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Start Over Author "Happe, Birgitta" Topic escherichia coli
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1. Genetic and biochemical characterization of the broad spectrum chlorobenzene dioxygenase from Burkholderia sp. strain PS12: Dechlorination of 1,2,4,5-tetrachlorobenzene.

Author

Beil, Stefan, Happe, Birgitta, Timmis, Kenneth Nigel, and Pieper, Dietmar Helmut

Subjects
*CHLOROBENZENE, *DNA, *ESCHERICHIA coli, *PROTEINS, *BACTERIA, *GENE expression
Abstract

Reports on the bacterium, Burkholderia sp. strain PS12 which uses 1,2,4,5-tetrachlorobenzene (Cl[sub 4]-benzene) as a growth substrate. Cloning of a 5503-bp DNA fragment from PS12 and expression in Escherichia coli; Inclusion of the tec genes coding for the alpha-subunit and beta-subunit of a terminal dioxygenase, a ferrodoxin and a reductase; Proof that dioxygenase caused dechlorination of Cl[sub 4]-benzene.

Published
1997
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