteur Foundation fellowship., We gratefully acknowledge the Plateforme de Cytometrie (Sandrine Schmutz and Sophie Novault) at the Center for Innovation and Technological Research of the Institut Pasteur for support in conducting this study. We thank Yann Loe Mie and Baptiste Saudemont for their help with the MARS-Seq pipeline., ANR-10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010), European Project: 715320,ERC-2016-STG,ResidentMacroPhage(2016), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Collège Doctoral

Subjects
Male, resident macrophages, Erythrocytes, [SDV]Life Sciences [q-bio], embryo, mast cells, Article, Monocytes, Mice, Pregnancy, Animals, Cell Lineage, Myeloid Cells, [SDV.BDD]Life Sciences [q-bio]/Development Biology, Cells, Cultured, yolk sac, Macrophages, Multipotent Stem Cells, Stem Cells, Cell Differentiation, scRNAseq, Embryo, Mammalian, Hematopoietic Stem Cells, hematopoiesis, Mice, Inbred C57BL, embryonic structures, Female, fate mapping, Megakaryocytes, megakaryopoiesis, erythropoiesis, Granulocytes, fetal liver
Abstract

Summary The extra-embryonic yolk sac contains the first definitive multipotent hematopoietic cells, denominated erythromyeloid progenitors. They originate in situ prior to the emergence of hematopoietic stem cells and give rise to erythroid, monocytes, granulocytes, mast cells and macrophages, the latter in a Myb transcription factor-independent manner. We uncovered here the heterogeneity of yolk sac erythromyeloid progenitors, at the single cell level, and discriminated multipotent from committed progenitors, prior to fetal liver colonization. We identified two temporally distinct megakaryocyte differentiation pathways. The first occurs in the yolk sac, bypasses intermediate bipotent megakaryocyte-erythroid progenitors and, similar to the differentiation of macrophages, is Myb-independent. By contrast, the second originates later, from Myb-dependent bipotent progenitors expressing Csf2rb and colonize the fetal liver, where they give rise to megakaryocytes and to large numbers of erythrocytes. Understanding megakaryocyte development is crucial as they play key functions during vascular development, in particular in separating blood and lymphatic networks., Graphical abstract, Highlights • EMP produce locally committed progenitors prior to fetal liver colonization • Two sequential waves of EMP differentiation revealed at the single cell level • Yolk sac Mk develop mainly from EMPs in a Myb-independent manner • CD131 identifies Myb-dependent EMP-derived MEP progenitors in the yolk sac, The yolk sac produces the first blood cells during embryonic development. Iturri et al. now show the extent of local definitive hematopoietic differentiation occurring in this organ before colonization of the fetal liver and demonstrate the existence of a direct pathway of megakaryopoiesis produced by EMPs, independently of hematopoietic stem cells.

Published
2021

3. Yolk sac, but not hematopoietic stem cell–derived progenitors, sustain erythropoiesis throughout murine embryonic life

Author

Soares-Da-Silva, Francisca, Freyer, Laina, Elsaid, Ramy, Burlen-Defranoux, Odile, Iturri, Lorea, Sismeiro, Odile, Pinto-Do-Ó, Perpétua, Gomez-Perdiguero, Elisa, Cumano, Ana, Gestionnaire, Hal Sorbonne Université, Appel à projets générique - Deux progéniteurs hématopoïétiques différents établissent le compartiment de lymphocytes T: tester un nouveau paradigme du développement T. - - Twothyme2014 - ANR-14-CE11-0022 - Appel à projets générique - VALID, Bases moléculaires de la spécification vers le lignage lymphoïde : une approche comparative trans-espèce - - Epi-Dev2019 - ANR-19-CE14-0018 - AAPG2019 - VALID, Lymphocytes et Immunité - Lymphocytes and Immunity, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Universidade do Porto = University of Porto, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Macrophages et Cellules endothéliales / Macrophages and Endothelial Cells, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Cellule Pasteur UPMC, Institut Pasteur [Paris] (IP)-Sorbonne Université (SU), Sorbonne Université (SU), Pôle Biomics (C2RT), Centre de Ressources et de Recherche Technologique - Center for Technological Resources and Research (C2RT), Institut Pasteur [Paris] (IP)-Institut Pasteur [Paris] (IP), Agence Nationale de la Recherche (Twothyme, EPI-DEV)European Research Council (2016-StG-715320)Fundação para a Ciência e a Tecnologia (POCI-01-0145-FEDER-032656, PD/BD/114128/2015, POCI-0100145-FEDER-01638)Institut National de la Santé et de la Recherche Médicale (NO AWARD)Institut Pasteur (NO AWARD), ANR-14-CE11-0022,Twothyme,Deux progéniteurs hématopoïétiques différents établissent le compartiment de lymphocytes T: tester un nouveau paradigme du développement T.(2014), ANR-19-CE14-0018,Epi-Dev,Bases moléculaires de la spécification vers le lignage lymphoïde : une approche comparative trans-espèce(2019), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Universidade do Porto, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Sorbonne Université (SU), and Institut Pasteur [Paris]-Institut Pasteur [Paris]

Subjects
Mice, Knockout, Erythrocytes, Macrophages, [SDV]Life Sciences [q-bio], Stem Cells & Regeneration, Embryonic Development, Article, Hematopoiesis, Mice, Inbred C57BL, [SDV] Life Sciences [q-bio], Mice, Proto-Oncogene Proteins c-myb, Pregnancy, embryonic structures, Animals, Cell Lineage, Erythropoiesis, Female, Erythropoietin, Megakaryocyte Progenitor Cells, Yolk Sac
Abstract

Yolk sac erythropoiesis is not replaced by hematopoietic stem cell–derived progeny up until birth. Yolk sac–derived erythroid progenitors require 10-fold lower concentrations of erythropoietin for differentiation, which confers a competitive advantage over progenitors of hematopoietic stem cell origin., In the embryo, the first hematopoietic cells derive from the yolk sac and are thought to be rapidly replaced by the progeny of hematopoietic stem cells. We used three lineage-tracing mouse models to show that, contrary to what was previously assumed, hematopoietic stem cells do not contribute significantly to erythrocyte production up until birth. Lineage tracing of yolk sac erythromyeloid progenitors, which generate tissue resident macrophages, identified highly proliferative erythroid progenitors that rapidly differentiate after intra-embryonic injection, persisting as the major contributors to the embryonic erythroid compartment. We show that erythrocyte progenitors of yolk sac origin require 10-fold lower concentrations of erythropoietin than their hematopoietic stem cell–derived counterparts for efficient erythrocyte production. We propose that, in a low erythropoietin environment in the fetal liver, yolk sac–derived erythrocyte progenitors efficiently outcompete hematopoietic stem cell progeny, which fails to generate megakaryocyte and erythrocyte progenitors.

Published
2021

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