1. A systematic approach to isolate mono-disperse membrane proteins - purification of zinc transporter ZntB.
- Author
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Van Pham ST, Engman H, Dahlgren LG, Cornvik T, and Eshaghi S
- Subjects
- Amino Acid Sequence, Bacterial Proteins chemistry, Carrier Proteins chemistry, Detergents, Membrane Proteins chemistry, Membrane Proteins genetics, Membrane Proteins isolation & purification, Molecular Sequence Data, Salmonella typhimurium chemistry, Sequence Alignment, Solubility, Carrier Proteins genetics, Carrier Proteins isolation & purification, Cloning, Molecular methods, Escherichia coli genetics
- Abstract
Obtaining mono-disperse and stable protein is a requirement for successful structural and biochemical investigation of proteins. For membrane proteins, such preparation is one of the major hurdles, which consequently has contributed to the slow progress in studying them. During the past few years, many screening methods have been developed to make studies of membrane proteins more efficient. Despite these advances, many membrane proteins remain challenging to even isolate in a stable and homogeneous form. The bacterial zinc transporter ZntB is such a protein, for which no isolation procedure has been reported. Here, we present a systematic approach to obtain homogeneous and mono-disperse zinc transporter ZntB in quantities sufficient for structural and biochemical studies. Important aspects of this study that can be applied to other membrane proteins are also discussed., (Copyright 2010 Elsevier Inc. All rights reserved.)
- Published
- 2010
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