1. Effects of erythropoietin on STAT1 and STAT3 levels following cerebral ischemia-reperfusion in rats.
- Author
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Jiang C, Xu Q, Xu K, Dai H, Zhang Z, Wu W, and Ni J
- Subjects
- Animals, Apoptosis drug effects, Blotting, Western, Epoetin Alfa, Immunohistochemistry, In Situ Nick-End Labeling, Infarction, Middle Cerebral Artery pathology, Male, Neurologic Examination, Rats, Rats, Sprague-Dawley, Recombinant Proteins pharmacology, Erythropoietin pharmacology, Hematinics pharmacology, Reperfusion Injury metabolism, STAT1 Transcription Factor biosynthesis, STAT3 Transcription Factor biosynthesis
- Abstract
Background and Purpose: Cerebral ischemia-reperfusion injury can activate signal transducers and activators of transcription (STAT). STAT1 initiates neuronal apoptosis following cerebral ischemia-reperfusion, while STAT3 is neuroprotective. Erythropoietin (EPO) promotes regeneration through STAT3 and facilitates neuronal survival following ischemia. However, there are few reports on the effects of EPO on phosphorylated STAT1 (P-STAT1) level following cerebral ischemia-reperfusion in rats, and there is no evidence on the simultaneous observation of the four kinds of protein:STAT1, P-STAT1, STAT3, and P-STAT3., Methods: We established a rat focal cerebral ischemia-reperfusion injury model, and used Western blot and immunohistochemical staining to assess the levels of STAT1 and STAT3 expression, and TdT-mediated dUTP-biotin nick end-labeling (TUNEL) was carried out to observe the number of apoptotic cells with or without EPO treatment., Results: Our findings show that EPO treatment had no significant effect on STAT1 and STAT3 expression, but P-STAT1 and P-STAT3 were slightly decreased and significantly increased, respectively, after EPO treatment. Neurologic deficits, the infarct volume, and the number of apoptotic cells were significantly decreased after EPO treatment., Conclusions: The results suggest that EPO exerts a neuroprotective effect by influencing STAT3 and STAT1 expression in the area injured by cerebral ischemia-reperfusion.
- Published
- 2013
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